Ferulic acid (FA) protects human retinal pigment epithelial cells from H₂O₂‐induced oxidative injuries

Abstract

The aim of present study is to investigate whether Ferulic acid (FA), a natural polyphenol antioxidant, was able to protect ARPE‑19 cells from hydrogen peroxide (H2O2)‑induced damage, and elucidate the underlying mechanisms. Our results revealed that FA pre‐treatment for 24 hours can reverse cell loss of H2O2‐induced ARPE‐19 cells via the promotion of cell proliferation and prevention of apoptosis, as evidenced by 5‐ethynyl‐2′‐deoxyuridine (EdU) incorporation and terminal deoxynucleotidyl transferase‐mediated dUTP nick end‐labelling (TUNEL) assay, respectively. Moreover, the addition of FA (5 mM) can decrease Bax and cleaved caspase‐3 protein expression, but increase Bcl‐2 protein expression in ARPE‐19 cells. Furthermore, H2O2‐induced oxidative stress in ARPE‐19 cells was significantly alleviated by FA, illustrated by reduced levels of ROS and MDA. In addition, the attenuated antioxidant enzymes activities of (SOD, CAT and GPX) and GSH level were reversed almost to the normal base level by the pre‐addition of FA for 24 hours. In all assays, FA itself did not exert any effect on the change of the above parameters. These novel findings indicated that FA effectively protected human ARPE‐19 cells from H2O2‐induced oxidative damage through its pro‐proliferation, anti‐apoptosis and antioxidant activity, suggesting that FA has a therapeutic potential in the prevention and treatment of AMD.

MiR-599 Protects Cardiomyocytes against Oxidative Stress-Induced Pyroptosis

“…In fact, different concentrations of H 2 O 2 have different effects on cells. More than 200 μ M of hydrogen peroxide induces apoptosis in endothelial cells, smooth muscle cells, and epithelial cells [ 29 ] [ 30 , 31 ]. Tong et al reported that low level of H 2 O 2 significantly promoted endothelial cell proliferation, migration, and tube formation; the mechanism is related to Nox-derived ROS [ 32 ].…”

Ferulic Acid Alleviates Myocardial Ischemia Reperfusion Injury Via Upregulating AMPKα2 Expression-Mediated Ferroptosis Depression

“…FA protects epithelial cells from oxidative damage induced by H 2 O 2 . 11 It has been reported that FA improves myocardial I/R injury through succinate dehydrogenase-dependent antioxidant activity. 12 Moreover, FA also activates AMPK to produce an anti-inflammatory function.…”

Ferulic Acid Ameliorates Hepatic Inflammation and Fibrotic Liver Injury by Inhibiting PTP1B Activity and Subsequent Promoting AMPK Phosphorylation

“…Ferulic acid (3-methoxy-4-hydroxycinnamic acid, FA) is a phenolic acid widely distributed in abundant grains, vegetables and plants such as Angelicae Sinensis Radix , Ligusticum Chuanxiong Rhizoma and Cimicifuga racemose ( Oliveira et al, 2019 ). It also exhibits a broad spectrum of biological activities, including repairing mitochondrial dysfunction ( Luo et al, 2020 ), anti-oxidant ( Xie et al, 2020 ), enhancement of immune function ( Kohno et al, 2020 ) and preventing epithelial-mesenchymal transition ( Ali et al, 2021 ). Several recent studies further provided evidence for the hepatoprotective effects of FA.…”