Fibroblast growth factor‐2 regulates the synthesis of hyaluronan by human periodontal ligament cells

Shimabukuro, Yoshio; Ichikawa, Tsuneki; Takayama, Shinichi; Yamada, Satoru; Takedachi, Masahide; Terakura, Mami; Hashikawa, Tomoko; Murakami, Shinya

doi:10.1002/jcp.20256

Cited by 61 publications

(57 citation statements)

References 59 publications

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“…bFGF has multiple effects on cell proliferation, differentiation, and angiogenesis [58]. Animal studies have shown a link between stress, decreased bFGF, and the severity of periodontal disease [19].…”

Section: Blood Flow To the Gingivamentioning

confidence: 99%

Impact of chronic stress on periodontal health

Gunepin¹,

Derache

Trousselard³

et al. 2018

J Oral Med Oral Surg

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--Introduction: Periodontal diseases are caused by pathogenic microorganisms that induce increases in of local and systemic proinflammatory cytokines, resulting in periodontal damage. The onset and evolution of periodontal diseases are influenced by many local and systemic risk factors. Educational objective: In this article, we aim to review the results of the research on the impact of chronic stress on the occurrence, development, and response to periodontal disease treatments and on the pathophysiological mechanisms of periodontal disease. Conclusion: Chronic stress has a negative impact on the occurrence, development, and response to the treatment of periodontal disease via indirect actions on the periodontium. This can result from behavioral changes caused by stress (poor dental hygiene, smoking, etc.) and a direct neuroimmunoendocrinological action related to the consequences (particularly immunological) of the secretion of certain chemicals (e.g., cortisol) induced by the activation of the hypothalamus and the autonomic nervous system in response to stress. These factors necessitate multidisciplinary management (e.g., physician, oral surgeon, and psychologist) of patients to identify subjects with chronic stress and to employ countermeasures to decrease the impact of stress on the periodontium.

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Section: Blood Flow To the Gingivamentioning

confidence: 99%

Impact of chronic stress on periodontal health

Gunepin¹,

Derache

Trousselard³

et al. 2018

J Oral Med Oral Surg

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“…Human PDL (HPDL) cells, dental pulp (HDP) cells, gingival fibroblasts (HGFs), and gingival epithelial cells (HGEs) were cultured according to previously described methods (Murakami et al, 2001(Murakami et al, , 2002Shimabukuro et al, 2005Shimabukuro et al, , 2009). We previously established a mouse PDL cell line, MPDL22, and used this as described in our earlier publication (Yamada et al, 2007).…”

Section: Cell Culture and Induction Of Pdl Cell Cytodifferentiationmentioning

confidence: 99%

Characterization of a Novel Periodontal Ligament-specific Periostin Isoform

et al. 2014

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Periostin is a mesenchymal cell marker predominantly expressed in collagen-rich fibrous connective tissues, including heart valves, tendons, perichondrium, periosteum, and periodontal ligament (PDL). Knockdown of periostin expression in mice results in early-onset periodontitis and failure of cardiac healing after acute myocardial infarction, suggesting that periostin is essential for connective tissue homeostasis and regeneration. However, its role(s) in periodontal tissues has not yet been fully defined. In this study, we describe a novel human isoform of periostin (PDL-POSTN). Isoform-specific analysis by reversetranscription polymerase chain-reaction (RT-PCR) revealed that PDL-POSTN was predominantly expressed in the PDL, with much lower expression in other tissues and organs. A PDL cell line transfected with PDL-POSTN showed enhanced alkaline phosphatase (ALPase) activity and calcified nodule formation, compared with cells transfected with the full-length periostin isoform. A neutralizing antibody against integrin-αv inhibited both ALPase activity and calcified nodule formation in cells transfected with PDL-POSTN. Furthermore, co-immunoprecipitation assays revealed that PDL-POSTN bound to integrin αvβ3 more strongly than the common isoform of periostin, resulting in strong activation of the integrin αvβ3-focal adhesion kinase (FAK) signaling pathway. These results suggest that PDL-POSTN positively regulates cytodifferentiation and mineralization in PDL cells through integrin αvβ3.

