Fertilization and pregnancy achieved by intracytoplasmic injection of sperm retrieved from testicular biopsies

Abuzeid, Mostafa I.; Chan, Y. M.; Sasy, Mohamed A.; Basata, Samir; Beer, Michael

doi:10.1016/s0015-0282(16)57807-8

Cited by 17 publications

(6 citation statements)

References 5 publications

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“…Several surgical sperm retrieval methods were introduced, this includes open surgery aimed at the testis by testicular biopsy called testicular sperm extraction (TESE) [Silber et al 1995a;Silber et al 1995b;Abuzeid et al 1995] or testicular sperm aspiration (TESA) [Craft et al 1997;Westlander et al 2001]. Similarly, procedures aimed at epididymis by micro-surgical epididymal sperm aspiration (MESA) were also developed [Tournaye et al 1994;Zenke et al 2004].…”

Section: Introductionmentioning

confidence: 99%

Impact of sperm retrieival on testis and epididymis: an experimental study using Wistar albino rats

Prithiviraj

Suresh

Manivannan

et al. 2013

Systems Biology in Reproductive Medicine

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The aim of this study was to analyze pathophysiological changes after testicular sperm aspiration (TESA) and microsurgical epididymal sperm aspiration (MESA) procedures. Twenty four mature male Wistar albino rats with a proven breeding history, weighing approximately 200-250 gm were used for the study. Animals were randomly divided into four groups (n = 6), i.e., control, sham-control, unilateral TESA, and MESA. Using a 22G needle, the aspiration procedures were done in testis or caudal epididymis. At the end of 60 days of survival, blood samples were collected and processed for antisperm antibody detection by enzyme-linked immunosorbent assay (ELISA). After euthanasia, testes and epididymides were collected and processed for paraffin embedding. Sections were stained with hematoxylin and eosin, and TUNEL technique. Serum antisperm antibody titer significantly increased in TESA (P < 0.001) when compared to MESA. Histomorphometric analysis indicated testicular alterations in TESA and MESA, with significant damage in TESA in both testes (P < 0.001). Following the MESA procedure, ipsilateral caudal and carpus epididymis showed significant alterations (P < 0.001) and no such alterations were seen in the ipsilateral caput and intact contralateral epididymis. TUNEL staining revealed an up-regulation of apoptosis in both contra- and ipsilateral testes of TESA. Needle prick had produced drastic and irreversible alterations in testis of TESA. Ensuing processes of immunological and inflammatory reaction had the potential to disrupt spermatogenesis and increase germ cell apoptosis. However, extrapolating conclusions from the experimental model to the clinic needs to be done cautiously.

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Section: Introductionmentioning

confidence: 99%

Impact of sperm retrieival on testis and epididymis: an experimental study using Wistar albino rats

Prithiviraj

Suresh

Manivannan

et al. 2013

Systems Biology in Reproductive Medicine

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“…The treatment of male infertility has been dramatically improved over the past decade by the introduction of intracytoplasmic sperm injection (ICSI) (Palermo et al ., 1992; Van Steirteghem et al ., 1993). For the management of severe cases of infertility (such as congenital absence of the vas deferens, extreme oligoasthenozoospermia, and obstructive and nonobstructive azoospermia), testicular sperm extraction (TESE) may be applied and successfully combined with ICSI (Abuzeid et al ., 1995; Gil‐Salom et al ., 1995; Tucker et al ., 1995). TESE includes mechanical and/or enzymatic isolation of testicular spermatozoa from (multiple) testicular biopsies (Salzbrunn et al ., 1996; Mulhall et al ., 1997; Tournaye et al ., 1997).…”

Section: Introductionmentioning

confidence: 99%

Effects of various cryopreservation media and freezing-thawing on the morphology of rat testicular biopsies

Ježek¹,

Schulze

Kalanj-Bognar³

et al. 2001

Andrologia

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Currently, testicular sperm extraction is successfully combined with intracytoplasmic sperm injection into the oocyte (ICSI). Several pieces of a testicular biopsy can be frozen and thawed until the ICSI attempt. In this study, the effects of freezing-thawing on the morphology of rat testicular biopsies stored in different cryopreservation media were analysed. Each cryopreservation medium contained glycerol and/or dimethyl sulfoxide (DMSO) as cryoprotectants. In general, both glycerol and DMSO, when applied at moderate concentrations (6-25%), preserved the structure of the seminiferous epithelium. The freezing-thawing procedure had no significant effect on tubular diameter; however, it caused a 'folding' of the lamina propria and notable damage to Sertoli cells, spermatogonia and spermatocytes. Round and elongated spermatids and spermatozoa displayed occasional nuclear damage, vacuolization, and shrinkage/swelling of the cytoplasm. However, the vast majority of these cells maintained their normal structure in nearly all the applied cryomedia. It is concluded that freezing-thawing of testicular biopsies, and the cryopreservation medium, have a significant impact on the structure of the seminiferous epithelium, particularly on its basal compartment.

