Fertilization in Mammals

Florman, Harvey M.; Ducibella, Tom

doi:10.1016/b978-012515400-0/50007-5

Cited by 94 publications

(96 citation statements)

References 631 publications

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“…A common element of the ZP-initiated AR mechanism in eutherian mammals is involvement of the Gi protein, and PTX, which is an inhibitor of Gi protein function, is known to inhibit the ZP-initiated AR in mammalian sperm (Florman & Ducibella 2006). Interestingly, it has been reported that PTX does not inhibit the progesterone-induced AR of human and mouse sperm (Tesarik et al 1993, Murase & Roldan 1996.…”

Section: Acrosome Reaction In Japanese Quailmentioning

confidence: 99%

“…It is well known that the zona pellucida (ZP), which is an extracellular matrix surrounding mammalian oocytes, plays an important role during fertilization because it possesses receptor molecules responsible for relatively species-specific sperm-egg binding, induces the sperm AR, as well as participates in the prevention of polyspermy achieved by its structural changes (Florman & Ducibella 2006). In mammals, the ZP family of glycoproteins has been identified as playing a key role in these events (Wassarman 1999).…”

Section: Introductionmentioning

confidence: 99%

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Induction of sperm acrosome reaction by perivitelline membrane glycoprotein ZP1 in Japanese quail (Coturnix japonica)

Sasanami

Murata²,

Ohtsuki³

et al. 2007

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The extracellular matrix surrounding avian oocytes, called the perivitelline membrane (PL), consists of at least two major glycoproteins, ZP3 and ZP1. Our previous study using Japanese quail had demonstrated that the PL obtained from the preovulatory follicles was incubated in vitro with spermatozoa, and perforations were observed. This result indicated that the PL might contain a constituent that possesses activity to initiate the acrosome reaction (AR) in quail. In order to elaborate upon our previous findings, we evaluated the effects of ZP3 and ZP1 on the induction of sperm AR in Japanese quail. Ejaculated sperm were incubated with or without the purified PL glycoprotein, and their acrosome status was determined based on the presence or absence of the acrosome. Treatment of spermatozoa with increasing doses of the purified monomeric ZP1 led to a concentration-dependent stimulation of AR. The purified dimeric ZP1 had similar effect. Moreover, we found that the ZP1-induced AR was significantly blocked by the digestion of the PL protein with PNGaseF. In contrast, the addition of purified ZP3 failed to induce AR at any doses tested. These results indicate that N-linked glycans on ZP1 play an important role in triggering the AR in Japanese quail.

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Section: Acrosome Reaction In Japanese Quailmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induction of sperm acrosome reaction by perivitelline membrane glycoprotein ZP1 in Japanese quail (Coturnix japonica)

Sasanami

Murata²,

Ohtsuki³

et al. 2007

Reproduction

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show abstract

“…Previous in vitro studies have consistently pointed to the importance of HCO 3 Ϫ as a medium component that, in concert with Ca 2ϩ COMMENTARY and a cholesterol-binding element (usually BSA), is required for capacitation (1,2). One model of HCO 3 Ϫ action has emerged from recent studies.…”

mentioning

confidence: 99%

“…Fertilization typically occurs in the ampulla of the oviduct. Sperm must reach the vicinity of the oocyte; penetrate between the several thousand cumulus oophorus cells; contact and penetrate the oocyte extracellular matrix, or zona pellucida; and finally adhere to and fuse with the oocyte plasma membrane (2). Uncapacitated sperm carry out these tasks inefficiently and fail at various steps in the process.…”

mentioning

confidence: 99%