Fibrin glue mixed with platelet-rich fibrin as a scaffold seeded with dental bud cells for tooth regeneration

Yang, Kai-Chiang; Wang, Chun‐Hao; Chang, Hao Hueng; Chan, Wing P.; Hwa, Chau; Kuo, Ting‐Shen

doi:10.1002/term.483

Cited by 56 publications

(32 citation statements)

References 39 publications

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“…PRF can be thought of as a cytokine bank that contains roughly all of the cytokines in the platelets 8) . Importantly, the growth factors obtained from PRF may not only have their own specific action on tissues, but may also interact with other growth factors.…”

Section: Discussionmentioning

confidence: 99%

New Biodegradable Implant Material Containing Hydrogel with Growth Factors of Lyophilized PRF in Combination with an nHA/PLGA Scaffold

Zheng

Wang

Qin

et al. 2015

J. Hard Tissue Biology.

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The temperature-sensitive triblock copolymer poly-(D, L-lactic acid-co-glycolic acid) (PLGA)-polyethylene glycol (PEG)-PLGA (PLGA-PEG-PLGA) is an FDA-approved material that has the ability to provide a sustained release of drugs and/or proteins. Platelet-rich fibrin(PRF)is second generation platelet concentration that contains growth factors such as transforming growth factor-1 (TGF-1), platelet derived growth factor-AB (PDGF-AB), and insulin-like growth factor-I (IGF-I). These growth factors affect the migration and proliferation of diverse cell types, including endothelial cells, smooth muscle cells, and osteoblast-like cells. This study sought to combine the hydrogel into scaffolds in order to serve as a sustained release system for PRF-derived growth factors. Poly (lactic-co-glycolic) acid (PLGA) and nano-hydroxyapatite (nHA) were used to prepare the hydrogel-containing scaffolds with the PRF-derived growth factors. We then investigated the effects of the hydrogel on modulating the activity of osteoblasts in vitro. We indicated that the hydrogel (Gel) was well-distributed in the inner surface of scaffolds, which themselves exhibited relatively interconnected pores with uniform sizes. The addition of the hydrogel didn't affect their inherently high porosity. In vitro release tests indicated that the system containing nHA/PLGA/Gel/PRF provided for a slow and sustained release of PRF-derived growth factors. The results from our in vitro studies indicated that the MG63 cells cultured with both scaffold media extracts did not appear to have cytotoxic responses, and the nHA/PLGA/Gel/PRF system could improve the adhesion and proliferation of MG63 cells when compared to controls (p < 0.05). This in vitro evaluation suggests that the hydrogel-scaffold system is suitable as a model for bone tissue engineering, and that it allows for the sustained release of growth factors to improve bone reconstruction.

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Section: Discussionmentioning

confidence: 99%

New Biodegradable Implant Material Containing Hydrogel with Growth Factors of Lyophilized PRF in Combination with an nHA/PLGA Scaffold

Zheng

Wang

Qin

et al. 2015

J. Hard Tissue Biology.

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“…DFSCs are able to form cementum and bone tissues in vitro and in vivo (Kemoun et al, 2007;Yokoi et al, 2006). Human dental epithelial stem cells (hDESCs) can be isolated from the third molar that develops late after birth (Honda et al, 2007;Honda et al, 2005).…”

Section: Cells In Vitro and In Vivomentioning

confidence: 99%

Stem cell-based approaches in dentistry

Mitsiadis

Orsini

Jiménez-Rojo

2015

eCM

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Repair of dental pulp and periodontal lesions remains a major clinical challenge. Classical dental treatments require the use of specialised tissue-adapted materials with still questionable efficacy and durability. Stem cell-based therapeutic approaches could offer an attractive alternative in dentistry since they can promise physiologically improved structural and functional outcomes. These therapies necessitate a sufficient number of specific stem cell populations for implantation. Dental mesenchymal stem cells can be easily isolated and are amenable to in vitro expansion while retaining their stemness. In vivo studies realised in small and large animals have evidenced the potential of dental mesenchymal stem cells to promote pulp and periodontal regeneration, but have also underlined new important challenges. The homogeneity of stem cell populations and their quality control, the delivery method, the quality of the regenerated dental tissues and their integration to the host tissue are some of the key challenges. The use of bioactive scaffolds that can elicit effective tissue repair response, through activation and mobilisation of endogenous stem cell populations, constitutes another emerging therapeutic strategy. Finally, the use of stem cells and induced pluripotent cells for the regeneration of entire teeth represents a novel promising alternative to dental implant treatment after tooth loss. In this mini-review, we present the currently applied techniques in restorative dentistry and the various attempts that are made to bridge gaps in knowledge regarding treatment strategies by translating basic stem cell research into the dental practice.

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“…After 36 weeks, these implants developed into a complete tooth or an unerupted tooth crown. [98] For reassembly of porcine tooth bud cells implanted in the extraction socket. After 36 weeks, these implants developed into a complete tooth or an unerupted tooth crown.…”

Section: Naturally Derived Scaffold:-alginate:-mentioning

confidence: 99%

“…After 36 weeks, these implants developed into a complete tooth or an unerupted tooth crown. [98] Injectable Scaffold Delivery:-Rigid tissue engineered scaffold structures provide excellent support for cells used in bone and other body areas where the engineered tissue is required to provide physical support [99]. However, in root canal systems a tissue engineered pulp is not required to provide structural support of the tooth.…”

Section: Naturally Derived Scaffold:-alginate:-mentioning

confidence: 99%