Fgf22 regulated by Fgf3/Fgf8 signaling is required for zebrafish midbrain development

Miyake, Ayumi; Itoh, Nobuyuki

doi:10.1242/bio.20134226

Cited by 17 publications

(13 citation statements)

References 68 publications

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“…The gross morphological phenotypes obtained by an injection of either fgf16 MO1 or fgf16 MO2 were similar to each other (MO1, n = 78/89 and MO2, n = 79/112). On the other hand, control MO-injected embryos developed normally during embryogenesis [9] . Furthermore, the phenotype was confirmed by RNA rescue experiments.…”

Section: Resultsmentioning

confidence: 96%

“…The inhibition of fgf16 led to abnormalities in the regionalization and generation of specific cell types such as GABAergic interneurons and oligodendrocytes in the forebrain. Hh signaling is critical for regulating the expression of fgf3, fgf8 , and fgf19 in the forebrain and that of fgf19 and fgf22 in the midbrain [8] , [9] . Therefore, we examined whether the expression of Fgf16 was responsive to Hh signaling.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, Hedgehog (Hh) signaling is known to be critical for ventral patterning in the forebrain and midbrain [3] – [5] . Fibroblast growth factor (Fgf) signaling has also been implicated in dorsoventral patterning and the regulation of cell proliferation and differentiation in various regions during brain development [1] , [6] – [9] .…”

Section: Introductionmentioning

confidence: 99%

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Fgf16 Is Required for Specification of GABAergic Neurons and Oligodendrocytes in the Zebrafish Forebrain

et al. 2014

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Fibroblast growth factor (Fgf) signaling plays crucial roles in various developmental processes including those in the brain. We examined the role of Fgf16 in the formation of the zebrafish brain. The knockdown of fgf16 decreased cell proliferation in the forebrain and midbrain. fgf16 was also essential for development of the ventral telencephalon and diencephalon, whereas fgf16 was not required for dorsoventral patterning in the midbrain. fgf16 was additionally required for the specification and differentiation of γ–aminobutyric acid (GABA)ergic interneurons and oligodendrocytes, but not for those of glutamatergic neurons in the forebrain. Cross talk between Fgf and Hedgehog (Hh) signaling was critical for the specification of GABAergic interneurons and oligodendrocytes. The expression of fgf16 in the forebrain was down-regulated by the inhibition of Hh and Fgf19 signaling, but not by that of Fgf3/Fgf8 signaling. The fgf16 morphant phenotype was similar to that of the fgf19 morphant and embryos blocked Hh signaling. The results of the present study indicate that Fgf16 signaling, which is regulated by the downstream pathways of Hh-Fgf19 in the forebrain, is involved in forebrain development.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fgf16 Is Required for Specification of GABAergic Neurons and Oligodendrocytes in the Zebrafish Forebrain

et al. 2014

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“…Wnt1 has recently been proposed to mediate the timing of neurogenesis in the midbrain by driving Fgf8 expression at the boundary and gradually suppressing it away from the boundary by inducing Sprouty expression so that Fgf dependent her5 also recedes (Dyer et al, 2014 ). Wnt1 may also function to promote neural stem cell identity in the dorsal midbrain and MHB (Miyake et al, 2012 ; Lin and Lee, 2016 ), possibly regulated by Fgf3/8-dependent Fgf22 signaling (Miyake and Itoh, 2013 ) and may contribute to shaping the MH by regulating the cytoskeleton during axon guidance (Ciani and Salinas, 2005 ). In the hindbrain, differentiation of unique tegmentum nuclei identities happens in spatiotemporal waves emanating from the upper rhombic lip.…”

