Fertoeibacter niger gen. nov., sp. nov. a novel alkaliphilic bacterium of the family Rhodobacteraceae

Abstract

Three Gram-stain-negative, non-motile, oxidase- and catalase-positive, rod-shaped, black, facultative phototrophic bacterial strains, RG-N-1aT, DMA-N-7a and RA-N-9 were isolated from the water sample from Lake Fertő/Neusiedler See (Hungary). Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the strains form a distinct linage within the family Rhodobacteraceae and their closest relatives are Tabrizicola piscis K13M18T (96.32%) followed by Cypionkella psychrotolerans PAMC 27389T (96.25%). The novel bacterial strains prefer alkaline environments and grow optimally at 23–33 °C in the presence of NaCl (1–2 w/v%). Bacteriochlorophyll a was detected. Cells contained exclusively ubiquinone Q-10. The major cellular fatty acids were C18 : 1ω7c, C19 : 1iso ω5c, C18 : 0 3-OH and C18 : 1ω7c 11-methyl. The polar lipid profile contains diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid and four unidentified lipids. The assembled draft genome of RG-N-1aT had 33 contigs with N50 values 315 027 nt, 96× genome coverage, total length of 4 326 551 bp and a DNA G+C content of 64.9%. Genome-based calculations (genome-to-genome distance and DNA G+C percentage) and pairwise amino acid identity (AAI <73.5%) indicate that RG-N-1aT represents a novel genus. RG-N-1aT (=DSM 108317T=NCAIM B.02647T) is suggested as the type strain of a novel genus and species in the family Rhodobacteraceae , for which the name Fertoeibacter niger gen. nov., sp. nov. is proposed.

Anianabacter salinae gen. nov., sp. nov. ASV31T, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern

“…Anaerobic growth was tested at 30 • C for 15 days on MA in an anaerobic chamber with GENbox anaer (bioMérieux, Marcy-l'Étoile, France). Growth at different temperatures (4,8,15,20,25,30,37 and 42 • C) was determined on MA after 3 to 15 days of incubation. Salt tolerance and requirement for growth were tested in MB supplemented with NaCl at final concentrations of 0, 0.5, 1.0, 2.0, 3.0, 5.0, 10.0, 15.0, 20.0 and 23.0 g/L.…”

“…This family is a large phenotypically, metabolically and ecologically diverse taxonomic group. Most members of the family originated from aquatic environments and many of them require sodium ions or combined salts for growth [4]. At the time of writing, the family Rhodobacteraceae consists of 227 validly published genera, including synonyms (https://lpsn.dsmz.de/family/rhodobacteraceae, accessed on 20 January 2022) which are divided into six different phylogenetic subgroups: Stappia, Amaricoccus, Paracoccus, Rhodobacter, Rhodovulum and Roseobacter [5].…”

Szabonella alba gen. nov., sp. nov., a motile alkaliphilic bacterium of the family Rhodobacteraceae isolated from a soda lake

“…Comparison of strain DMG-N-6 T with closely related genera in the family Rhodobacteraceae; data were taken from the literature Taxa: 1, strain DMG-N-6 T ; 2, Tabrizicola[5, 6, 22-26, 28, 45, 48, 52], 3, Cereibacter[5,6,29,30,[53][54][55][56]; 4, Cypionkella[6,31,32,57]; 5, Fertoeibacter[58]; 6, Rhodobacter[3,26,27,[59][60][61][62][63][64][65][66][67][68]; 7, Frigidibacter[69][70][71][72]; 8, Actibacterium[73][74][75][76][77][78]; 9, Paracoccus[3,5,79,80]. BChla, bacteriochlorophyll a; PC, phosphatidylcholine; GL, glycolipid; DPG, diphosphatidylglycerol; +, present; −, absent.…”