Fertility evaluation in Egyptian buffalo bulls using zona pellucida binding and in vitro fertilization assays

Dayem, A.M.H. Abdel; Mahmoud, Karima Gh. M.; Nawito, M.; Ayoub, Mohamed; Scholkamy, T. H.

doi:10.1016/j.livsci.2008.08.014

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…However, semen volume, percentage of acrosome integrity and abnormalities did not differ between good and poor. In accordance, many studies have recorded that bad sperm quality, leads to decrease the rate of blastocysts (20)(21)(22), and high rates of pregnancy failure (23). Moreover, (24) documented that fertility from the artificial insemination correlated with semen parameter.…”

Section: Discussionmentioning

confidence: 68%

GnRHR gene polymorphism and its correlation with semen quality in Buffalo bulls (Bubalus bubalis)

Mahmoud¹,

Sakr²,

Ibrahim³

et al. 2021

IJVS

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Fresh semen from fifty bulls was evaluated for ejaculate volume, individual motility, concentration, lives sperm and abnormalities as well as acrosome integrity. Bulls were classified according to semen motility into two groups; good and poor. DNA was extracted from semen of both groups, then the PCR followed by single-strand conformation polymorphism (SSCP) techniques were performed for mutation detection in gonadotropin releasing hormone receptor (GnRHR) gene through multiple sequence alignment. The results showed that the percentages of sperm motility, sperm concentration and live sperm, were significantly higher in good than poor semen quality bulls. However, semen volume, percentage of acrosome integrity and abnormalities did not differ between good and poor. The PCR amplification of 240-bp fragment and the results of SSCP appeared a genetic polymorphism with two patterns. Pattern I was seen in all good bulls, with incidence of 100%. Pattern II appeared only in poor semen quality bulls with a frequency of 31.25%. The sequence analysis of the PCR product for the two patterns showed two single nucleotide polymorphisms (SNPs) as a transversion base substitution mutation at positions 20 (T/A) and 193 (A/T). The GnRHR gene could be used as a genetic marker related to semen quality in buffalo due to the good semen bulls had a unique pattern.

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Section: Discussionmentioning

confidence: 68%

GnRHR gene polymorphism and its correlation with semen quality in Buffalo bulls (Bubalus bubalis)

Mahmoud¹,

Sakr²,

Ibrahim³

et al. 2021

IJVS

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“…Sperm HBA is a very simple, faster to perform and technically easier (Lazarevic et al., 2010) compared with sperm penetration assay (Park et al., 2012) and zona pellucida binding assay (Abdel Dayem et al., 2009) that were earlier attempted to predict fertilising ability of cattle bull and buffalo bull spermatozoa respectively. It is suggested to make this robust assay more cost effective for nonhuman use, and specifically for prediction of fertility in buffalo bulls as this animal has low conception rate with AI.…”

Section: Discussionmentioning

confidence: 99%

Sperm binding to hyaluronan is an excellent predictor of Nili‐Ravi buffalo bull fertility

et al. 2021

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This study reports the first evaluation of sperm hyaluronan binding assay (HBA) for predicting the fertility of Nili‐Ravi buffalo bulls in relation to standard parameters of sperm quality. Cryopreserved semen doses of low (n = 6), medium (n = 3) and high fertility (n = 8) bulls based on their respective return rates were used. Significantly, more spermatozoa bound to hyaluronan from the most fertile bulls (57.15% ± 1.44) compared with medium (42.46% ± 1.08) and low fertility bulls (29.70% ± 0.78). A strongly positive correlation (r = .824, p < .01) was found between HBA and fertility that predicts a 67.9% variability (r2 = .679, p < .01) in fertility. HBA was also strongly positively correlated with sperm viability (r = .679, p < .01) followed by their live/dead ratio (r = .637, p < .01), uncapacitated spermatozoa (r = .631, p < .01), normal apical ridge (r = .459, p < .01), motility (r = .434, p < .01), mature spermatozoa with low residual histones (r = .364, p < .01), high plasma membrane integrity (r = .316, p < .01) and nonfragmented DNA levels (r = .236, p < .05). It was negatively correlated with spermatozoa having reacted acrosome (r = −.654, p < .01). A fertility model built using a combination of sperm HBA and either sperm livability or viability predicts, respectively, 86.1% (r2 = .861, p < .01) and 85.9% (r2 = .859, p < .01) variability in buffalo bull fertility. In conclusion, sperm HBA may prove to be a single robust predictor of Nili‐Ravi buffalo bull fertility.

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A systematic review and meta-analysis of spermatozoa cryopreservation, in vitro and in vivo fertility practices in water buffalo

Shah

Andrabi²

2021

Vet Res Commun

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Fertility evaluation in Egyptian buffalo bulls using zona pellucida binding and in vitro fertilization assays

Cited by 4 publications

References 40 publications

GnRHR gene polymorphism and its correlation with semen quality in Buffalo bulls (Bubalus bubalis)

GnRHR gene polymorphism and its correlation with semen quality in Buffalo bulls (Bubalus bubalis)

Sperm binding to hyaluronan is an excellent predictor of Nili‐Ravi buffalo bull fertility

A systematic review and meta-analysis of spermatozoa cryopreservation, in vitro and in vivo fertility practices in water buffalo

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