Fiber-Optic Raman Probes for Biomedical and Pharmaceutical Applications

Sato, Hidetoshi; Shinzawa, Hideyuki; Komachi, Yuichi

doi:10.1007/978-3-642-02649-2_2

Cited by 8 publications

(11 citation statements)

References 52 publications

(60 reference statements)

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“…Some are designed to contact a specimen, whereas others are noncontact and rely on lenses to deliver laser light and collect Raman scatter. The topic has been reviewed by Sato et al 22 The simplest probe design uses a single optical fiber to transmit laser light and collect backscattered Raman signal. A dichroic filter or other optic is used to separate the exciting laser beam and signal.…”

Section: Fiber Optic Probesmentioning

confidence: 99%

Contributions of Raman spectroscopy to the understanding of bone strength

Mandair¹,

Morris²

2015

BoneKEy Reports

291

314

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Raman spectroscopy is increasingly commonly used to understand how changes in bone composition and structure influence tissue-level bone mechanical properties. The spectroscopic technique provides information on bone mineral and matrix collagen components and on the effects of various matrix proteins on bone material properties as well. The Raman spectrum of bone not only contains information on bone mineral crystallinity that is related to bone hardness but also provides information on the orientation of mineral crystallites with respect to the collagen fibril axis. Indirect information on collagen cross-links is also available and will be discussed. After a short introduction to bone Raman spectroscopic parameters and collection methodologies, advances in in vivo Raman spectroscopic measurements for animal and human subject studies will be reviewed. A discussion on the effects of aging, osteogenesis imperfecta, osteoporosis and therapeutic agents on bone composition and mechanical properties will be highlighted, including genetic mouse models in which structure-function and exercise effects are explored. Similarly, extracellular matrix proteins, proteases and transcriptional proteins implicated in the regulation of bone material properties will be reviewed.

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Section: Fiber Optic Probesmentioning

confidence: 99%

Contributions of Raman spectroscopy to the understanding of bone strength

Mandair¹,

Morris²

2015

BoneKEy Reports

291

314

View full text Add to dashboard Cite

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“…1–8 The development of spatially offset Raman spectroscopy 9–12 and transmission Raman spectroscopy 13 has allowed depth resolved probing, 14 and rapid spectroscopy 15 . However, many systems often have variable albedo.…”

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confidence: 99%

“…While the silica bands of commonly employed fibers might serve this function, in practice they are sufficiently broad that their effect is to create broad backgrounds that, together with specimen fluorescence become difficult to remove accurately. 1 An alternative is to generate a narrow reference Raman signal from the fiber itself or from a material attached to it. We report here the use of fluorinated ethylene propylene copolymer (FEP) to generate a reference Raman signal proportional to delivered laser power.…”

mentioning

confidence: 99%

Polymer-capped fiber-optic Raman probe for non-invasive Raman spectroscopy

Okagbare

Morris

2012

Analyst

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Advances in fiber optic probe design are moving Raman spectroscopy into the clinic, although there remain important practical problems. While much effort has been devoted to minimizing Raman and fluorescence background from fibers, less attention has been given to the need to generate reference Raman signals that can correct for variations in tissue albedo, which is important in quantifying changes in tissue composition. To address this shortcoming, we have developed a fiber optic probe that incorporates a fluorinated ethylene-propylene copolymer (FEP) cap at the end of each excitation fiber. Transmission of laser light through the transparent cap generates a 732 cm−1 Raman band whose intensity scales linearly with the laser power delivered to the tissue of interest. In our first design, the FEP cap functions as a waveguide with only a small insertion loss (~5%). Laser transmission through 1 mm of the polymer is sufficient to generate a usable reference Raman signal. We show the application of the probe to quantitative non-invasive Raman spectroscopy of animal tissues using rat leg phantoms as models. Ex-vivo Raman spectroscopy of excised rat tibia supports the use of the probe for spectroscopy of various tissues. These results provide proof of principle that the Raman probe can be used in multiple spectroscopic applications.

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“…Transmission Raman, involving collection of Raman photons through a sample, was later developed for noninvasive forensic and pharmaceutical analyses [3,4]. Since those first reports in SORS and transmission Raman, we have seen advances in fiber optic probe design, Raman-scattered photon transport theory, data preprocessing and tissue phantom models [5][6][7][8][9][10]. There is potential of transcutaneous Raman spectroscopy, either in a SORS or transmission approach, for clinical applications in bone disease and breast cancer [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Next-generation Raman tomography instrument for non-invasive in vivo bone imaging

Demers¹,

Esmonde-White²,

Esmonde-White³

et al. 2015

Biomed. Opt. Express

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Combining diffuse optical tomography methods with Raman spectroscopy of tissue provides the ability for in vivo measurements of chemical and molecular characteristics, which have the potential for being useful in diagnostic imaging. In this study a system for Raman tomography was developed and tested. A third generation microCT coupled system was developed to combine 10 detection fibers and 5 excitation fibers with laser line filtering and a Cytop reference signal. Phantom measurements of hydroxyapatite concentrations from 50 to 300 mg/ml had a linear response. Fiber placement and experiment design was optimized using cadaver animals with live animal measurements acquired to validate the systems capabilities. Promising results from the initial animal experiments presented here, pave the way for a study of longitudinal measurements during fracture healing and the scaling of the Raman tomography system towards human measurements. ©2015 Optical Society of America

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Fiber-Optic Raman Probes for Biomedical and Pharmaceutical Applications

Cited by 8 publications

References 52 publications

Contributions of Raman spectroscopy to the understanding of bone strength

Contributions of Raman spectroscopy to the understanding of bone strength

Polymer-capped fiber-optic Raman probe for non-invasive Raman spectroscopy

Next-generation Raman tomography instrument for non-invasive in vivo bone imaging

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