FgVELB is associated with vegetative differentiation, secondary metabolism and virulence in Fusarium graminearum

Jiang, Jinhua; Yun, Yingzi; Liu, Ye; Ma, Zhonghua

doi:10.1016/j.fgb.2012.06.005

Cited by 75 publications

(65 citation statements)

References 46 publications

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“…In A. nidulans , the velB deletion mutant showed decreased sterigmatocystin (SM) production and synthesized a brownish pigment6. In F. graminearum , the FgVelB mutant produced a yellow pigment and a dramatically low level of DON14. In the current study, we observed that ΔClVelB produced a significantly high level of melanin (Fig.…”

Section: Discussionsupporting

confidence: 50%

“…3B). In F. graminearum , the disruption of velB caused the hydrophobicity change of the cell surface14. Instead, we found that the clvelB deletion mutant exhibited no effect on hydrophobicity (Fig.…”

Section: Discussionmentioning

confidence: 76%

“…In A. nidulans , the disruption of either velB or veA results in defects in the SM production and sexual fruiting body formation22. In the same way, FgVelB and FgVeA have similar roles in regulating fungal development, glycerol accumulation, DON synthesis, and pathogenicity14, which indicates that VelB cooperates with VeA to regulate fungal development and secondary metabolism. In A. nidulans , VelB contains neither a typical nuclear export signal (NES) nor a nuclear localization signal (NLS).…”

Section: Discussionmentioning

confidence: 95%

“…The deletion of fgvelB leads to loss of function to maintain the hydrophobicity of the hyphal surface in Fusarium graminearum 14. To confirm if clvelB has the same function in C. lunata , 20 μl drops of 2.5% bromophenol blue solution or ddH 2 O were added to each strain surface.…”

Section: Resultsmentioning

confidence: 99%

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Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata

Gao

Wang

et al. 2017

Sci Rep

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The ortholog of Aspergillus nidulans VelB, which is known as ClVelB, was studied to gain a broader insight into the functions of a velvet protein in Curvularia lunata. With the expected common and specific functions of ClVelB, the deletion of clvelB results in similar though not identical phenotypes. The pathogenicity assays revealed that ΔClVelB was impaired in colonizing the host tissue, which corresponds to the finding that ClVelB controls the production of conidia and the methyl 5-(hydroxymethyl) furan-2-carboxylate toxin in C. lunata. However, the deletion of clvelB led to the increase in aerial hyphae and melanin formation. In addition, ΔClVelB showed a decreased sensitivity to iprodione and fludioxonil fungicides and a decreased resistance to cell wall-damaging agents and osmotic stress and tolerance to H2O2. The ultrastructural analysis indicated that the cell wall of ΔClVelB became thinner, which agrees with the finding that the accumulated level of glycerol in ΔClVelB is lower than the wild-type. Furthermore, the interaction of ClVelB with ClVeA and ClVosA was identified in the present research through the yeast two-hybrid and bimolecular fluorescence complementation assays. Results indicate that ClVelB plays a vital role in the regulation of various cellular processes in C. lunata.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

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Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata

Gao

Wang

et al. 2017

Sci Rep

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“…The transcription factor AreA, which is a global nitrogen regulator in filamentous fungi (Yin and Keller, 2011), is essential for DON production . The velvet complex, which is conserved throughout various genera of filamentous fungi as a regulator of development and metabolism, is also a positive regulator of DON production in F. graminearum (Jiang et al, 2012(Jiang et al, , 2011aKim et al, 2013;Lee et al, 2012;Merhej et al, 2012). Chromatin modifications mediated by HDF1…”

Section: Fusariummentioning

confidence: 99%

Regulation and cell biology of secondary metabolite production in Fusarium graminearum and Fusarium pseudograminearum

Blum¹

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1 AbstractAscomycete fungi have the potential to produce a vast array of secondary metabolites, which are metabolites not required for normal growth and development. In any one species there is typically the potential for production of upward of fifty of these compounds. Many fungal secondary metabolites are used as medicines (e.g. penicillin or cyclosporine), or are of importance due to their toxicity in humans or animals (e.g. aflatoxins), or because they are virulence factors during crop plant infection (e.g. deoxynivalenol). Despite the vast potential encoded in ascomycete genomes, the vast majority of fungal secondary metabolites are yet to be discovered. This is in part due to our lack of understanding of how the production of these compounds is regulated. Moreover, even for the compounds we do know, we are just beginning to understand the complex interaction of external stimuli, signalling pathways, cellular and developmental biology that leads to their production. In this thesis, biological insights into these aspects of two fungal secondary metabolites, deoxynivalenol (DON) and fusatin are provided.In chapter II a high-throughput fluorescence activated cell sorting (FACS) based mutant screen was developed and the regulation of DON production in Fusarium graminearum was analysed. DON is an economically very important mycotoxin, as it is the most common contaminant of wheat, barley and corn worldwide. It is produced by plant pathogenic filamentous fungi of the Fusarium family, which can cause Fusarium head blight as well as Fusarium crown rot. During plant infection, DON is an important virulence factor and it can be toxic for humans and animals. The regulation of DON production is not yet fully understood. To identify regulators, a mutant screen was developed using FACS to allow high throughput screening, coupled with whole genome sequencing to identify mutations.Segregation analyses were performed to determine the mutation causing the phenotype.The mutant screen identified an adenylyl cyclase allele, which resulted in production of DON under normally repressive conditions. In addition, the mutant developed cellular structures associated with toxin production under repressive conditions. The levels of the fundamental second messenger cAMP, which adenylyl cyclases produce, were increased in the identified mutant, suggesting it might be a gain-of-function adenylyl cyclase mutant. This mutant screening methodology can be adapted to identify regulators of different secondary metabolites as well as to identify mutants overproducing yet unknown secondary metabolites. Abstract 2In chapter III the cell biology of DON production in Fusarium graminearum was elucidated with quantitative super resolution microscopy. During the last five years the first insights into the cell biology of DON production were gained, indicating complex cell biological mechanisms. During toxin production, F. graminearum develops endoplasmic reticulum proliferations, called toxisomes, at which two DON biosynthesis enzymes, TRI1 a...

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Mycotoxins Occurrence, Toxicity and Detection Methods

Gacem

Hadj-Khelil

Boudjemaa

et al. 2020

Sustainable Agriculture Reviews

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FgVELB is associated with vegetative differentiation, secondary metabolism and virulence in Fusarium graminearum

Cited by 75 publications

References 46 publications

Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata

Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata

Regulation and cell biology of secondary metabolite production in Fusarium graminearum and Fusarium pseudograminearum

Mycotoxins Occurrence, Toxicity and Detection Methods

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