Fermentation product with new equol‐producing Lactobacillus paracasei as a probiotic‐like product candidate for prevention of skin and intestinal disorder

Abstract

BACKGROUND: Equol is a major isoflavone metabolite, and equol-producing bacteria have been isolated and characterized; however, fermentation has been performed with soybean-based products as substrates. Pueraria lobata has been reported as a plant with higher content of isoflavones. RESULTS:The genome of new equol-producing bacteria, Lactobacillus paracasei JS1, was analyzed. Also, the effect of P. lobata extract fermented with L. paracasei JS1 (FPE) on the skin and intestinal immune response was examined. With gene expression analysis, it was proven that seven skin-related proteins, hyaluronan synthase-1, -2, -3, collagen, elastin, epidermal growth factor, and epidermal growth factor receptor were differentially expressed upon FPE treatment. The messenger RNA expression increased with treatment with the FPE, and a skin moisturizing effect was confirmed by a hematoxylin-eosin staining experiment. In addition, such an experiment showed that proinflammatory cytokines, tumor necrosis factor-, cyclooxygenase-2, inducible nitric oxide synthase, interleukin-1 , -4, and -6, were reduced in large intestine when treated with FPE. CONCLUSION: L. paracasei JS1 has the ability to produce equol having beneficial effects on the skin. Moreover, FPE also has an inhibitory effect on inflammation cytokines in the large intestine. Thus, the novel and edible equol-producing L. paracasei JS1 and FPE have thepotential to be developed as nutricosmetic resources. Experimental animalsSix-week-old male C57BL/6J mice (RaonBio, Yongin, Korea) were housed in an air-conditioned animal room over a 12-h light/dark cycle at a temperature of 22 ± 1 ∘ C and humidity of 50 ± 10%. The J Sci Food Agric 2019; 99: 4200-4210

Characterization, health benefits and applications of fruits and vegetable probiotics

“…Another skin protection enzyme, sphingomyelinase (SMase) produced by Streptococcus thermophilus delivered in exogenous probiotics formulations was reported to improve skin health and effective in treatment of dermatitis and other skin ailments (Lew & Liong, 2013). Moreover, polyphenols and antioxidants from FV as prebiotics composition play roles in skin protection and antiaging mechanisms providing synergetic effect on probiotics (Kwon et al, 2019;Ouwehand et al, 2017).…”

Microbiota Targeted Interventions of Probiotic Lactobacillus as an Anti-Ageing Approach: A Review

“…After analysis of gene expression, it was observed that the messenger RNA of seven skin-related proteins was differentially expressed. The skin moisturizing effects of the strain were also confirmed during the study [ 133 ].…”

Pueraria montana var. lobata root extract inhibits photoaging on skin through Nrf2 pathway.

“…lobata as a potential antioxidant reagent. Previously, a number of studies have been carried out, including skin whitening, use of fermented product, and anti-inflammatory effects of Pueraria lobata on skin [27][28][29]. In the present study, it was the first in which Pueraria lobata was shown to have the effect of inhibiting photoaging by activating Nrf2, a defense mechanism in the human body.…”