Fibrillin expression and localization in various types of carcinomas of the thyroid gland

Tseleni‐Balafouta, Sofia; Gakiopoulou, Hariklia; Fanourakis, Galinos; Voutsinas, Gerassimos E.; Litsiou, Helen; Sozopoulos, Elias; Balafoutas, Dimitrios; Patsouris, Efstratios

doi:10.1038/modpathol.3800578

Cited by 11 publications

(4 citation statements)

References 22 publications

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“…All these genes were chosen based on their functional involvement in breast cancer. Up regulated genes included s elenoprotein plasma protein (SEPP1) and decorin (DCN) which have an antimetastatic role in breast cancer (22-24) fibrillin1 (FBN1), aldehyde oxidase 1 (AOX1) and prostagladin E receptor 2 (PTERG2) which are known to promote tumor growth (25-27) and, surprisingly, angiopotein1 (ANGPT1 ) which when over expressed has antitumor properties (28). …”

Section: Resultsmentioning

confidence: 99%

Cytogenetic and cDNA Microarray Expression Analysis of MCF10 Human Breast Cancer Progression Cell Lines

et al. 2009

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We used a combination of spectral karyotyping, array comparative genomic hybridization, and cDNA microarrays to gain insights into the structural and functional changes of the genome in the MCF10 human breast cancer progression model cell lines. Spectral karyotyping data showed several chromosomal aberrations and array comparative genomic hybridization analysis identified numerous genomic gains and losses that might be involved in the progression toward cancer. Analysis of the expression levels of genes located within these genomic regions revealed a lack of correlation between chromosomal gains and losses and corresponding up-regulation or downregulation for the majority of the f1,000 genes analyzed in this study. We conclude that other mechanisms of gene regulation that are not directly related to chromosomal gains and losses play a major role in breast cancer progression. [Cancer Res 2009;69(14):5946-53]

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Section: Resultsmentioning

confidence: 99%

Cytogenetic and cDNA Microarray Expression Analysis of MCF10 Human Breast Cancer Progression Cell Lines

et al. 2009

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“…Although the mutation of FBN1 is a main pathogenesis of Marfan syndrome and Weill-Marchesani syndrome [41, 42], the overexpression of FBN1 is also detected in cancer tissues [43]. Several studies have shown that FBN1 is associated with TGF-beta signaling [44, 45], while the TGF-beta signaling is reportedly to induce EMT that may in turn regulate the expressions of E-cadherin, beta-catenin, and MMPs [46–49].…”

Section: Discussionmentioning

confidence: 99%

Fibrillin-1, induced by Aurora-A but inhibited by BRCA2, promotes ovarian cancer metastasis

et al. 2015

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While Aurora-A (Aur A) provokes, BRCA2 restrains primary tumorigenesis, the roles of Aur A and BRCA2 in cancer metastasis remains unclear. Here, we show that the metastatic promoting markers SLUG, FBN1, and MMP2, 9, 13 are either stimulated or suppressed by Aur A or BRCA2, but the metastatic suppressors E-cadherin, β-catenin, and p53 are either inhibited or promoted by Aur A or BRCA2, leading to enhanced or reduced cell migration and invasion. Further study suggests that FBN1 inhibits E-cadherin and β-catenin, but stimulates MMP2, 9, 13. Depletion of SLUG abrogates FBN1 and MMP9, but increases E-cadherin, while p53 decreases both SLUG and FBN1. Animal assays demonstrate that FBN1 promotes both ovarian tumorigenesis and metastasis. Clinically, overexpression of BRCA2 or Aur A in ovarian cancer tissues predicts good or poor overall and disease free survivals. High expression of SLUG or FBN1 indicates poor overall survivals, whereas high expression of FBN1 but not of SLUG predicts poor disease free survival. No significant associations between p53 expression and patient survivals were found. Overall, FBN1, acts at the downstream of Aur A and BRCA2, promotes ovarian cancer metastasis through the p53 and SLUG-associated signaling, which may be useful for ovarian cancer diagnosis and treatment.

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“…FBN2 (fibrillin 2), which is homologous to FBN1, is highly expressed in embryonic tissues [Samuel et al, 2003]. Recently, FBN1 has been shown to play a key role in lung development [Chen et al, 2005] and has been implicated in cell attachment, differentiation, and migration [Tseleni-Balafouta et al, 2006]. The well-known hSSC marker c-kit is essential for the maintenance of PGCs in both sexes.…”

Section: Discussionmentioning

confidence: 98%

Expression profile of genes identified in human spermatogonial stem cell‐like cells using suppression subtractive hybridization

Yoo

Lim

et al. 2010

J of Cellular Biochemistry

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Spermatogenesis is the process by which testicular spermatogonial stem cells (SSCs) self-renew and differentiate into mature sperm in the testis. Maintaining healthy spermatogenesis requires proper proliferation of SSCs. In this study, we sought to identify factors that regulate the proliferation of SSCs. Human SSC (hSSC)-like cells were isolated from azoospermic patients by a modified culture method and propagated in vitro. After four to five passages, the SSC-like cells spontaneously ceased proliferating in vitro, so we collected proliferating (P)-hSSC-like cells at passage two and senescent (S)-hSSC-like cells at passage five. Suppression subtractive hybridization (SSH) was used to identify genes that were differentially expressed between the P-hSSC-like and S-hSSC-like cells. We selected positive clones up-regulated in P-hSSC-like cells using SSH and functionally characterized them by reference to public databases using NCBI BLAST tools. Expression levels of genes corresponding to subtracted clones were analyzed using RT-PCR. Finally, we confirmed the differential expression of 128 genes in positive clones of P-hSSC-like cells compared with S-hSSC-like cells and selected 23 known and 39 unknown clones for further study. Known genes were associated with diverse functions; 22% were related to metabolism. Fifteen of the known genes and two of the unknown genes were down-regulated after senescence of hSSC-like cells. A comparison with previous reports further suggests that known genes selected, SPP1, may be related to germ cell biogenesis and cellular proliferation. Our findings identify several potential novel candidate biomarkers of proliferating- and senescencet-hSSCs, and they provide potentially important insights into the function and characteristics of human SSCs.

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Fibrillin expression and localization in various types of carcinomas of the thyroid gland

Cited by 11 publications

References 22 publications

Cytogenetic and cDNA Microarray Expression Analysis of MCF10 Human Breast Cancer Progression Cell Lines

Cytogenetic and cDNA Microarray Expression Analysis of MCF10 Human Breast Cancer Progression Cell Lines

Fibrillin-1, induced by Aurora-A but inhibited by BRCA2, promotes ovarian cancer metastasis

Expression profile of genes identified in human spermatogonial stem cell‐like cells using suppression subtractive hybridization

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