Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soy bean extract

Leeuw, A.M. van Wagtendonk-de; Haring, R.M.; Kaal-Lansbergen, L.M.T.E.; Daas, J.H.G. den

doi:10.1016/s0093-691x(00)00324-1

Cited by 110 publications

(64 citation statements)

References 7 publications

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“…High soybean lecithin concentrations have been noted to be toxic for sperm motility and viability [5] . The impact of lecithin on sperm motility results from the extender viscosity and the presence of particulate debris [20] . Herein, there were no significant differences between the L1, L3 and L6 groups in terms of post-thaw motility (P>0.05).…”

Section: Discussionmentioning

confidence: 99%

Effect of Egg Yolk and Soybean Lecithin on Tris-Based Extender in Post-Thaw Ram Semen Quality and in vitro Fertility

Üstüner¹,

Alçay²,

Nur³

et al. 2014

Kafkas Univ Vet Fak Derg

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The aim of the current study was to evaluate the effect of egg yolk and different soybean lecithin concentrations on the efficiency of ram semen cryopreservation and to test the fertilizing ability of frozen-thawed ram semen. Ejaculates with a thick consistency, rapid wave motion (3-5), and >70% initial motility were pooled. Pooled semen were then divided into four groups and diluted at 1/5 (semen/extender) with 1%, 3%, 6% lecithin (L1, L3 and L6) or 20% egg yolk (EY20) using the two-step dilution method. As expected, the results of the current study showed that both motility and the rates of defective acrosomes in sperm were negatively affected by the cryopreservation procedure (P<0.001). The motility values of at 5°C and post-thawed semen in the EY20 group were significantly higher than those in the L1, L3 and L6 groups (P<0.05). There were no differences in motility rates among the lecithin groups at the dilution, cooling, equilibration or post thawing stages (P>0.05). The results of in vitro fertilization, as assessed by the rate of blastocyst formation, were more successful in the EY20 group than those noted in different lecithin groups. In conclusion, freezing ram semen with an extender containing egg yolk could yield better post-thaw sperm parameters and embryonic development compared to lecithin containing extenders. Keywords: Cryopreservation, Lecithin, Egg yolk, Ram semen, IVF Tris-Bazlı Sulandırıcılarda Yumurta Sarısı ve Soya Lesitininin Eritme Sonrası Koç Spermasının Kalitesi ve in vitro Fertilite Üzerine Etkisi ÖzetBu çalışmanın amacı, yumurta sarısı ve farklı lesitin konsantrasyonlarının koç spermasının dondurulabilirliği üzerine etkisini değerlendirmek ve dondurup çözdürülen koç spermasının fertilizasyon yeteneğini tespit etmektir. Kitle hareketi (3-5) ve >%70 motiliteye sahip ejakülatlar birleştirildi (pooling). Pooling yapılan sperma dört gruba bölündü ve %1, %3, %6 (L1, L3 ve L6) lesitin veya %20 yumurta sarısı (EY20) içeren sulandırıcılar ile 1/5 (sperma/sulandırıcı) oranında iki aşamalı sulandırma yöntemi kullanılarak sulandırıldı. Bu çalışmanın sonucunda; motilite ve akrozomal bozukluk oranlarının dondurma prosedüründen olumsuz yönde etkilendiği tespit edildi (P<0.001). EY20 grubunun 5°C'de ve eritme sonrası motilite değerleri L1, L3 ve L6 gruplarına göre yüksek bulundu (P<0.05). Sulandırma, soğutma, ekilibrasyon ve eritme sonrası aşamalarda motilite oranları bakımından lesitin grupları arasında farklılık saptanmadı (P>0.05). Blastosist oranları bakımından değerlendirilen in vitro fertilizasyon sonuçlarına göre, EY20 grubunun farklı lesitin gruplarından daha başarılı olduğu tespit edildi. Sonuç olarak, yumurta sarısı içeren sulandırıcı ile dondurulan koç spermasının lesitin gruplarına göre eritme sonrası spermatolojik özellikler ve embriyonik gelişim bakımından daha üstün olduğu tespit edildi.

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Section: Discussionmentioning

confidence: 99%

Effect of Egg Yolk and Soybean Lecithin on Tris-Based Extender in Post-Thaw Ram Semen Quality and in vitro Fertility

Üstüner¹,

Alçay²,

Nur³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…A recent work showed that choline phospholipids from egg yolk and soy beans are equally effective in preventing membrane damage during cryopreservation of stallion spermatozoa [23]. However, for bull sperm cryopreservation, the use of extenders containing phospholipids of vegetal origin may be less effective than use of egg yolk-based extenders [24][25][26][27].…”

Section: Discussionmentioning

confidence: 99%

Effects of cryopreservation on the motile sperm subpopulations in semen from Asturiana de los Valles bulls

