Fetal HLA typing in β thalassaemia: implications for haemopoietic stem-cell transplantation

Orofino, Maria Grazia; Argiolu, F; Sanna, Maria Luisa; Rosatelli, C; Tuveri, T; Scalas, M T; Badiali, Manuela; Cossu, P; Puddu, R.; Lai, Maria Eliana; Cao, Antonio

doi:10.1016/s0140-6736(03)13806-8

Cited by 25 publications

(16 citation statements)

References 4 publications

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“…140,141 For couples who have already had a child with thalassemia and who undertake prenatal diagnosis in a subsequent pregnancy, prenatal identification of HLA compatibility between the affected child and an unaffected fetus allows collection of placental blood at delivery and the option of cord blood transplantation to cure the affected child. 142 On the other hand, in case of an affected fetus and a previous normal child, the couple may decide to continue the pregnancy and pursue BMT later, using the normal child as the donor.…”

Section: Bone Marrow Transplantationmentioning

confidence: 99%

Beta-thalassemia

Cao

Galanello

2010

Genetics in Medicine

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560

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Section: Bone Marrow Transplantationmentioning

confidence: 99%

Beta-thalassemia

Cao

Galanello

2010

Genetics in Medicine

Self Cite

713

560

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“…In cases with a previous nonaffected child, we propose HLA typing on fetal DNA, to check whether the sibling could be HLA identical and therefore a suitable bone marrow donor (Orofino et al 2003). This information could be useful for deciding on noninterruption of the pregnancy in the case of an affected fetus.…”

Section: Genetic Counselingmentioning

confidence: 99%

The Prevention of Thalassemia

Cao

Kan

2013

Cold Spring Harbor Perspectives in Medicine

208

157

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The thalassemias are among the most common inherited diseases worldwide, affecting individuals originating from the Mediterranean area, Middle East, Transcaucasia, Central Asia, Indian subcontinent, and Southeast Asia. As the diseases require long-term care, prevention of the homozygous state constitutes a major armament in the management. This article discusses the major prevention programs that are set up in many countries in Europe, Asia, and Australia, often drawing from the experience in Sardinia. These comprehensive programs involve carrier detections, molecular diagnostics, genetic counseling, and prenatal diagnosis. Variability of clinical severity can be attributable to interactions with a-thalassemia and mutations that increase fetal productions. Special methods taht are currently quite expensive and not widely applicable are preimplantation and preconception diagnosis. The recent successful studies of fetal DNA in maternal plasma may allow future prenatal diagnosis that is noninvasive for the fetus.

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“…H ematopoietic stem cell (HSC) transplantation can compensate for tissue damage elicited by a wide variety of disorders, including malignant and͞or inherited diseases (1)(2)(3)(4)(5). However, broader clinical application is still limited because of a number of biological and technical problems.…”

mentioning

confidence: 99%

Multiorgan engraftment and differentiation of human cord blood CD34 ⁺ Lin ⁻ cells in goats assessed by gene expression profiling

Zeng

Chen

Baldwin

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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To investigate multitissue engraftment of human primitive hematopoietic cells and their differentiation in goats, human CD34 ؉ Lin ؊ cord blood cells transduced with a GFP vector were transplanted into fetal goats at 45-55 days of gestation. GFP ؉ cells were detected in hematopoietic and nonhematopoietic organs including blood, bone marrow, spleen, liver, kidney, muscle, lung, and heart of the recipient goats (1.2-36% of all cells examined). We identified human ␤2 microglobulin-positive cells in multiple tissues. GFP ؉ cells sorted from the perfused liver of a transplant goat showed human insulin-like growth factor 1 gene sequences, indicating that the engrafted GFP ؉ cells were of human origin. A substantial fraction of cells engrafted in goat livers expressed the human hepatocyte-specific antigen, proliferating cell nuclear antigen, albumin, hepatocyte nuclear factor, and GFP. DNA content analysis showed no evidence for cellular fusion. Long-term engraftment of GFP ؉ cells could be detected in the blood of goats for up to 2 yr. Microarray analysis indicated that human genes from a variety of functional categories were expressed in chimeric livers and blood. The human͞goat xenotransplant model provides a unique system to study the kinetics of hematopoietic stem cell engraftment, gene expression, and possible stem cell plasticity under noninjured conditions. hematopoietic stem cell ͉ transplantation ͉ plasticity ͉ microarray

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Fetal HLA typing in β thalassaemia: implications for haemopoietic stem-cell transplantation

Cited by 25 publications

References 4 publications

Beta-thalassemia

Beta-thalassemia

The Prevention of Thalassemia

Multiorgan engraftment and differentiation of human cord blood CD34 ⁺ Lin ⁻ cells in goats assessed by gene expression profiling

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Fetal HLA typing in β thalassaemia: implications for haemopoietic stem-cell transplantation

Cited by 25 publications

References 4 publications

Beta-thalassemia

Beta-thalassemia

The Prevention of Thalassemia

Multiorgan engraftment and differentiation of human cord blood CD34 + Lin − cells in goats assessed by gene expression profiling

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Multiorgan engraftment and differentiation of human cord blood CD34 ⁺ Lin ⁻ cells in goats assessed by gene expression profiling