2018
DOI: 10.12688/f1000research.14900.1
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Fibroblast growth factor-6 enhances CDK2 and MAKT expression in microvesicles derived from human stem cells extracted from exfoliated deciduous teeth

Abstract: Background: Stem cells from human exfoliated deciduous teeth (SHEDs) are considered one of the most convenient sources of adult stem cells. This study aimed to examine the effect of fibroblast growth factor 6 (FGF-6) on SHEDs and evaluate CDK2 and MATK gene expression in SHED-derived microvesicles (MVs). SHEDs were cultured from deciduous teeth pulp. Methods: SHEDs were divided into two groups: the control group and test groups, with and without FGF-6 supplementation, respectively. After the third passage, SHE… Show more

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Cited by 2 publications
(2 citation statements)
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“…A total of twenty μL of exosomes was loaded onto a formvar/carbon-coated grid, negatively stained with three % aqueous phosphor-tungstic acid for one minute and observed by TEM (HITACHI, H-7650, Japan), which showed their spheroid morphology and confirmed their size [18]. Flow cytometric characterization of exosomes: (FACS analysis) [19]. 1.…”
Section: Transmission Electron Microscope (Tem) Characterization For mentioning
confidence: 97%
“…A total of twenty μL of exosomes was loaded onto a formvar/carbon-coated grid, negatively stained with three % aqueous phosphor-tungstic acid for one minute and observed by TEM (HITACHI, H-7650, Japan), which showed their spheroid morphology and confirmed their size [18]. Flow cytometric characterization of exosomes: (FACS analysis) [19]. 1.…”
Section: Transmission Electron Microscope (Tem) Characterization For mentioning
confidence: 97%
“…Raw data for the MTT cell viability assay and for reverse transcription-quantitative PCR. DOI: 10.5256/ f1000research.14900.d203796 18 .…”
Section: Data Availabilitymentioning
confidence: 99%