Fertility control in laboratory rats and mice after feeding with the antigestagen RU486

Gao, Yinjie; Short, R. V.

doi:10.1530/jrf.0.1010477

Cited by 20 publications

(9 citation statements)

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“…Following 2-4 days of injection of mifepristone, the normal estrous cycles were disturbed to be 'persistent vaginal cornification' or consecutive estrous stage until the time of tissue fixation (Ruiz et al 1996). The persistent vaginal smear was consistent with the findings of previous report using the oral administration of mifepristone (Gao & Short 1994). After the ovarian tissue specimens were prepared with perfusion fixation and alcohol dehydration (PF-DH) and also IVCT, the paraffin sections from control or mifepristone-induced PCO model mice were first examined following hematoxylin-eosin (HE) staining (Fig.…”

Section: Histological Examination Of Mouse Ovaries After Injection Ofsupporting

confidence: 87%

“…Some previous studies have reported the estrous cycle and ovulation to be stopped by the administration of RU-486 in mice as observed in the present study (Loutradis et al 1991, Gao & Short 1994, and the anovulation and PCO have been included in the diagnostic criteria of PCO syndrome (The Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group 2004). During Figure 3 Light micrographs of antral follicles in the control (N; a-d, i-k, o-q, u-w) or mifepristoneinduced polycystic ovary model (PCO; e-h, l-n, r-t, x-z) mice on serial paraffin sections, prepared by in vivo cryotechnique, and stained with hematoxylin-eosin (HE, a, e) or immunostained for albumin (b, f, i-n), immunoglobulin G (IgG1, c, g, o-t) or IgM (d, h, u-z).…”

Section: Discussionmentioning

confidence: 51%

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Permselectivity of blood follicle barriers in mouse ovaries of the mifepristone-induced polycystic ovary model revealed by in vivo cryotechnique

Zhou

Ohno²,

Terada³

et al. 2008

Reproduction

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Despite the potential association of polycystic ovary (PCO) syndrome with hemodynamic changes, follicular microenvironment and the involvement of blood follicle barriers (BFB), a histopathological examination has been hampered by artifacts caused by conventional preparation methods. In this study, mouse ovaries of a mifepristone-induced PCO model were morphologically and immunohistochemically examined by in vivo cryotechnique (IVCT), which prevents those technical artifacts. Ovarian specimens of PCO model mice were prepared by IVCT or the conventional perfusion fixation after s.c. injection of mifepristone. Their histology and immunolocalization of plasma proteins, including albumin (molecular mass, 69 kDa), immunoglobulin G (IgG, 150 kDa), inter-a-trypsin inhibitor (ITI, 220 kDa), fibrinogen (340 kDa), and IgM (900 kDa), were examined. In the PCO model, enlarged blood vessels with abundant blood flow were observed in addition to cystic follicles with degenerative membrana granulosa. The immunolocalization of albumin and IgM in the PCO model were similar to those in normal mice. Albumin immunolocalized in the blood vessels, interstitium or follicles, and IgM was mostly restricted within the blood vessels. In contrast, immunolocalization of IgG, ITI, and fibrinogen changed in the PCO model. Both IgG and ITI were clearly blocked by follicular basement membranes, and hardly observed in the membrana granulosa, though fibrinogen was mostly observed within blood vessels. These findings suggest that increased blood flow and enhanced selectivity of molecular permeation through the BFB are prominent features in the PCO ovaries, and changes in hemodynamic conditions and permselectivity of BFB are involved in the pathogenesis and pathophysiology of PCO syndrome. Reproduction (2008) 136 599-610

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Section: Histological Examination Of Mouse Ovaries After Injection Ofsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 51%

Permselectivity of blood follicle barriers in mouse ovaries of the mifepristone-induced polycystic ovary model revealed by in vivo cryotechnique

Zhou

Ohno²,

Terada³

et al. 2008

Reproduction

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“…Microscopic examination of vaginal cytology has been used with confidence to monitor estrous cycles in a number of mammalian species [8,[11][12][13][14][15] and to examine the effects of hormones, drugs, toxins, contraceptives, and fertility agents on reproductive processes [16][17][18]. Behavioral, hormonal, and cytological changes associated with the estrous cycle of rats and mice have been rigorously examined over many years and are now well characterized [8,[11][12][13].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Effects of Human Follicle-Stimulating Hormone-Related Synthetic Peptide hFSH-,β-(81–95) and Its Subdomain hFSH-β-(90–95) on the Mouse Estrous Cycle

