Ferrocene-mediated enzyme electrode for amperometric determination of glucose

Ae, Cass; Davis, Graham; Gd, Francis; Ha, Hill; Aston, WJ; Ij, Higgins; Ev, Plotkin; Ld, Scott; Turner, Anthony P. F.

doi:10.1021/ac00268a018

Cited by 1,596 publications

(714 citation statements)

References 14 publications

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“…50% and occurs over several hours exposure to brain tissue (Garguilo and Michael, 1994;Hu et al, 1994). Although previous in vitro studies have shown that Pt/PPD/GOx sensors are virtually free from both protein and lipid fouling (Lowry and O'Neill, 1994) we carried out an additional in vitro conditioning of the sensors prior to implantation (see Section 2.4), similar to the preconditioning procedure used by Hill and coworkers to improve the stability of ferrocene-mediated glucose biosensors (Cass et al, 1984). The mean baseline current recorded with preconditioned Pt/PPD/ GOx sensors implanted in the striatum of freely-moving animals showed no significant variation (p\ 0.88, twoway ANOVA; n= 6) over a successive 5 day period from day 0 (ca.…”

Section: Stability Of Biosensor In 6i6omentioning

confidence: 99%

An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

Lowry

Miele

O’Neill

et al. 1998

Journal of Neuroscience Methods

100

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Amperometric glucose biosensors based on the immobilization of glucose oxidase (GOx) on Pt electrodes with electropolymerized o-phenylenediamine (PPD) were implanted in the right striatum of freely-moving rats. Carbon paste electrodes for the simultaneous monitoring of ascorbic acid (AA) and/or tissue O 2 were implanted in the left striatum. A detailed in vivo characterization of the Pt/PPD/GOx signal was carried out using various pharmacological manipulations. Confirmation that the biosensor responded to changing glucose levels in brain extracellular fluid (ECF) was obtained by intraperitoneal (i.p.) injection of insulin that caused a decrease in the Pt/PPD/GOx current, and local administion of glucose (1 mM) via an adjacent microdialysis probe that resulted in an increase in the biosensor current. An insulin induced increase in tissue O 2 in the brain was also observed. Interference studies involved administering AA and subanaesthetic doses of ketamine i.p. Both resulted in increased extracellular AA levels with ketamine also causing an increase in O 2 . No significant change in the Pt/PPD/GOx current was observed in either case indicating that changes in O 2 and AA, the principal endogenous interferents, have minimal effect on the response of these first generation biosensors. Stability tests over a successive 5-day period revealed no significant change in sensitivity. These in vivo results suggest reliable glucose monitoring in brain ECF.

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Section: Stability Of Biosensor In 6i6omentioning

confidence: 99%

An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

Lowry

Miele

O’Neill

et al. 1998

Journal of Neuroscience Methods

100

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“…66 Ferrocene-modified polypyrrole was subsequently used to immobilize glucose oxidase. 6 7 -68 Highly flexible ferrocene-modified siloxanes and polyethylene oxides were also used as mediators, carrying electrons between glucose oxidase and other enzymes and high surface area carbon pastes that adsorb the enzyme and the redox polymer. 6 9 -7 4 In most of these redox-polymer systems, the polymers, often of low molecular weight (20, …”

Section: Electrical Wiring Of Enzymesmentioning

confidence: 99%

Electrical connection of enzyme redox centers to electrodes

1992

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“…Many efforts have been made to improve the selectivity of carbon based electrochemical sensors, mainly through their modification with redox mediators (Cass et al, 1984;Frew and Hill, 1987).…”

Section: Introductionmentioning

confidence: 99%

Prussian Blue based screen printed biosensors with improved characteristics of long-term lifetime and pH stability

Ricci

Amine²,

Palleschi

et al. 2003

Biosensors and Bioelectronics

329

152

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The promising advantages of Prussian Blue (PB) as catalyst and of the thick film screen printing technology have been combined to assemble sensors with improved characteristics for the amperometric determination of H 2 O 2 . PB-modified screen printed electrodes were applied to detect H 2 O 2 at an applied potential of (/0.05 V versus the internal screen printed Ag pseudoreference electrode, showing a detection limit of 10 (7 mol l (1 , a linearity range from 10 (7 to 5 )/10 (5 mol l (1 , a sensitivity of 234 mA mmol l (1 cm (2 , and a high selectivity. Improved stability at alkaline pH values was also observed, which made possible their use with enzymes having an optimum basic pH. Then, the immobilisation of a single enzyme (glucose oxidase (GOD) or choline oxidase (ChOX)) or of two enzymes, acetylcholinesterase (AchE) coimmobilised with ChOX, has been performed on the surface of PB modified screen-printed electrodes (SPEs) using glutaraldehyde and Nafion † . ChOX has been selected as an example of enzyme working at alkaline pH. The choline biosensors showed a detection limit of 5 )/10 (7 mol l (1 , a wide linearity range (5 )/10 (7 Á/ 10 (4 mol l (1 ), a high selectivity and a remarkable long term stability of 9 months at 4 8C, and at least 4 weeks at room temperature. Similar analytical characteristics and stability were observed with the acetylcholine biosensors. #

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Ferrocene-mediated enzyme electrode for amperometric determination of glucose

Cited by 1,596 publications

References 14 publications

An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

Electrical connection of enzyme redox centers to electrodes

Prussian Blue based screen printed biosensors with improved characteristics of long-term lifetime and pH stability

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