Ferritin from the Pacific abalone Haliotis discus hannai: Analysis of cDNA sequence, expression, and activity

Qiu, Reng; Kan, Yunchao; Li, Dandan

doi:10.1016/j.fsi.2015.12.048

Cited by 6 publications

(3 citation statements)

References 48 publications

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“…Ferritin is a soluble histone that stores iron in the body. The involvement of ferritin in the immune defense against pathogens was reported in some aquatic species, containing sea cucumber (Ren et al, 2014), Japanese scallop (Zhang et al, 2013), tiger prawn (Maiti et al, 2010), soft-shell clam (Araya et al, 2010), and abalone (He et al, 2016;Qiu et al, 2016). Moreover, this is consistent with our result that the expression of ferritin was also upregulated after challenge.…”

Section: Abalone Promote Apoptosis and Clearance Of Apoptotic Cells T...supporting

confidence: 92%

Transcriptome study on immune response against Vibrio parahaemolyticus challenge in gill of abalone Haliotis discus hannai Ino

et al. 2022

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Abalone (Haliotis discus hannai Ino) is one of the most vital economic shellfish cultured in China. However, bacterial and viral infection often leads to its high mortality and serious economic losses. In this study, transcriptome sequencing was performed on gill of abalone challenged by Vibrio parahaemolyticus for 0 h (control group, A0h), 8 h (A8h), and 48 h (A48h), respectively. Each library has an average of 53,361,675 clean reads. Approximately 85.71% reads were mapped to the reference genome of Haliotis discus hannai Ino. In comparison with A0h group, 7,656 and 2,789 genes were differentially expressed in the A8h and A48h groups, respectively. The differentially expressed genes (DEGs) were mostly enriched in oxidation–reduction process and oxidoreductase activity from GO analysis as well as immunity and metabolism-related pathways from Kyoto Encyclopedia of Genes and Genomes analysis. Cluster analysis demonstrated that all DEGs were categorized into eight subclusters by adopting the gene expression model. Specifically, a total of 1,070 significantly downregulated genes from two clusters were related to some metabolic pathways, ABC transporters, and cell adhesion. Moreover, 212 upregulated genes in one subcluster were enriched in several immune-related pathways, including nucleotide-binding oligomerization domain (NOD)-like receptor signaling pathway and apoptosis. Some members of the pattern recognition receptors family, which can initiate inflammatory signaling and induce apoptosis, were stimulated to upregulate in gill after challenge including C1q, perlucin, and low-density lipoprotein receptor–related protein. In addition, caspase-dependent apoptosis pathway was inactive, whereas caspase-independent apoptosis pathway was stimulating in response to Vibrio challenge. To eliminate invading pathogen, the immune effectors such as interleukin-17, matrix metalloproteinase, lysozyme, superoxide, and ferritin were also stimulated in gill. Moreover, this study also provides more information for comprehending the immunity and defense mechanisms against V. parahaemolyticus infection in abalone.

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Section: Abalone Promote Apoptosis and Clearance Of Apoptotic Cells T...supporting

confidence: 92%

Transcriptome study on immune response against Vibrio parahaemolyticus challenge in gill of abalone Haliotis discus hannai Ino

et al. 2022

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“…Although alanines are also frequently found at the corresponding sites of eukaryotic sequences, they are not conserved for prokaryotes . Even in eukaryotes, turbot ferritin H-chain and pacific abalone ferritin 1 have leucine and valine as a corresponding residue, respectively . Together with these, our result in which the A96T mutation can also permit successful iron incorporation indicates that alanine is not essential for this position.…”

Section: Discussionmentioning

confidence: 99%

“…25 Even in eukaryotes, turbot ferritin H-chain and pacific abalone ferritin 1 have leucine and valine as a corresponding residue, respectively. 26 Together with these, our result in which the A96T mutation can also permit successful iron incorporation indicates that alanine is not essential for this position. The three-dimensional structure of A96T was modeled using UCSF Chimera.…”

Section: ■ Discussionmentioning

confidence: 99%

Structure, Function, Folding, and Aggregation of a Neuroferritinopathy-Related Ferritin Variant

et al. 2019

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Neuroferritinopathy is a rare, adult-onset, dominantly inherited movement disorder caused by mutations in the ferritin gene. A ferritin light-chain variant related to neuroferritinopathy, in which alanine 96 is replaced with threonine (A96T), was expressed in Escherichia coli, purified, and characterized. The circular dichroism, analytical ultracentrifugation, and small-angle X-ray scattering studies have shown that both the subunit structure and the assembly of A96T are the same as those of wild-type human ferritin light chain (HuFTL). The iron-incorporation ability was also comparable to that of HuFTL. Although the structural stability against heat, acid, and denaturant was reduced, the structure was sufficiently stable under physiological conditions. The most remarkable defects observed for A96T were a lower refolding efficiency and a stronger propensity to aggregate. The possible relationship between folding deficiency and disease is discussed.

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Complete nuclear ribosomal DNA sequence analysis of Pacific abalone Haliotis discus hannai

et al. 2017

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Ferritin from the Pacific abalone Haliotis discus hannai: Analysis of cDNA sequence, expression, and activity

Cited by 6 publications

References 48 publications

Transcriptome study on immune response against Vibrio parahaemolyticus challenge in gill of abalone Haliotis discus hannai Ino

Transcriptome study on immune response against Vibrio parahaemolyticus challenge in gill of abalone Haliotis discus hannai Ino

Structure, Function, Folding, and Aggregation of a Neuroferritinopathy-Related Ferritin Variant

Complete nuclear ribosomal DNA sequence analysis of Pacific abalone Haliotis discus hannai

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