Fiber-optic biosensor to assess circulating phagocyte activity by chemiluminescence

Magrisso, Moni J.; Etzion, Ohad; Pilch, Greg; Novodvoretz, Alex; Perez-Avraham, Galit; Schlaeffer, Francisc; Marks, Robert S.

doi:10.1016/j.bios.2005.05.006

Cited by 26 publications

(25 citation statements)

References 26 publications

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“…OZ was prepared fresh just before each experiment. KRP was composed of 119 mmol/L NaCl, 4.75 mmol/L KC1, 0.420 mmol/L CaCl2, 1.19 mmol/L, MgSO4·7H2O, 16.6 mmol/L sodium phosphate buffer, pH 7.4 and 5.56 mmol/L glucose (1).…”

Section: Preparation Of Stimulantsmentioning

confidence: 99%

“…A novel luminometer for a fiber array-based biosensor was developed in our laboratory to monitor circulating phagocyte activity in whole blood (1). In order to better understand the mechanisms of reactive oxygen species (ROS) production by neutrophils, establishment of models based on a phagocyte cell line was needed.…”

Section: Introductionmentioning

confidence: 99%

“…Luminol-dependent chemiluminescence (LCL) is an important method for real-time studies of ROS production by activated neutrophils (1,14,15) and was used to compare between the two cell lines. LCL allows measurement of intracellular as well as extra-cellular reactions in response to different stimulants (14,16,17) and was shown to be dependent on the MPO-H 2 O 2 reaction (18,19).…”

Section: Introductionmentioning

confidence: 99%

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Luminol‐dependent chemiluminescence of human phagocyte cell lines: comparison between DMSO differentiated PLB 985 and HL 60 cells

Ashkenazi

Marks

2009

Luminescence

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The human promyelocytic leukemia HL 60 and PLB 985 cell lines can differentiate into terminally mature neutrophil-like cells via dimethyl sulfoxide (DMSO) induction. In this study the luminol-dependent chemiluminescence (LCL) of both neutrophil-like cells was analayzed and compared in response to phorbol myristate acetate (PMA) and opsonized zymosan (OZ) stimulants. It was shown that, like human blood neutrophils, both neutrophil-like cells expressed high levels of CD11b, but unlike human blood neutrophils these cells almost lack LCL-detectable intracellular oxidase activity. By studying the pattern of activation to OZ and PMA and priming with GM-CSF, we concluded that there is no difference between the percentage of differentiation and function of DMSO-induced HL 60 and PLB 985. However, the LCL capacity (area under the curve) of DMSO induced PLB 985 cells was higher than that of HL 60 cells in response to both PMA and OZ, which implies a higher capacity to generate reactive oxygen species in PLB 985 cells.

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Section: Preparation Of Stimulantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Luminol‐dependent chemiluminescence of human phagocyte cell lines: comparison between DMSO differentiated PLB 985 and HL 60 cells

Ashkenazi

Marks

2009

Luminescence

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“…In last two decades, significant progress has been made in the field of evanescence wave-induced fluorescence/chemiluminescencebased fiber-optic biosensors. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] Despite these advantages, the fiber-optic biosensors have been used, most of the time, only in detection of various kinds of biological analytes. 5,6,8,[11][12][13][14][15][16] Work on quantitative estimation of bioanalytes using fiber-optic sensors is very limited.…”

Section: Introductionmentioning

confidence: 99%

“…20 Most of the existing fluorescence/chemiluminescence systems use fluorimeter setup for detection of the fluorescence/ luminescence signal. 2,8,9,[11][12][13][14][15] We have used a charged-coupled device (CCD) based spectrometer to record the full spectral profile of the fluorescence signal. 6,10,16 The Least squares fitting method was used to extract a signal from recorded fluorescence spectral profile with the significantly enhanced signalto-background ratio.…”

Section: Introductionmentioning

confidence: 99%

Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

et al. 2010

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Abstract.A combination tapered fiber-optic biosensor (CTFOB) dip probe for rapid and cost-effective quantification of proteins in serum samples has been developed. This device relies on diode laser excitation and a charged-coupled device spectrometer and functions on a technique of sandwich immunoassay. As a proof of principle, this technique was applied in a quantitative estimation of interleukin IL-6. The probes detected IL-6 at picomolar levels in serum samples obtained from a patient with lupus, an autoimmune disease, and a patient with lymphoma. The estimated concentration of IL-6 in the lupus sample was 5.9 ± 0.6 pM, and in the lymphoma sample, it was below the detection limit. These concentrations were verified by a procedure involving bead-based xMAP technology. A similar trend in the concentrations was observed. The specificity of the CTFOB dip probes was assessed by analysis with receiver operating characteristics. This analysis suggests that the dip probes can detect 5-pM or higher concentration of IL-6 in these samples with specificities of 100%. The results provide information for guiding further studies in the utilization of these probes to quantify other analytes in body fluids with high specificity and sensitivity. C 2010 Society of Photo-Optical Instrumentation Engineers.

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Fiber-Optic Based Cell Sensors

Eltzov

Marks

2009

Whole Cell Sensing Systems I

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Different whole cell fiber optic based biosensors have been developed to detect the total effect of a wide range of environmental pollutants, providing results within a very short period. These biosensors are usually built from three major components, the biorecognition element (whole-cells) intimately attached to a transducer (optic fiber) using a variety of techniques (adsorption, covalent binding, polymer trapping, etc). Even with a great progress in the field of biosensors, there is still a serious lack of commercial applications, capable of competing with traditional analytical tools.

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Fiber-optic biosensor to assess circulating phagocyte activity by chemiluminescence

Cited by 26 publications

References 26 publications

Luminol‐dependent chemiluminescence of human phagocyte cell lines: comparison between DMSO differentiated PLB 985 and HL 60 cells

Luminol‐dependent chemiluminescence of human phagocyte cell lines: comparison between DMSO differentiated PLB 985 and HL 60 cells

Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

Fiber-Optic Based Cell Sensors

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