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“…Our previous study [17] showed that the degree of oocyte denudation (complete or partial) did not affect the outcome of ICSI, but denuded oocytes with an incubation period of more than 2 hr resulted in better ICSI outcomes than with an incubation period less than 2 hr. Most TESE methods use gentle mincing of testicular tissue with sterile needles, thus ac hieving the mechanical isolation of spermatozoa [37][38][39]. We prepared a TESE samples by mincing testicular tissue with a surgical blade, not needles.…”

Section: Discussionmentioning

confidence: 99%

A New Sperm Preparation Method for Testicular Sperm Extraction - Intracytoplasmic Sperm Injection (TESE-ICSI) Cycles: Simple, Effective and Rapid Method

Park

Song

et al. 2002

J.Mamm.Ova.Res.

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Abstract:The aim of t his study was to recover spermatozoa easily from fresh or frozen-thawed testicular sperm extraction (TESE) samples. We simply minced and washed the TESE samples in medium. Spermatozoa were recovered and washed in the bottom of a PVP droplet with an injection pipette and injected into oocytes. In all cycles 100% of spermatozoa (motile and/or immotile) could be recovered. Injected oocytes were fertilized (100% per cycle and in 41.9% of oocytes) and fertilized oocytes were cleaved (100% per cycle; 38.5% of injected oocytes; 91.8% of fertilized oocytes) a f t e r I C S I w i t h f r e s h o r f r o z e n -t h a w e d T E S E spermatozoa. The rates of progression to the embryo stage (2-8 cell) and blastocyst formation and their quality were similar in both the fresh sample group and the frozen-thawed sample group. Both of embryo transfer (73.5%) and embryo-blastocyst transfer (26.5%) were performed successfully. Thirty-four cycles of ICSI with testicular spermatozoa resulted in 23.5% pregnancy rates (fresh sample group, 22.2%; frozen-thawed sample group, 24%) with both embryo transfer (8%) and embryoblastocyst transfer (66.7%). This new sperm preparation method is very simple, easy, effective and rapid for recovering spermatozoa from TESE samples for ICSI. Key words: Fresh or frozen-thawed TESE sample, Spermatozoa recovery, ICSI, Embryo and blastocyst, PregnancyThe introduction of intracytoplasmic sperm injection (ICSI) [1] was originally considered for male factor infertility when spermatozoa could be found in the ejaculate. The possibility of achieving pregnancy with even one available spermatozoon has precipitated the search for a method to obtain spermatozoa from testicular tissue or epididymal sites, when seminal azoospermia is a constant finding. In patients with o b s t r u c t i v e a n d n o n o b st r u c t i v e a z o o s p e r m i a , fertilization and pregnancy can be accomplished by means of a combination of intracytoplasmic sperm injection with epididymal spermatozoa retrieved by microsurgical epididymal sperm aspiration or testicular sperm extraction [2][3][4][5][6][7]. Although ICSI is an effective clinical treatment strategy for male infertility patients, new types of technical difficulties have arisen, because of the extremely small number of spermatozoa handled in this procedure. The recovery of spermatozoa from extremely low quality sperm samples or TESE tissues for use in the ICSI procedure is very difficult [6][7][8].The aim of this study was to recover the spermatozoa easily from fresh or frozen-thawed TESE samples in a 3% polyvinylpyrrolidone (PVP) droplet. Materials and Methods Female patientsWomen under the age of 35 (mean: 28.7; range: 25-35 years) and having many oocytes retrieved (>10) were recruited for this study at

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Fertilization and pregnancy achieved by intracytoplasmic injection of sperm retrieved from testicular biopsies

Cited by 17 publications

References 5 publications

Impact of sperm retrieival on testis and epididymis: an experimental study using Wistar albino rats

Impact of sperm retrieival on testis and epididymis: an experimental study using Wistar albino rats

Effects of various cryopreservation media and freezing-thawing on the morphology of rat testicular biopsies

A New Sperm Preparation Method for Testicular Sperm Extraction - Intracytoplasmic Sperm Injection (TESE-ICSI) Cycles: Simple, Effective and Rapid Method

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