Fertile Plants Regenerated from Protoplasts of Cold Tolerant Rice Line RCPL 1-2C

Bhattacharjee, Bijoya; Gupta, H. S.

doi:10.1007/bf03262954

Cited by 3 publications

(2 citation statements)

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“…The high concentration of 2,4-D (7 mg l -1 ) used for subculture could shorten the time required for embryogenic callus selection, an observation made by Wang et al (1990) for wheat. The suitability of the amino acid-based culture media AA for the rapid establishment of a rice suspension culture that readily yields protoplasts has been noted in previous studies Jenes and Pauk 1989;Bhattacharjee and Gupta 1995;Jain et al 1995;Xue and Earle 1995). In the present investigation, suspensions were successfully established in both AA medium and MS-based mMS medium.…”

Section: Discussionsupporting

confidence: 62%

“…In our experiment, the optimal plating density with the highest division frequency and plating efficiency was 110 6 protoplasts per milliliter medium, and a lower or higher density resulted in decreased division frequency and plating efficiency (Table 2). A similar density was used in barley (Jähne et al 1991;Funatsuki et al 1992) and rice (Bhattacharjee and Gupta 1995;Xue and Earle 1995;Fujimura et al 1985). KM8p medium was used for protoplast culture, and of the four hormone and sugar solutions tested in the medium, solution 1 proved to be the most effective for cell division frequency and plating efficiency (Table 3).…”

Section: Discussionmentioning

confidence: 99%

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Somatic embryogenesis and fertile green plant regeneration from suspension cell-derived protoplasts of rye ( Secale cereale L.)

Guo

Pulli

2003

Plant Cell Reports

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A method for somatic embryogenesis and fertile green plant regeneration from suspension cell-derived protoplasts of rye (Secale cereale L. cv. Auvinen) was developed. Fast-growing and friable embryogenic calli with a high regeneration capacity were induced from immature rye inflorescences using modified MS medium. These friable embryogenic calli were used for suspension culture initiation in liquid AA medium. A high yield of protoplasts was obtained from suspension cell clumps after 3-5 days of subculture. Isolated protoplasts were cultured in KM8p medium. The frequency of protoplast cell divisions and colony formations in liquid culture medium were similar to those on agarose-solidified medium. Compact embryogenic calli were developed from protoplast-derived microcalli in growth medium mMS. Approximately 7% of the transferred embryogenic calli produced green shoots on N6 regeneration medium. Of 33 green plants, 28 were fertile with normal flowering and seed set. The ratio of green and albino plantlets was 1:4. Rye protoplast-derived green plants showed normal diploid characters as determined by flow cytometer analysis and chromosome counting.

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Section: Discussionsupporting

confidence: 62%