Fertilization and early embryology: CD44 is expressed throughout pre-implantation human embryo development

Campbell, Suzanne; Swann, H.R.; Aplin, John D.; Seif, Mourad W.; Kimber, Sue; Elstein, Max

doi:10.1093/oxfordjournals.humrep.a135955

Cited by 113 publications

(68 citation statements)

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“…A role for HA in embryo adhesion is also suggested by the observation that tumors are highly enriched with HA and that their invasiveness is correlated with HA expression [18,19]. These data and the detection of HA receptors on mammalian embryos suggest that HA plays a role in an embryo attachment, implantation, and development after being transferred to the uterine cavity [3].…”

Section: Discussionmentioning

confidence: 90%

“…By binding to receptor CD44, HA may have cellmediated activity [3]. Interestingly, human embryos possess CD44 receptors and these can be detected until the blastocyst has formed [3].…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, human embryos possess CD44 receptors and these can be detected until the blastocyst has formed [3]. On the other hand, the human endometrium is receptive for implantation of an embryo only during a short secretory phase, the so-called implantation window.…”

Section: Introductionmentioning

confidence: 99%

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Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Nakagawa¹,

Takahashi²,

Nishi³

et al. 2012

J Assist Reprod Genet

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Purpose To ascertain whether the use of hyaluronanenriched transfer medium (HETM) improves pregnancy and implantation rates among embryo transfer patients with a history of multiple implantation failures. Methods Patients (n0314) under the age of 40 and with a history of multiple unsuccessful embryo transfers were enrolled. There were three groups of patients: those undergoing fresh embryo transfer (fresh ET [n 0111]), those undergoing vitrified-warmed ET in the natural cycle (WET-N [n0101]) and those undergoing WET in a hormone replacement cycle ). On the day of ET, patients were randomized to HETM (0.5 mg/ml hyaluronan) or control medium containing no hyaluronan. Only patients with good quality embryos on day 3 were included. Results For all three patients groups (fresh ET, WET-N and WET-H) pregnancy rates (37.5 %, 31.4 % and 41.2 %, respectively) were significantly higher when using HETM compared with control medium (10.9 %, 10.0 % and 15.7 %, respectively; p<0.05), and implantation rates when using HETM were also significantly higher compared with control medium (p<0.05). Miscarriage rates were similar in both groups. Conclusion HETM significantly increased pregnancy and implantation rates among embryo transfer patients with a history of multiple unsuccessful implantations-regardless of method used to prepare the endometrium.

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Section: Discussionmentioning

confidence: 90%

“…By binding to receptor CD44, HA may have cellmediated activity [3]. Interestingly, human embryos possess CD44 receptors and these can be detected until the blastocyst has formed [3].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Nakagawa¹,

Takahashi²,

Nishi³

et al. 2012

J Assist Reprod Genet

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“…CD44 is thought to play roles in cell motility and maintenance of cell shape, and is also involved in transformation and metastasis through ERM proteins [41]. Campbell et al [11] reported qualitative CD44 expression on human preimplantation embryos. Interestingly, we consistently found murine oocytes to express the highest levels of CD44, suggesting its possible involvement in maintaining the oocyte-cumulus-cell complex.…”

Section: Discussionmentioning

confidence: 99%

“…Expression of the HCAM, CD44, although reported on human embryos [11] has not been examined in the murine embryo. Its common ligand is the extracellular matrix molecule, hyaluronate, widely expressed throughout embryonic and adult tissues.…”

Section: Introductionmentioning

confidence: 99%

Regulation of cellular adhesion molecule expression in murine oocytes, peri-implantation and post-implantation embryos

Tian

O.'Neill

et al. 2002

Cell Res

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Expression of the adhesion molecules, ICAM-1, VCAM-1, NCAM, CD44, CD49d (VLA-4, α chain), and CD11a (LFA-1, α chain) on mouse oocytes, and pre-and peri-implantation stage embryos was examined by quantitative indirect immunofluorescence microscopy. ICAM-1 was most strongly expressed at the oocyte stage, gradually declining almost to undetectable levels by the expanded blastocyst stage. NCAM, also expressed maximally on the oocyte, declined to undetectable levels beyond the morula stage. On the other hand, CD44 declined from highest expression at the oocyte stage to show a second maximum at the compacted 8-cell/morula. This molecule exhibited high expression around contact areas between trophectoderm and zona pellucida during blastocyst hatching. CD49d was highly expressed in the oocyte, remained significantly expressed throughout and after blastocyst hatching was expressed on the polar trophectoderm. Like CD44, CD49d declined to undetectable levels at the blastocyst outgrowth stage. Expression of both VCAM-1 and CD11a was undetectable throughout. The diametrical temporal expression pattern of ICAM-1 and NCAM compared to CD44 and CD49d suggest that dynamic changes in expression of adhesion molecules may be important for interaction of the embryo with the maternal cellular environment as well as for continuing development and survival of the early embryo.

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The expression of genes in human preimplantation embryos

Pergament

Fiddler

1998

Prenat. Diagn.

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The study of gene expression in human preimplantation embryos is establishing itself as a necessary dimension of developmental biology and medical genetics. Transcripts identified in human preimplantation embryos include housekeeping genes, transcription and growth factor genes, sex‐determining genes, tissue‐specific genes and novel genes, as well as genes of unknown function. Strategies are being developed which will eventually permit the most sophisticated gene expression studies on single human embryos of co‐ordinated transcription and translational regulation. There is both a need for international co‐operation for the systematic construction of expression maps and a need to establish databases of expression patterns during different stages of human development. Understanding how genes are regulated in humans is essential for understanding both normal development and disease. Until recently, studies of gene expression and regulation during embryogenesis were almost exclusively limited to prokaryotes and to eukaryotes other than man. The introduction of artificial reproductive technologies in conjunction with the development of recombinant molecular technologies applicable to single cells has made possible the study of human development at its earliest stages (Pergament and Bonnicksen, 1994). Although there are still enormous technical challenges, robust strategies have been, and continue to be, developed for connecting DNA sequence to such endophenotypes as timing and level of gene expression at the single cell level. Questions currently being asked in human developmental genetic studies concern the pronucleus, the zygote and the preimplantation embryo: what genes are expressed? When are they expressed? What functions do they perform and how, in sequence or in combination? And, what elements control and regulate their expression? This review provides an overview of current knowledge about the expression of different embryonic genes during early human development and discusses future prospects, which includes a need for international co‐operation similar to the Human Genome Project. Copyright © 1998 John Wiley & Sons, Ltd.

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Fertilization and early embryology: CD44 is expressed throughout pre-implantation human embryo development

Cited by 113 publications

References 0 publications

Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Regulation of cellular adhesion molecule expression in murine oocytes, peri-implantation and post-implantation embryos

The expression of genes in human preimplantation embryos

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