Fiber diameter and texture of electrospun PEOT/PBT scaffolds influence human mesenchymal stem cell proliferation and morphology, and the release of incorporated compounds

Moroni, Lorenzo; Licht, Ruud; Boer, Jan de; Wijn, J.R. de; Blitterswijk, Clemens A. van

doi:10.1016/j.biomaterials.2006.05.027

Cited by 237 publications

(214 citation statements)

References 50 publications

(67 reference statements)

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“…The high protein release from scaffolds of 100 mg/ml PRGF over the other scaffolds may be explained by the scaffold's smaller fiber diameters. Although not specifically investigated in this study, other previously published studies have demonstrated similar results, and explain that with smaller fiber diameters there is less distance for molecules to traverse to reach the fiber surface, hence, more protein release from the fibers over time [46][47][48][49]. The rise in protein release after day 21 may be due to fiber degradation of the PRGF scaffolds occurring around 28-35 days and subsequent release of entrapped proteins.…”

Section: Protein Release Kineticssupporting

confidence: 79%

The Creation of Electrospun Nanofibers from Platelet Rich Plasma

Wolfe¹,

Sell²,

Ericksen³

et al. 2011

J Tissue Sci Eng

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Activated platelet rich plasma (a PRP) contains supra physiologic amounts of autologous growth factors and cytokines known to enhance wound healing and tissue regeneration. Here we report the first results of electro spinning nanofibers from a PRP to create fibrous scaffolds that could be used for various tissue engineering applications. Human platelet rich plasma (PRP) was created, activated by a freeze-thaw-freeze process, and lyophilized to form a powdered preparation rich in growth factors (PRGF). It was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) at different concentrations to form fibers with average diameters of 0.3 -3.6 µm. A sustained release of protein from the PRGF scaffolds was demonstrated up to 35 days, and cell interactions with the PRGF scaffolds confirmed cell infiltration after just 3 days. As electro spinning is a simple process, and PRGF contains naturally occurring growth factors in physiologic ratios, creating nanofibrous structures from PRGF has the potential to be beneficial for a variety of tissue engineering applications.

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Section: Protein Release Kineticssupporting

confidence: 79%

The Creation of Electrospun Nanofibers from Platelet Rich Plasma

Wolfe¹,

Sell²,

Ericksen³

et al. 2011

J Tissue Sci Eng

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show abstract

“…the fraction of white pixels) in an automated fashion. Unlike the previous two methods [19,27,36], the third is not a standard procedure, but was used to check how porosity measurements obtained by the previous two methods correlated with the projected porosity. Statistical comparison of the three methods was carried out on average estimates from five measurements using the paired t-test at the 10% significance level (i.e.…”

Section: Porosity Measurementsmentioning

confidence: 99%

Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

et al. 2010

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a b s t r a c tA novel (scalable) electrospinning process was developed to fabricate bio-inspired multiscale threedimensional scaffolds endowed with a controlled multimodal distribution of fiber diameters and geared towards soft tissue engineering. The resulting materials finely mingle nano-and microscale fibers together, rather than simply juxtaposing them, as is commonly found in the literature. A detailed proof of concept study was conducted on a simpler bimodal poly(e-caprolactone) (PCL) scaffold with modes of fiber distribution at 600 nm and 3.3 lm. Three conventional unimodal scaffolds with mean diameters of 300 nm and 2.6 and 5.2 lm, respectively, were used as controls to evaluate the new materials. Characterization of the microstructure (i.e. porosity, fiber distribution and pore structure) and mechanical properties (i.e. stiffness, strength and failure mode) indicated that the multimodal scaffold had superior mechanical properties (Young's modulus $40 MPa and strength $1 MPa) in comparison with the controls, despite the large porosity ($90% on average). A biological assessment was conducted with bone marrow stromal cell type (mesenchymal stem cells, mTERT-MSCs). While the new material compared favorably with the controls with respect to cell viability (on the outer surface), it outperformed them in terms of cell colonization within the scaffold. The latter result, which could neither be practically achieved in the controls nor expected based on current models of pore size distribution, demonstrated the greater openness of the pore structure of the bimodal material, which remarkably did not come at the expense of its mechanical properties. Furthermore, nanofibers were seen to form a nanoweb bridging across neighboring microfibers, which boosted cell motility and survival. Lastly, standard adipogenic and osteogenic differentiation tests served to demonstrate that the new scaffold did not hinder the multilineage potential of stem cells.

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“…A variety of polymers such as PLGA, PLLA, PCL, PEOT-PBT, collagen, chitosan, or the composite materials [8,15,[18][19][20][21] have been successfully electrospun into micro/nanosize fibres. Depending on the materials used and spinning conditions, fibrous meshes with microscale variation, for instance, in pore size and fibre diameter [22], can be produced.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional nanofibrous scaffold for tissue engineering

Yang

Ogbolu

Wang

2008

Journal of Experimental Nanoscience

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In tissue engineering, scaffolds with multiscale functionality, especially with the ability to release locally multiple or specific bioactive molecules to targeted cell types, are highly desired in regulating appropriate cell phenotypes. In this study, poly (epsilon-caprolactone) (PCL) solutions (8% w/v) containing different amounts of bovine serum albumin (BSA) with or without collagen were electrospun into nanofibres. As verified by protein release assay and fluorescent labelling, BSA and collagen were successfully incorporated into electrospun nanofibres. The biological activity of functionalised fibres was proven in the cell culture experiments using human dermal fibroblasts. By controlling the sequential deposition and fibre alignment, 3D scaffolds with spatial distribution of collagen or BSA were assembled using fluorescently labelled nanofibres. Human dermal fibroblasts showed preferential adhesion to PCL nanofibres containing collagen than PCL alone. Taken together, multiscale scaffolds with diverse functionality and tunable distribution of biomolecules across the nanofibrous scaffold can be fabricated using electrospun nanofibres.

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Fiber diameter and texture of electrospun PEOT/PBT scaffolds influence human mesenchymal stem cell proliferation and morphology, and the release of incorporated compounds

Cited by 237 publications

References 50 publications

The Creation of Electrospun Nanofibers from Platelet Rich Plasma

The Creation of Electrospun Nanofibers from Platelet Rich Plasma

Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

Multifunctional nanofibrous scaffold for tissue engineering

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