Fetal myelomeningocele repair based on cell sheet technology.

Ishii, Rie; Sobhan, Ubaidus; Hebiguchi, Tatsuzo; Sato, Tomoo; Yamashita, Hiromasa; Tabata, Yasuhiko; Sawa, Yoshiki; Chiba, Tadatoshi; Chiku, Kazuo

doi:10.11638/jssmn.48.6_215

Cited by 1 publication

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“…Among diverse regenerative technologies, we used animal models of fetal MMC to focus on surgically transplanting a cell sheet onto the MMC lesion with potential full recovery of the fetal spinal cord architecture before birth. We previously found that such cell sheets could be biologically implanted and incorporated quite early after surgical application onto the fetal MMC 2 . In this article, we present the outcomes of our experimental study and the future prospects of cell sheet technology in the field of prenatal medicine.…”

Section: Introductionmentioning

confidence: 99%

A New Regenerative Approach to Fetal Myelomeningocele by Cell Sheet Transplantation

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Irie

2017

Showa Univ J Med Sci

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: Myelomeningocele MMC is the most common form of congenital neural tube defect. Current fetal surgical repair performed to prevent the exposed spinal cord from being injured until delivery cannot reverse those injuries already in icted in utero.Cell sheet technology has been adopted successfully for the regeneration of diverse organs and tissues, although this promising modality has not yet been used for fetal therapy. This study thus tested our hypothesis that fetal MMC tissue histologically injured in utero could be regenerated using cell sheet technology. We used the L6 myoblast cell line derived from rat skeletal muscle for the cell sheet engineering. A fetal MMC model was also obtained from pregnant Sprague-Dawley SD rats fed orally with retinoic acid 60 mg / kg, embryonic day 10 . Cell sheets were then transplanted onto the fetal MMC lesion embryonic day 19 following maternal anesthesia, laparotomy, and hysterotomy. The incisional wound of the uterus was kept open for 4 hours under anesthesia with the MMC lesion maintained outside the body with the transplanted cell sheet. Subsequently, the experimental fetuses were sacri ced for histological HE stain and immunohistochemical studies to evaluate viability and differentiation potential based on cell markers of the transplanted L6 myoblasts. Immunohistochemical studies clearly demonstrated cell-sheet markers speci c to neurons, skeletal muscle, and myoblasts within the treated MMC lesions, confirming that the cell sheet was biologically implanted within 4 hours after the procedure. Cell sheet technology could be useful for intrauterine regeneration of the fetal rat spinal cord injured by an associated MMC.

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Section: Introductionmentioning

confidence: 99%