Fibroblast cell interactions with human melanoma cells affect tumor cell growth as a function of tumor progression.

Cornil, Isabelle; Theodorescu, Dan; Man, Shan; Herlyn, Meenhard; Jambrosic, J.; Kerbel, Robert S.

doi:10.1073/pnas.88.14.6028

Cited by 183 publications

(135 citation statements)

References 25 publications

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“…The cell lines of the WM series were kindly provided by Dr Meenhard Herlyn (Wistar Institute, Philadelphia, PA, USA) and have been described in detail elsewhere (MacDougall et al, 1993;Cornil et al, 1991). The MeWo cell line was derived from a lymph node metastasis and its properties have been described elsewhere (Ishikawa et al, 1988a,b).…”

Section: Cell Culturesmentioning

confidence: 99%

Interleukin-6 dependent induction of the cyclin dependent kinase inhibitor p21WAF1/CIP1 is lost during progression of human malignant melanoma

et al. 1999

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Section: Cell Culturesmentioning

confidence: 99%

Interleukin-6 dependent induction of the cyclin dependent kinase inhibitor p21WAF1/CIP1 is lost during progression of human malignant melanoma

et al. 1999

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“…It has been reported that fibroblasts can promote progression of epithelial tumorigenesis (Champs et al, 1990;Chung et al, 1989;Cornil et al, 1991;Gleave et al, 1992;Skobe and Fusenig, 1998). Soluble growth factor(s) derived from fibroblasts should be one of the factors most responsible for the accelerating malignancy (Skobe and Fusenig, 1998), because those fibroblasts might secrete various growth factors such as transforming growth factor ␤ 1 (TGF-␤ 1 ) (Lieubeau et al, 1994;Taipale et al, 1994), interleukin-6 (Lu et al, 1992), platelet-derived growth factor (Ponten et al, 1994), insulin-like growth factors I and II (Ellis et al, 1994;Yee et al, 1991), and hepatocyte growth factor/ epithelial scatter factor (Nakamura et al, 1997).…”

Section: Inflammation Converts Adenoma Cells To Adenocarcinomamentioning

confidence: 99%

Conversion of Human Colonic Adenoma Cells to Adenocarcinoma Cells Through Inflammation in Nude Mice

Okada

Kawaguchi

Habelhah

et al. 2000

Laboratory Investigation

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SUMMARY:The roles of inflammation in the malignant progression of tumors during multistep carcinogenesis have been much discussed but remain to be elucidated. To determine the direct contribution of inflammation to colon carcinogenesis, we established a new model of progression of human colonic adenoma cells using a nude mouse; the progression is accelerated by coimplantation of a plastic plate. The FPCK-1-1 cell line, derived from a colonic polyp in a patient with familial adenomatous polyposis, is nontumorigenic when injected subcutaneously into nude mice in a cell suspension of up to 5 ϫ 10 6 cells per mouse. However implantation of 1 ϫ 10 5 FPCK-1-1 cells attached to a plastic plate induced first acute and then chronic inflammation, and formed progressively growing tumors that were histologically determined as moderately differentiated adenocarcinoma in 65% of mice. Moreover cell lines established from the growing tumors were found to be tumorigenic when injected into mice even without a plastic plate. The tumor arising from the adenoma cells implanted attached to a plastic plate was surrounded by highly proliferating fibrous stroma. This fibrous tissue was considered essential for malignant progression, rather than for attachment to the plastic plate substrate, because the tumors were formed after injection of FPCK-1-1 cells into the fibrous tissue from which the plastic plate had been removed before the cell injection. The conditioned medium (CM) obtained from the fibroblasts derived from a plastic plate-associated stromal tissue was found to contain factors that stimulated growth of FPCK-1-1 cells, but not of the derivative progressor cell lines. The factor was stable to heating and neuraminidase treatment, but labile to trypsin treatment. The main growth-potentiating activity was contained in the fraction larger than 100 kDa. In contrast, the activity to promote FPCK-1-1 cell growth was not present in the CM of subcutaneous fibroblasts from untreated nude mice or the fibroblast cell lines C3H10T 1/2 and NIH3T3. These results demonstrated that inflammation-associated stroma promoted the conversion of colonic adenoma cells to adenocarcinoma cells. (Lab Invest 2000, 80:1617-1628.

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“…Established cell lines from primary melanomas (WM 115, WM 98-1, WM 1341) were kindly supplied by M Herlyn (The Wistar Institute, Philadelphia, PA, USA) (Balaban et al, 1984;Herlyn et al, 1985;Cornil et al, 1991). Cells were grown in Ômelanoma mediumÕ near confluency for the experiments described.…”

Section: Culture Of Melanoma Cell Lines From Primary Melanomasmentioning

confidence: 99%

The chemokine RANTES is secreted by human melanoma cells and is associated with enhanced tumour formation in nude mice

et al. 1999

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Modulation of tumour cell growth by tumour-infiltrating leucocytes is of high importance for the biological behaviour of malignant neoplasms. In melanoma, tumour-associated macrophages (TAM) and tumour-infiltrating lymphocytes (TIL) are of particular interest as inhibitors or enhancers of cell growth. Recruitment of leucocytes from the peripheral blood into the tumour site is mediated predominantly by chemotaxins, particularly by the group of chemokines. The aim of this study was to identify peptides released by human melanoma cells with monocyte chemotactic properties. To assure the presence of biologically active mediators, biochemical purification and biological characterization of peptides was based on a detection system dependent on bioactive, monocyte chemotactic activity in vitro. Cell culture supernatants of melanoma cells were fractioned by heparin–sepharose followed by preparative reversed-phase HPLC steps to enrich monocyte chemotactic activity in one single band on a sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel. These purified fractions were shown to react with RANTES-specific antibodies in an enzyme-linked immunosorbent assay (ELISA) as well as in Western blot analysis. Amino acid sequencing of the N-terminal protein fragment confirmed 100% homology to the RANTES protein. Further analysis showed that four out of eight melanoma cell lines constitutively expressed and secreted the β-chemokine RANTES as detected by ELISA. The amount of RANTES protein secreted (up to 50 ng ml −1 ) was about 5–50 times higher than interleukin 8 (IL-8), determined in the same supernatant samples. Tumour necrosis factor α (TNF-α), not, however, IL-2, interferon-γ (IFN-γ), or α-melanocyte-stimulating hormone (α-MSH) was able to up-regulate RANTES and interleukin 8 secretion. Furthermore, higher levels of RANTES secretion in vitro were associated with increased tumour formation upon S . C . injection of six human melanoma cell lines in nude mice. Our data provide evidence that a subset of melanoma cells express mRNA and secrete RANTES protein which may be partly responsible for the recruitment of monocytes, T-cells and dendritic cells into the tumours. However, transplantation experiments in nude mice suggest that effects of RANTES may also benefit tumour progression. Further studies are needed to dissect the underlying mechanisms. © 1999 Cancer Research Campaign

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Fibroblast cell interactions with human melanoma cells affect tumor cell growth as a function of tumor progression.

Cited by 183 publications

References 25 publications

Interleukin-6 dependent induction of the cyclin dependent kinase inhibitor p21WAF1/CIP1 is lost during progression of human malignant melanoma

Interleukin-6 dependent induction of the cyclin dependent kinase inhibitor p21WAF1/CIP1 is lost during progression of human malignant melanoma

Conversion of Human Colonic Adenoma Cells to Adenocarcinoma Cells Through Inflammation in Nude Mice

The chemokine RANTES is secreted by human melanoma cells and is associated with enhanced tumour formation in nude mice

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