et al. 2009

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The aim of this study was to identify different motile sperm subpopulations in ejaculates from an autochthonous bull breed (Bos taurus) and to determine possible modifications in these subpopulations resulting from cryopreservation. Ejaculates were collected and cryopreserved following a conventional protocol. The overall sperm motility and the kinematic parameters of individual spermatozoa were evaluated in fresh ejaculates, after 4 h at 5 8C, and at 0 and 2 h postthaw. A multivariate clustering procedure separated 23,585 motile spermatozoa into four subpopulations: Subpopulation 1 showed medium velocity (VCL: 99.4 AE 17.8 mm/sec) and high progressiveness (LIN: 65.1 AE 14.0%); Subpopulation 2 included spermatozoa with high velocity (VCL: 148.7 AE 25.6 mm/sec) but a nonprogressive trajectory (LIN: 33.1 AE 10.5%); Subpopulation 3 represented slowly motile (VCL: 58.3 AE 24.3 mm/sec) and nonprogressive sperm (LIN: 39.6 AE 18.3%); and Subpopulation 4 included very rapid (VCL: 152.8 AE 25.7 mm/sec) and highly progressive sperm (LIN: 70.9 AE 13.7%). Subpopulation 4 was present in the greatest quantity in fresh ejaculates (36%), but after cooling, it significantly decreased (21%) concomitantly with an increase (P < 0.001) in Subpopulation 2 (from 21% in fresh to 34% in postcooled semen). After freezing and thawing, the overall sperm motility was reduced, mainly due to Subpopulation 2 decreasing from 34% after cooling to 14% after thawing. Differences among bulls in the frequency distribution of spermatozoa within subpopulations were evidenced after thawing by different proportions of spermatozoa in Subpopulations 2 and 4. The current results indicate that a structure of four sperm subpopulations may be a common characteristic of bovine ejaculates and that the cooling phase of cryopreservation seems to be the determinant of postthaw semen quality. #

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“…Therefore, it seems that removal of chicken egg yolk from semen extenders would produce several advantages, such as improved consistency in the components of semen extenders and elimination of hygienic risks. For these reasons, it is necessary to develop a synthetic semen extender free of egg yolk [6][7][8][9]. Bovine serum albumin (BSA) is one of proteins available for replacement of egg yolk [11,12].…”

Section: Discussionmentioning

confidence: 99%

“…Howe ver, complete elimination of animal ingredients, including BSA, is desirable for production of a defined egg yolk-free semen extender. A soybean lecithin based extender (AndroMed, Minitube, Tiefenbach, Germany) has been developed and utilized for bovine and the Mountain gazelle semen [8][9][10]; its results in a higher non-return rate after AI compared with extender containing egg yolk [9]. As reported above, elimination of egg yolk from semen extender would make the whole process for semen cryopreservation and AI significantly better and safer.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, development of a chemically defined semen extender would be required in order to eliminate possible infectious origins from egg yolk added to the semen extender. Several investigations have been conducted for development of semen extenders that do not contain egg yolk, such as a defined extender containing soybean lecithin for bovine and wild gazelle semen [6][7][8][9][10], and bovine serum albumin (BSA) has also been used as a substitute of egg yolk for rainbow trout and turkey spermatozoa [11,12]. …”

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confidence: 99%

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Fertility of Ewes Inseminated Intrauterinally with Frozen Semen Using Extender Containing Bovine Serum Albumin

et al. 2007

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Abstract. The present study was conducted to examine the fertility of ewes inseminated intrauterinally with frozen semen using semen extender containing either egg yolk or bovine serum albumin (BSA). Sixty Suffolk and cross-bred ewes were treated with controlled internal drug release (CIDR) devices during the non-breeding season (July 2006). A CIDR was inserted into the vagina for 12 days and an intramuscular injection of 500 IU equine chorionic gonadotropin was administered one day before its removal. Ejaculates from a suffolk ram were diluted with a Tris-based extender containing either 15% (v/v) egg yolk or 10% (w/v) BSA, and the diluted semen was frozen in 0.25 ml straws. A fixed-time intrauterine artificial insemination (AI) was performed 43-47 h after CIDR removal, regardless of incidence of estrus. There was no significant difference in pregnancy rates at 60 days after AI between the extenders containing egg yolk (66.7%, 20/30 animals) or BSA (65.5%, 19/29 animals). Furthermore, there were no significant difference in the lambing rates (66.7% and 62.1%) and prolificacy (1.25 and 1.56) between the two semen extenders. The present study indicates that a semi-defined semen extender containing 10% BSA produces fertility after intrauterine AI that is similar to that achieved with semen extender containing egg yolk. o s t s e m e n e x t e n d e r s f o r f r e e z i n g o f spermatozoa, including ram semen contain egg yolk, milk, or a combination of the two as a basic ingredient. Although egg yolk is beneficial for sperm cryopreservation because it protects a g a i n s t c o l d s h o c k [ 1 ] , t h e r e a r e s e v e r a l disadvantages of addition of egg yolk to semen extender. Preparation of uniform semen extenders containing egg yolk is difficult because individual egg yolk quality may vary depending on the number of days after laying and the storage period. Furthermore, addition of egg yolk reduces the acrosome integrity of goat spermatozoa [2], and high egg yolk concentrations reduce the postthawing viability of ejaculated spermatozoa in several species, such as goats [3], rams [4] and water buffaloes [5]. Finally, development of a chemically defined semen extender would be required in order to eliminate possible infectious origins from egg yolk added to the semen extender. Several investigations have been conducted for development of semen extenders that do not contain egg yolk, such as a defined extender containing soybean lecithin for bovine and wild gazelle semen [6][7][8][9][10], and bovine serum albumin (BSA) has also been used as a substitute of egg yolk for rainbow trout and turkey spermatozoa [11,12].

show abstract

Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soy bean extract

Cited by 110 publications

References 7 publications

Effect of Egg Yolk and Soybean Lecithin on Tris-Based Extender in Post-Thaw Ram Semen Quality and in vitro Fertility

Effect of Egg Yolk and Soybean Lecithin on Tris-Based Extender in Post-Thaw Ram Semen Quality and in vitro Fertility

Effects of cryopreservation on the motile sperm subpopulations in semen from Asturiana de los Valles bulls

Fertility of Ewes Inseminated Intrauterinally with Frozen Semen Using Extender Containing Bovine Serum Albumin

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