Ferroptosis Contributed Genes in the Soleus Muscle After Endurance Training in Older Rats

Abstract

The main aim of the research is to investigate the effect of endurance physical activity on ChaC Glutathione Specific Gamma-Glutamyl cyclotransferase 1 (CHAC1), Glutamate-Cysteine Ligase Catalytic subunit (GCLC), Arachidonate 12-Lipoxygenase (ALOX12), as some ferroptosis related-genes expressed in soleus muscle tissue, in order to clarify apart of muscle atrophy physiology in old rats. Twenty old Wistar male rats were randomly assigned into two groups, endurance training and control groups. The training group ran incrementally on a treadmill, five days a week (velocity: 10–28 m/min). The expression genes were investigated by Real-time PCR and Malondialdehyde (MDA) and Superoxide dismutase (SOD) level as oxidative stress predicators were measured by assay kit. The mRNA expression of CHAC1 in the training group (0.36 ± 0.06)-fold decreased as opposed to the control group (P < 0.01). Increment in mRNA levels of GCLC and ALOX12 were seen in exercise group comparing with control, respectively (1.29 ± 0.21)-fold, (P > 0.01) and (1.84 ± 0.25)-fold, (P < 0.01). The level of MDA in the training group decreased significantly (1.18 ± 0.24) compared with the control group (1.58 ± 0.32), (P < 0.01). SOD level were higher in training (17.03 ± 2.10) compared with control (1.593 ± 14.45), (P < 0.01). It seems that regular aerobic running exercise could possibly Influence rodents muscle regulation through ferroptosis process and lipid peroxidation accumulation.