Fermentation optimization of cellulase production from sugarcane bagasse by Bacillus pseudomycoides and molecular modeling study of cellulase

Pramanik, Sajib Kumar; Mahmud, Shafi; Paul, Gobindo Kumar; Jabin, Tabassum; Naher, Kamrun; Uddin, Md. Salah; Zaman, Shahriar; Saleh, Md. Abu

doi:10.1016/j.crmicr.2020.100013

Cited by 45 publications

(35 citation statements)

References 31 publications

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“…The simulation trajectories were saved after every 100 ps and the final simulation run was conducted for 100 ns ( Krieger and Vriend, 2015 ). The simulation trajectories were utilized to analyze the root-mean-square deviation, the solvent-accessible surface area, the radius of gyration, and hydrogen bonding ( Chowdhury et al, 2020 , Munia et al, 2021 , Pramanik et al, 2021 , Rakib et al, 2021 , Uddin et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria

Paul

Mahmud

Hasan

et al. 2021

Saudi Journal of Biological Sciences

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Aphanamixis polystachya may be a natural, renewable resource against antibiotic-resistant bacterial infections. The antibacterial activity of A. polystachya leaf and bark extracts was investigated against three antibiotic-resistant bacterial species and one fungus. Methanolic leaf extract showed only limited antibacterial activity but both methanolic and aqueous bark extract showed high antimicrobial activity. In an antioxidant activity test, leaf and bark extracts exhibited 50% free radical scavenging at a concentration of 107.14 ± 3.14 μg/mL and 97.13 ± 3.05 μg/mL, respectively, indicating that bark extracts offer more antioxidative activity than leaf extracts. Bark extracts also showed lower toxicity than leaf extracts. This suggests that bark extracts may offer greater development potential than leaf extracts. The molecular dynamics were also investigated through the simulated exploration of multiple potential interactions to understand the interaction dynamics (root-mean-square deviation, solvent-accessible surface area, radius of gyration, and the hydrogen bonding of chosen compounds to protein targets) and possible mechanisms of inhibition. This molecular modeling of compounds derived from A. polystachya revealed that inhibition may occur by binding to the active sites of the target proteins of the tested bacterial strains. A. polystachya bark extract may be used as a natural source of drugs to control antibiotic-resistant bacteria.

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Section: Methodsmentioning

confidence: 99%

Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria

Paul

Mahmud

Hasan

et al. 2021

Saudi Journal of Biological Sciences

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show abstract

“…The final simulation was ran for 100 ns ( Krieger and Vriend, 2015 ) while adhering to constant pressure and Berendsen thermostat. The root mean square deviation, solvent accessible surface area, radius of gyration, and hydrogen bond were studied using simulation trajectories ( Afrose et al, 2021 , Chowdhury et al, 2021 , Mahmud et al, 2021d , 2021e, 2021a; “Molecular docking and dynamics study to explore phytochemical ligand molecules against the main protease of SARS-CoV-2 from extensive phytochemical datasets,” n.d., “Screening of Potent Phytochemical Inhibitors Against SARS-CoV-2 Main Protease: An Integrative Computational Approach,” n.d.; Pramanik et al, 2021 , Rakib et al, 2021 , Uddin et al, 2021 )…”

Section: Methodsmentioning

confidence: 99%

Computational screening and biochemical analysis of Pistacia integerrima and Pandanus odorifer plants to find effective inhibitors against Receptor-Binding domain (RBD) of the spike protein of SARS-Cov-2

Paul

Mahmud

Aldahish

et al. 2022

Arabian Journal of Chemistry

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“…Cellulase activity of the bacterial strains was measured by the following method [ 40 ]. The reaction mixtures containing 1.8 mL of 0.5% carboxymethyl cellulose (CMC) were added to 50 mM sodium phosphate buffer (pH 7) and 0.2 mL of crude enzyme in screw capped tubes, and were incubated at 20 °C in a water bath for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Psychrotolerant Mesorhizobium sp. Isolated from Temperate and Cold Desert Regions Solubilizes Potassium and Produces Multiple Plant Growth Promoting Metabolites

et al. 2021

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Soil potassium (K) supplement depends intensively on the application of chemical fertilizers, which have substantial harmful environmental effects. However, some bacteria can act as inoculants by converting unavailable and insoluble K forms into plant-accessible forms. Such bacteria are an eco-friendly approach for enhancing plant K absorption and consequently reducing utilization of chemical fertilization. Therefore, the present research was undertaken to isolate, screen, and characterize the K solubilizing bacteria (KSB) from the rhizosphere soils of northern India. Overall, 110 strains were isolated, but only 13 isolates showed significant K solubilizing ability by forming a halo zone on solid media. They were further screened for K solubilizing activity at 0 °C, 1 °C, 3 °C, 5 °C, 7 °C, 15 °C, and 20 °C for 5, 10, and 20 days. All the bacterial isolates showed mineral K solubilization activity at these different temperatures. However, the content of K solubilization increased with the upsurge in temperature and period of incubation. The isolate KSB (Grz) showed the highest K solubilization index of 462.28% after 48 h of incubation at 20 °C. The maximum of 23.38 µg K/mL broth was solubilized by the isolate KSB (Grz) at 20 °C after 20 days of incubation. Based on morphological, biochemical, and molecular characterization (through the 16S rDNA approach), the isolate KSB (Grz) was identified as Mesorhizobium sp. The majority of the strains produced HCN and ammonia. The maximum indole acetic acid (IAA) (31.54 µM/mL) and cellulase (390 µM/mL) were produced by the isolate KSB (Grz). In contrast, the highest protease (525.12 µM/mL) and chitinase (5.20 µM/mL) activities were shown by standard strain Bacillus mucilaginosus and KSB (Gmr) isolate, respectively.

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Fermentation optimization of cellulase production from sugarcane bagasse by Bacillus pseudomycoides and molecular modeling study of cellulase

Cited by 45 publications

References 31 publications

Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria

Biochemical and in silico study of leaf and bark extracts from Aphanamixis polystachya against common pathogenic bacteria

Computational screening and biochemical analysis of Pistacia integerrima and Pandanus odorifer plants to find effective inhibitors against Receptor-Binding domain (RBD) of the spike protein of SARS-Cov-2

Psychrotolerant Mesorhizobium sp. Isolated from Temperate and Cold Desert Regions Solubilizes Potassium and Produces Multiple Plant Growth Promoting Metabolites

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