Fibrinogen stability under surfactant interaction

Hassan, Natalia; Barbosa, Leandro R.S.; Itri, Rosângela; Ruso, Juan M.

doi:10.1016/j.jcis.2011.06.010

Cited by 37 publications

(18 citation statements)

References 54 publications

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“…This conclusion is further supported by a comparison of the molecular mass ( M m ) estimates based on SAXS and raw sequence data. With regard to fibrinogen, our SAXS data indicate that it appears in solution as a parallel‐paired dimer, which is consistent with the SAXS results published by others .…”

Section: Resultssupporting

confidence: 92%

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Borrelia outer surface protein C is capable of human fibrinogen binding

et al. 2019

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Outer surface protein C (OspC) is one of the most abundant surface lipoproteins produced during early infection by the Borrelia spirochete, the causative agent of Lyme disease. The high sequence variability of the ospC gene results in the production of several and strongly divergent OspC types. One of the known roles of OspC is the recruitment of blood components, including complement regulators, to facilitate the bloodstream survival of Borrelia at an essential stage of host infection. Here, we identify and describe a new interaction between OspC and human fibrinogen. To test the ability of OspC to bind fibrinogen, we developed a microscale thermophoresis assay using four fluorescently labeled types of OspC. We show that OspC binds fibrinogen tightly, with nanomolar Kd, and that the binding depends on the OspC type. The binding assays combined with SAXS studies allowed us to map the OspC‐binding site on the fibrinogen molecule. Spectrometric measurements of fibrinogen clotting in the presence of OspC indicate that OspC negatively influences the clot formation process. Taken together, our findings are consistent with the hypothesis that OspC interacts with blood protein partners to facilitate Borrelia spreading by the hematogenous route.

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Section: Resultssupporting

confidence: 92%

“…With regard to fibrinogen, our SAXS data indicate that it appears in solution as a parallel-paired Table 3. dimer, which is consistent with the SAXS results published by others [35].…”

supporting

confidence: 93%

Borrelia outer surface protein C is capable of human fibrinogen binding

et al. 2019

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“…[1][2][3] For years, single-molecule studies based on fluorescence experiments have been used to investigate transitions between different DNA conformations. [1][2][3] For years, single-molecule studies based on fluorescence experiments have been used to investigate transitions between different DNA conformations.…”

Section: Introductionmentioning

confidence: 99%

“…Biomacromolecules interactions are ubiquitous in biotechnology and biological applications. [1][2][3] For years, single-molecule studies based on fluorescence experiments have been used to investigate transitions between different DNA conformations. By using a wide variety of compacting agents, double-stranded DNA in its coil (extended) conformation can undergo a transition into its globular (compact) conformation.…”

Section: Introductionmentioning

confidence: 99%

A Versatile Approach towards the Compaction, Decompaction, and Immobilization of DNA at Interfaces by Using Cyclodextrins

González‐Pérez

Ruso

2013

ChemPhysChem

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We investigate the temperature dependence of interactions of β-cyclodextrin (CD)/hexadecyltrimethylammonium bromide (CTAB) self-assemblies with DNA during the decompaction of DNA/CTAB complexes. By combining direct imaging techniques with density and sound-velocity measurements, we can explain the decompaction process and suggest a suitable model. The DNA-decompaction process by using CDs is accompanied by interactions with surfaces, such as glass or mica. The mechanism of β-CD/CTAB self-assembly is elucidated and the immobilization of DNA onto negatively charged surfaces is explained. Differences between the fractal dimensions of DNA that is adsorbed onto the surfaces are related to strong and weak binding, which permit the partial relaxation of DNA on the surfaces. The β-CD/CTAB self-assembled monolayers are demonstrated to be a facile and efficient route for surface functionalization, which allows for the immobilization of biomacromolecules in close proximity without any intermediate binding or deprotection steps. Moreover, this route is expected to show several advantages that might contribute to improving the performance of future biosensors as gentle immobilization-limiting alteration of the protein structure, oriented immobilization, thereby allowing homogeneous accessibility, reversible immobilization, thereby allowing reutilizations, and high compatibility with various types of biomacromolecules.