Grasso

Rozhavskaya

Reichert

1998

Biology of Reproduction

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We have previously reported that a synthetic peptide corresponding to amino acid residues 81-95 of the human (h) FSH-beta subunit inhibited binding of [125I]hFSH to bovine calf testis membranes and stimulated estradiol biosynthesis in primary cultures of rat Sertoli cells. We have now obtained several lines of evidence demonstrating in vivo effects of hFSH-beta-(81-95) on the mouse estrous cycle. 1) A single i.p. injection of 200 micrograms/g BW hFSH-beta-(81-95) significantly (p < 0.001) prolonged vaginal estrus in comparison to that in vehicle-injected control mice. 2) Vaginal smears taken at estrus from mice given hFSH-beta-(81-95) were characterized by the complete absence of epithelial casts, a hallmark of spontaneous ovulation in mice. 3) Mice receiving hFSH-beta-(81-95) had significantly (p < 0.001) lower serum estradiol at proestrus and serum progesterone at diestrus than vehicle-injected control mice. 4) The proestrous effects of estrogen on uterine ballooning and weight gain, clearly evident in vehicle-injected control mice, were not observed in mice treated with hFSH-beta-(81-95). A synthetic peptide corresponding to the carboxy-terminal region of hFSH-beta-(81-95), hFSH-beta-(90-95), inhibited binding of [125I]hFSH to bovine calf testis membranes, antagonized FSH-stimulated estradiol biosynthesis by primary cultures of rat Sertoli cells, and prolonged vaginal estrus in normally cycling mice. A synthetic peptide corresponding to the amino-terminal domain, hFSH-beta-(81-86), was inactive in vitro and had no effect on the mouse estrous cycle. The results of the present study provide additional evidence for in vivo effects of FSH-related synthetic peptides.

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“…The progesterone antagonist (which is difficult for the body to clear) can be fed monthly as a contraceptive and once in early pregnancy to interrupt pregnancy. It acts by causing a 163 sufficient disruption in the uterine lining to prevent implantation (Gao and Short 1994).…”

Section: Contragestion Technologymentioning

confidence: 99%

Induced fertility as a wildlife management tool

Miller¹,

Fagerstone²

2000

vertebrate_pest_conference

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A growing interest in nonlethal methods for population control of nuisance or damaging species of wildlife has fostered research in reducing fertility of these overabundant wildlife species. Fertility may be reduced by interfering with the fertilization of the egg (contraception) or interfering with the implantation or development of the fertilized egg (contragestion). Research using injectable porcine zona pellucida (PZP) and gonadotropin releasing hormone (GnRH) vaccines has demonstrated that several mammalian species can be contracepted, reducing fertility for several years without revaccination. However, because vaccines currently require delivery by syringe or bio-bullet, there is a need for infertility agents that can be delivered orally. Researchers are therefore considering materials that have resulted in reduced reproductive rates in the agricultural industry. Because of the cost of getting new technology approved by the FDA, materials already approved for other purposes that can be redirected for use in wildlife infertility may have a better chance of getting approved as wildlife infertility agents. Two compounds used in the broiler chicken industry have been found to reduce hatchability if given to the layer. Due to the rapid increase of Canada geese in our parks, research is ongoing with these two compounds to reduce hatchability in the Canada goose egg. Research is also underway to test a cholesterol mimic that competes with cholesterol as the parent compound for steroid synthesis. This compound could reduce fertility in both mammalian and avian species and is currently being tested in rodents. Natural plant materials such as phytoestrogens and ergot derivatives that result in reproductive losses in domestic animals should be also explored as reproductive inhibitors in overabundant species of wildlife.

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Fertility control in laboratory rats and mice after feeding with the antigestagen RU486

Cited by 20 publications

References 9 publications

Permselectivity of blood follicle barriers in mouse ovaries of the mifepristone-induced polycystic ovary model revealed by in vivo cryotechnique

Permselectivity of blood follicle barriers in mouse ovaries of the mifepristone-induced polycystic ovary model revealed by in vivo cryotechnique

In Vivo Effects of Human Follicle-Stimulating Hormone-Related Synthetic Peptide hFSH-,β-(81–95) and Its Subdomain hFSH-β-(90–95) on the Mouse Estrous Cycle

Induced fertility as a wildlife management tool

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