Fibroblast growth factor 10 enhances bovine oocyte maturation and developmental competence in vitro

Abstract

The ability of oocytes to resume meiosis, become fertilized, and generate viable pregnancies is controlled during folliculogenesis by several endocrine and paracrine factors. The aim of this work is to determine whether fibroblast growth factor 10 (FGF10) is an oocyte competent factor. Transcripts for each of the four FGF receptor types (FGFR) were present in cumulus and oocytes after their extraction from the follicles. FGFR1 transcripts predominated in cumulus cells whereas FGFR2 was most abundant in oocytes. Exposing the cumulus-oocyte complexes to FGF10 during in vitro maturation did not affect cleavage rates, but increases (P!0.05) in the percentage of embryos at the 8-16-cell stage on day 3 and at the blastocyst stage on day 7, which were evident in FGF10-supplemented oocytes. The progression of oocytes through meiosis and cumulus expansion was increased (P!0.05) by FGF10. The importance of the endogenous sources of FGFs was examined by adding anti-FGF10 IgG during oocyte maturation. Blocking endogenous FGF10 activity decreased (P!0.05) the percentage of oocytes developing into blastocysts and limited (P!0.05) cumulus expansion. Expression profiles of putative cumulus and oocyte competency markers were examined for their involvement in FGF10-mediated responses. FGF10 influenced the expression of CTSB and SPRY2 in cumulus cells and BMP15 in oocytes. In summary, this work provides new insight into the importance of FGFRs and locally derived FGF10 during oocyte maturation in cattle. Its subsequent impact on in vitro embryo development implicates it as a noteworthy oocyte competent factor.

Bone morphogenetic protein 15 and fibroblast growth factor 10 enhance cumulus expansion, glucose uptake, and expression of genes in the ovulatory cascade during in vitro maturation of bovine cumulus–oocyte complexes

“…Expansion of the cumulus is enhanced by OSFs, including BMP15 and FGF10 (Yoshino et al 2006, Zhang et al 2010, but the mechanisms of action of these growth factors have not been explored. There is also evidence of synergy between BMPs and FGFs, as BMP15 and FGF8 synergize to increase glucose metabolism in mouse cumulus cells (Sugiura et al 2007).…”

“…Cumulus expansion was visually assessed according to a subjective scoring system. Grades 1-3 were attributed to increasing degrees of expansion (1 -poor expansion, characterized by a few morphological changes compared with before maturation; 2 -partial expansion, characterized by fair expansion but notable clusters lacking expansion; 3 -complete or nearly complete expansion; Zhang et al (2010)). …”

“…Another FGF of interest is FGF10, which was localized to the bovine oocyte, and its receptors (FGFR1B and FGFR2B) to cumulus cells (Buratini et al 2007, Cho et al 2008. Interestingly, supplementation of IVM medium with FGF10 enhanced cumulus expansion and embryo development in cattle (Zhang et al 2010), although the mechanism of action is mostly unknown.…”

Effect of Fibroblast Growth Factor 2 (FGF2) and Insulin Transferrin Selenium (ITS) on In Vitro Maturation, Fertilization and Embryo Development in Sheep

“…Porcine FGF7 derived from endometrium stimulated the proliferation of trophoblast cells during the embryonic development (Ka et al 2001). Increased bovine embryo development rates were also observed after supplementation with fibroblast growth factor 10 (FGF10), a theca derived factor (Zhang et al 2010). A FGF of recent interest for a functional role during oocyte maturation is FGF2 (also known as basic FGF).…”

Grb10 characterization in bovine cumulus oocyte complexes from different follicle sizes

“…Local growth factors such as IGF-I, EGF and fibroblast growth factor 10 (FGF10) added into the maturation medium improves the oocyte maturation, resulting in an increase in the in vitro blastocyst rate (WANG et al, 2009;ZHANG et al, 2010). Consequently, tyrosine kinase receptors have important functions on oocyte molecular maturation during follicular development (YAMASHITA & SHIMADA, 2012;YAN et al, 2012).…”