Section: Midbrain Hindbrain Domain Morphogenesis and Patterningmentioning

confidence: 99%

Midbrain-Hindbrain Boundary Morphogenesis: At the Intersection of Wnt and Fgf Signaling

et al. 2017

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A constriction in the neural tube at the junction of the midbrain and hindbrain is a conserved feature of vertebrate embryos. The constriction is a defining feature of the midbrain-hindbrain boundary (MHB), a signaling center that patterns the adjacent midbrain and rostral hindbrain and forms at the junction of two gene expression domains in the early neural plate: an anterior otx2/wnt1 positive domain and a posterior gbx/fgf8 positive domain. otx2 and gbx genes encode mutually repressive transcription factors that create a lineage restriction boundary at their expression interface. Wnt and Fgf genes form a mutually dependent feedback system that maintains their expression domains on the otx2 or gbx side of the boundary, respectively. Constriction morphogenesis occurs after these conserved gene expression domains are established and while their mutual interactions maintain their expression pattern; consequently, mutant studies in zebrafish have led to the suggestion that constriction morphogenesis should be considered a unique phase of MHB development. We analyzed MHB morphogenesis in fgf8 loss of function zebrafish embryos using a reporter driven by the conserved wnt1 enhancer to visualize anterior boundary cells. We found that fgf8 loss of function results in a re-activation of wnt1 reporter expression posterior to the boundary simultaneous with an inactivation of the wnt1 reporter in the anterior boundary cells, and that these events correlate with relaxation of the boundary constriction. In consideration of other results that correlate the boundary constriction with Wnt and Fgf expression, we propose that the maintenance of an active Wnt-Fgf feedback loop is a key factor in driving the morphogenesis of the MHB constriction.

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“…To test this, we examined 17 genes known to be important for the embryonic development of the optic tectum in vertebrates and looked up their evolutionary stages of appearance. For example, Otx2 , Fgf8 , En1/2 , and Wnt1 are important for early differentiation of the midbrain, whereas Pax3 and Pax7 specify the optic tectum ( Wurst and Bally-Cuif 2001 ; Agoston et al 2012 ; Miyake and Itoh 2013 ; Dyer et al 2014 ). We found that all 17 genes evolved at much earlier stages (ps2, ps5, and ps6), thus supporting our hypothesis of tectal evolution through co-option of old genes ( supplementary fig.…”

Section: Discussionmentioning

confidence: 99%

Phylostratigraphic Profiles in Zebrafish Uncover Chordate Origins of the Vertebrate Brain

Šestak

Domazet-Lošo

2014

Mol Biol Evol

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An elaborated tripartite brain is considered one of the important innovations of vertebrates. Other extant chordate groups have a more basic brain organization. For instance, cephalochordates possess a relatively simple brain possibly homologous to the vertebrate forebrain and hindbrain, whereas tunicates display the tripartite organization, but without the specialized brain centers. The difference in anatomical complexity is even more pronounced if one compares chordates with other deuterostomes that have only a diffuse nerve net or alternatively a rather simple central nervous system. To gain a new perspective on the evolutionary roots of the complex vertebrate brain, we made here a phylostratigraphic analysis of gene expression patterns in the developing zebrafish (Danio rerio). The recovered adaptive landscape revealed three important periods in the evolutionary history of the zebrafish brain. The oldest period corresponds to preadaptive events in the first metazoans and the emergence of the nervous system at the metazoan–eumetazoan transition. The origin of chordates marks the next phase, where we found the overall strongest adaptive imprint in almost all analyzed brain regions. This finding supports the idea that the vertebrate brain evolved independently of the brains within the protostome lineage. Finally, at the origin of vertebrates we detected a pronounced signal coming from the dorsal telencephalon, in agreement with classical theories that consider this part of the cerebrum a genuine vertebrate innovation. Taken together, these results reveal a stepwise adaptive history of the vertebrate brain where most of its extant organization was already present in the chordate ancestor.

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Fgf22 regulated by Fgf3/Fgf8 signaling is required for zebrafish midbrain development

Cited by 17 publications

References 68 publications

Fgf16 Is Required for Specification of GABAergic Neurons and Oligodendrocytes in the Zebrafish Forebrain

Fgf16 Is Required for Specification of GABAergic Neurons and Oligodendrocytes in the Zebrafish Forebrain

Midbrain-Hindbrain Boundary Morphogenesis: At the Intersection of Wnt and Fgf Signaling

Phylostratigraphic Profiles in Zebrafish Uncover Chordate Origins of the Vertebrate Brain

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