Fiber Optic Microfluorimetry for Acute and Chronic In-Vivo Animal Studies

Goldstein, Seth; Bonner, Robert F.; Dedrick, Robert L.; Grantham, Flora H.; Gullino, Pietro M.; Gibson, Carter C.; McGuire, Douglas

doi:10.1115/1.3149584

Cited by 19 publications

(5 citation statements)

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“…A preferential distribution of ALA‐derived porphyrins in tumor cells provides the possibility of in situ detection of the porphyrin fluorescence in the superficial transformed epithelial cells of the skin and internal hollow organs by means of fiberoptic point monitoring systems or of fluorescence imaging systems 220, 221. Such a procedure can be performed noninvasively in vivo with immediate results and can also be used for pharmacokinetic studies 83, 220‐223. Topical and local internal administration of ALA may be used for such diagnostic purposes because intravesical, intrauterine, and inhalant administrations do not produce generalized cutaneous photosensitivity.…”

Section: Major Current Challengesmentioning

confidence: 99%

5-Aminolevulinic acid-based photodynamic therapy

Peng

Warloe

Berg

et al. 1997

Cancer

926

534

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BACKGROUND Photodynamic therapy (PDT) for cancer patients has developed into an important new clinical treatment modality in the past 25 years. PDT involves administration of a tumor‐localizing photosensitizer or photosensitizer prodrug (5‐aminolevulinic acid [ALA], a precursor in the heme biosynthetic pathway) and the subsequent activation of the photosensitizer by light. Although several photosensitizers other than ALA‐derived protoporphyrin IX (PpIX) have been used in clinical PDT, ALA‐based PDT has been the most active area of clinical PDT research during the past 5 years. Studies have shown that a higher accumulation of ALA‐derived PpIX in rapidly proliferating cells may provide a biologic rationale for clinical use of ALA‐based PDT and diagnosis. However, no review updating the clinical data has appeared so far. METHODS A review of recently published data on clinical ALA‐based PDT and diagnosis was conducted. RESULTS Several individual studies in which patients with primary nonmelanoma cutaneous tumors received topical ALA‐based PDT have reported promising results, including outstanding cosmetic results. However, the modality with present protocols does not, in general, appear to be superior to conventional therapies with respect to initial complete response rates and long term recurrence rates, particularly in the treatment of nodular skin tumors. Topical ALA‐PDT does have the following advantages over conventional treatments: it is noninvasive; it produces excellent cosmetic results; it is well tolerated by patients; it can be used to treat multiple superficial lesions in short treatment sessions; it can be applied to patients who refuse surgery or have pacemakers and bleeding tendency; it can be used to treat lesions in specific locations, such as the oral mucosa or the genital area; it can be used as a palliative treatment; and it can be applied repeatedly without cumulative toxicity. Topical ALA‐PDT also has potential as a treatment for nonneoplastic skin diseases. Systemic administration of ALA does not seem to be severely toxic, but the advantage of using this approach for PDT of superficial lesions of internal hollow organs is still uncertain. The ALA‐derived porphyrin fluorescence technique would be useful in the diagnosis of superficial lesions of internal hollow organs. CONCLUSIONS Promising results of ALA‐based clinical PDT and diagnosis have been obtained. The modality has advantages over conventional treatments. However, some improvements need to be made, such as optimization of parameters of ALA‐based PDT and diagnosis; increased tumor selectivity of ALA‐derived PpIX; better understanding of light distribution in tissue; improvement of light dosimetry procedure; and development of simpler, cheaper, and more efficient light delivery systems. Cancer 1997; 79:2282‐308. © 1997 American Cancer Society.

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Section: Major Current Challengesmentioning

confidence: 99%

5-Aminolevulinic acid-based photodynamic therapy

Peng

Warloe

Berg

et al. 1997

Cancer

926

534

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“…Goldstein et a2. [33] have developed a fiber optic microfluorimetric technique for measurement of blood clearance and tissue uptake response characteristics of fluorescein conjugates in tumors. The uncertainties associated with hemoglobin absorption effects have important consequences, in light of the present findings, as fiber optic techniques are not amenable to plasma layer monitoring.…”

Section: / Platementioning

confidence: 99%

Transport of Macromolecules In Tumor Microcirculation

Jain

1985

Biotechnology Progress

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“…8 The use of fiber optics have shown promise of measuring in situ transport. For example, Goldstein et al 9 have demonstrated that multiple fiber optic probes can be used in the investigation of solute transport of a fluorescence dye in tissues by implantation. Yet, to obtain reasonable spatial resolution they found the need to use an array of individual sensors and then combine the responses of each sensor to generate a transport map.…”

Section: Introductionmentioning

confidence: 99%

Remote semiquantitative two-dimensional flow transport imaging using microendoscopic probes

Houlne¹

1997

Opt. Eng

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A flexible microscope is configured, evaluated, and employed in the noninvasive investigation of fluid transport in a tissuelike model or porous, fibrous matrix. A simple optical configuration that employs microendoscopic probes, an objective lens, and a commercial CCD for detection, produces resolution performance in the range of 25 to 40 line pairs/mm (lp/mm). Remote, time-sequenced imaging is shown to be possible using this moderate resolution flexible microscope. The microendoscopes employed are 1 to 3 m in length and range in outer diameter from 1.5 to 2.5 mm; some contain a working lumen for the introduction of a tool or a fluid at the site of investigation. Target lighting is accomplished by an integral fiber bundle built into the endoscope and supplied from an external xenon discharge lamp. This instrumentation is combined with specialized image processing for quantitative, time-sequenced imaging of marker dye transport on a tissuelike model substrate. Quantitative absorbance imaging is accomplished by calibrating response for marker dye standards on the test substrate and correlating camera pixel grayscale values to solute concentration. The quantitative mapping (concentration versus time and position) of fluid transport is used to evaluate convective versus diffusive flow. © 1997 Society of Photo-Optical Instrumentation Engineers. [S0091-3286(97)02907-3]

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Fiber Optic Microfluorimetry for Acute and Chronic In-Vivo Animal Studies

Cited by 19 publications

References 0 publications

5-Aminolevulinic acid-based photodynamic therapy

5-Aminolevulinic acid-based photodynamic therapy

Transport of Macromolecules In Tumor Microcirculation

Remote semiquantitative two-dimensional flow transport imaging using microendoscopic probes

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