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“…Each isoform is the product of a separate gene (11,12). In several cellular systems, alteration in HA biosynthesis is correlated with increases in HAS mRNA pools, although the genes involved differ for different cell types (13)(14)(15)(16)(17)(18)(19). In this study we examined the temporal response of HA and HAS mRNA to activation of keratocytes with TGF␤ and mitogens.…”

Section: Hyaluronan (Ha)mentioning

confidence: 99%

A Rapid Transient Increase in Hyaluronan Synthase-2 mRNA Initiates Secretion of Hyaluronan by Corneal Keratocytes in Response to Transforming Growth Factor β

Guo

Kanter

Funderburgh

et al. 2007

Journal of Biological Chemistry

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Keratocytes of the corneal stroma produce transparent extracellular matrix devoid of hyaluronan (HA); however, in corneal pathologies and wounds, HA is abundant. We previously showed primary keratocytes cultured under serum-free conditions to secrete matrix similar to that of normal stroma, but serum and transforming growth factor ␤ (TGF␤) induced secretion of fibrotic matrix components, including HA. This study found HA secretion by primary bovine keratocytes to increase rapidly in response to TGF␤, reaching a maximum in 12 h and then decreasing to <5% of the maximum by 48 h. Cell-free biosynthesis of HA by cell extracts also exhibited a transient peak at 12 h after TGF␤ treatment. mRNA for hyaluronan synthase enzymes HAS1 and HAS2 increased >10-and >50-fold, respectively, in 4 -6 h, decreasing to near original levels after 24 -48 h. Small interfering RNA against HAS2 inhibited the transient increase of HAS2 mRNA and completely blocked HA induction, but small interfering RNA to HAS1 had no effect on HA secretion. HAS2 mRNA was induced by a variety of mitogens, and TGF␤ acted synergistically to induce HAS2 by as much as 150-fold. In addition to HA synthesis, treatment with TGF␤ induced degradation of fluorescein-HA added to culture medium. These results show HA secretion by keratocytes to be initiated by a rapid transient increase in the HAS2 mRNA pool. The very rapid induction of HA expression in keratocytes suggests a functional role of this molecule in the fibrotic response of keratocytes to wound healing. Hyaluronan (HA)2 is a high molecular weight, nonsulfated glycosaminoglycan abundant in most tissues, where it acts as a hydrating agent and an organizer of extracellular matrix scaffolding via specific interactions with matrix proteins containing hyaluronectin domains (for review see Ref. 1). The corneal stroma, unlike most vertebrate tissues, is virtually devoid of HA. During active corneal wound healing and in corneas with various chronic pathologies, however, hyaluronan becomes abundant in the corneal stroma (2, 3).The corneal stroma maintains transparency to light by virtue of the highly organized structure of its collagenous extracellular matrix. Collagen fibrils of the stroma exhibit highly regular parallel alignment and spacing. This spacing is controlled by collagen-associated small leucine-rich proteoglycans that form glycosaminoglycan cross-links between adjacent fibrils (4). Disruption of the fibril spacing is a major cause of loss of corneal transparency in scarring and stromal pathological conditions (5). In scars, interfibrillar glycosaminoglycan cross-links are altered or eliminated, and spaces without fibrils, known as "lakes," have been identified (5). The almost ubiquitous presence of HA in nontransparent corneas suggests a relationship between the large hydrodynamic volume occupied by HA molecules and the disruption of the stromal ultrastructure. Additionally, the recent recognition of the diverse bioactivity of HA (6) raises the potential that matrix production by stromal keratocytes may...

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Fibroblast growth factor‐2 regulates the synthesis of hyaluronan by human periodontal ligament cells

Cited by 61 publications

References 59 publications

Impact of chronic stress on periodontal health

Impact of chronic stress on periodontal health

Characterization of a Novel Periodontal Ligament-specific Periostin Isoform

A Rapid Transient Increase in Hyaluronan Synthase-2 mRNA Initiates Secretion of Hyaluronan by Corneal Keratocytes in Response to Transforming Growth Factor β

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