Fermentation von Biopolyester (PHA) im Hochdruck‐Bioreaktor

Noger, Dominik; Hartmann, René; Zinn, Manfred

doi:10.1002/cite.200650248

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…If important physiological damages can thus be excluded between 1 and 10 bar, the cells might still sense such pressures and respond to them by rearranging their metabolism, which may have negative consequences on the bioprocess productivity as well. Pressure has been tested for increasing OTR and/or product yield during fermentations with yeasts and bacteria (Belo et al, 2003; Knoll et al, 2005, 2007; Lopes et al, 2009; Noger et al, 2006). In particular, it was shown that pressure could be applied up to 11 bar without negative effects on the cell growth of Arxula adeninivorans , Saccharomyces cerevisiae , Corynebacterium glutamicum , and Escherichia coli (Knoll et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Putting cells under pressure: A simple and efficient way to enhance the productivity of medium‐chain‐length polyhydroxyalkanoate in processes with Pseudomonas putida KT2440

Follonier

Henes

Panke

et al. 2011

Biotech & Bioengineering

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The success of bioprocess implementation relies on the ability to achieve high volumetric productivities and requires working with high-cell-density cultivations. Elevated atmospheric pressure might constitute a promising tool for enhancing the oxygen transfer rate (OTR), the major growth-limiting factor for such cultivations. However, elevated pressure and its effects on the cellular environment also represent a potential source of stress for bacteria and may have negative effects on product formation. In order to determine whether elevated pressure can be applied for enhancing productivity in the case of medium-chain-length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas putida KT2440, the impact of a pressure of 7 bar on the cell physiology was assessed. It was established that cell growth was not inhibited by this pressure if dissolved oxygen tension (DOT) and dissolved carbon dioxide tension (DCT) were kept below ∼30 and ∼90 mg L(-1), respectively. Remarkably, a little increase of mcl-PHA volumetric productivity was observed under elevated pressure. Furthermore, the effect of DCT, which can reach substantial levels during high-cell-density processes run under elevated pressure, was investigated on cell physiology. A negative effect on product formation could be dismissed since no significant reduction of mcl-PHA content occurred up to a DCT of ∼540 mg L(-1). However, specific growth rate exhibited a significant decrease, indicating that successful high-cell-density processes under elevated pressure would be restricted to chemostats with low dilution rates and fed-batches with a small growth rate imposed during the final part. This study revealed that elevated pressure is an adequate and efficient way to enhance OTR and mcl-PHA productivity. We estimate that the oxygen provided to the culture broth under elevated pressure would be sufficient to triple mcl-PHA productivity in our chemostat system from 3.4 (at 1 bar) to 11 g L(-1)h(-1) (at 3.2 bar).

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Section: Introductionmentioning

confidence: 99%

Putting cells under pressure: A simple and efficient way to enhance the productivity of medium‐chain‐length polyhydroxyalkanoate in processes with Pseudomonas putida KT2440

Follonier

Henes

Panke

et al. 2011

Biotech & Bioengineering

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“…Bioprocesses under elevated pressure have been investigated with various microorganisms, including yeasts and bacteria (Absidia coerula, Arxula adeninivorans, Aureobasidium pullulans, Corynebacterium glutamicum, E. coli, Kluyveromyces marxianus, P. putida, Saccharomyces cerevisiae,a n dYarrowia lipolytica) (Aguedo et al 2005;Beloetal.2003;Dufresne et al 1990;Follonier et al 2012;Jia and Cui 2009;Knabben et al 2010a;Knabbenet al 2010b;Knoll et al 2007;Knoll et al 2005;Lopes et al 2008;Lopes et al 2009;M ae ta l .2010;M a t s u ie ta l . Noger et al 2006;Pinheiroetal.2000;YangandWang1992). In general, applying elevated pressure resulted in larger cell and product productivities (Table 1), except in some cases where growth inhibition occurred due to large DCT (Knoll et al 2005;M a t s u ie ta l .2006).…”

Section: Biotechnological Applications Based On Elevated Pressurementioning

confidence: 99%

Pressure to kill or pressure to boost: a review on the various effects and applications of hydrostatic pressure in bacterial biotechnology

Follonier

Panke

Zinn

2012

Appl Microbiol Biotechnol

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Much knowledge has been gained for the last 30 years about the effects of pressure on bacteria, and various pressure-based technologies have been designed. The development of modern molecular biology techniques (e.g., DNA microarrays) as well as the technological advances realized in the manufacturing of robust sampling and high-pressure devices has allowed these advances. Not only the direct effects on cell components (membranes, proteins, and nucleic acids) have been unraveled, but also the cellular response to pressure has been investigated by means of transcriptome and proteome analyses. Initially, research was performed by marine biologists who studied the microorganisms living in the deep sea at pressures of 1,000 bar. In parallel, food technologists developed pressure-based methods for inactivating microorganisms without altering the food properties as much as with temperature treatment. The preservation of specific product properties is also the rationale for pressure-based methods for the disinfection of biomaterials and for vaccine production. Therefore, attention was first focused on the “killing” potential of high pressure. On the other hand, there has been a growing interest in using elevated pressures (up to ~10 bar) for enhancing the productivity of bioprocesses. In this case, no killing effect was sought, but pressure was applied to “boost” the process by enhancing the oxygen transfer to the cell culture. This paper gives an overview on the effects of pressures in the range of 1 bar to 10 kbar on bacteria and presents the major and most recent achievements realized in the development of pressure-based biotechnological applications.

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Fermentation von Biopolyester (PHA) im Hochdruck‐Bioreaktor

Cited by 2 publications

References 2 publications

Putting cells under pressure: A simple and efficient way to enhance the productivity of medium‐chain‐length polyhydroxyalkanoate in processes with Pseudomonas putida KT2440

Putting cells under pressure: A simple and efficient way to enhance the productivity of medium‐chain‐length polyhydroxyalkanoate in processes with Pseudomonas putida KT2440

Pressure to kill or pressure to boost: a review on the various effects and applications of hydrostatic pressure in bacterial biotechnology

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