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“…As curvas p(r) experimentais foram obtidas usando o programa GNOM. Os ajustes mostrados junto com a p(r) experimental foram realizados com a combinação linear das curvas de p(r) teóricas obtidas para a estrutura cristalográfica da HbLt, mostradas na Figura Vários estudos, baseados na técnica de SAXS, são reportados na literatura para os processos de dissociação, desnaturação e agregação para diferentes proteínas, induzidos por variação de pH, altas concentrações de sal e presença de cloreto de guanidina [44,45,49,[61][62][63][64][65][66].…”

Section: Fonte-unclassified

Estudo da estabilidade térmica da hemoglobina extracelular gigante de Glossoscolex paulistus (HbGp): efeitos do estado de oxidação do ferro do grupo heme, pH e presença de surfactante

Carvalho¹

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AgradecimentosAgradeço! Aos meus pais Maria Gorete Pires Carvalho e José de Ribamar Gomes Carvalho e a minha esposa Sumária Sousa e Silva pela dedicação, carinho, motivação nas horas mais difíceis, sem eles não teria forças para seguir essa jornada; Ao Professor Marcel Tabak por sua orientação e pelos laços de amizade e respeito que se formaram nesta convivência e também pela oportunidade de poder desenvolver esse trabalho;A Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) pela bolsa de doutorado;Aos meus irmãos Genilson, Genilda e Gesilda pelo apoio, carinho e afeto que temos e pelas palavras amigas;Às minhas tias Eva (segunda mãe), Maria da Guia e Raimunda Teresa e os primos (as) Rita de Kássia, Karla Karolina, Raiza, Rairone, Railson, Amanda e Constância pelo apoio e carinho que sempre me motiva; Aos meus amigos de laboratório Adriano, Fernanda, Ana Eliza, Diógenes e Ezer Biazin, pelo apoio moral e pela contribuição direta e indireta na minha formação e neste trabalho. Obrigado em especial a vocês Adriano e Ezer Biazin; Aos meus amigos Orlando, Ana Célia, Adriane, Washington, Alexandre, Tairo, Adriana, Elenice, Jairo e Edvan pelas discussões bem humoradas e pela amizade; Aos professores Dr.(a) (s) Leandro R. S. Barbosa (IF-USP), Rosângela Itri (IF-USP), Patríca S. Santiago (UNESP-Registro), Carlos Ernesto Garrido Salmon (USP-Riberão Preto) e Tatiana Batista (UEL-PR) pelas discussões e colaborações durante meu curso de doutorado, que foi muito importante para minha formação; Ao Ezér Biazin, técnico do laboratório, por toda ajuda na parte instrumental e que sem nenhuma dúvida foi muito importante para o bom desenvolvimento deste trabalho e pela amizade que se construiu nestes cinco anos; A Andressa Patrícia Alves Pinto, técnica do laboratório de biofísica do IFSC por toda ajuda nos experimentos de CD que foi muito importante para o bom desenvolvimento deste trabalho; Ao Laboratório Nacional de Luz Síncrotron (LNLS), em especial ao Dr. Mateus Cardoso, coordenador da linha SAXS2, pelo auxílio na realização das medidas de SAXS;Ao pessoal da pós-graduação, Sílvia, Andréia e Gustavo, por toda a atenção, carinho e pelos bons serviços prestados;A dona Suely e seu José de Ribamar pelos conselhos e apoio nos momentos difíceis.

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Fibrinogen stability under surfactant interaction

Cited by 37 publications

References 54 publications

Borrelia outer surface protein C is capable of human fibrinogen binding

Borrelia outer surface protein C is capable of human fibrinogen binding

A Versatile Approach towards the Compaction, Decompaction, and Immobilization of DNA at Interfaces by Using Cyclodextrins

Estudo da estabilidade térmica da hemoglobina extracelular gigante de Glossoscolex paulistus (HbGp): efeitos do estado de oxidação do ferro do grupo heme, pH e presença de surfactante

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