Fibroblast Response to Hypoxia: The Relationship between Angiogenesis and Matrix Regulation

Potential role of heme oxygenase-1 in the progression of rat adjuvant arthritis

“…Hypoxia-driven angiogenesis is critical for extracellular matrix formation and remodeling in soft-tissue repair. 49 The inflamed synovium of rheumatoid arthritis shows a high level of angiogenesis 1 and increased Figure 8 Representative hind paw sections after TUNEL analysis of (a) arthritic control group and (b) SnPP-treated arthritic rats; original magnification  200. TUNEL analysis demonstrates decreased levels of programmed cell death in the treated rat compared to the control arthritic rats.…”

Provisional Matrix Deposition in Hemostasis and Venous Insufficiency: Tissue Preconditioning for Nonhealing Venous Ulcers

“…However, exposure to prolonged culture in hypoxic conditions ( > 1 week), thus, mimicking an ischemic dermal environment, did not support these processes. 112 Similarly, other studies using dermal fibroblasts have reported up-regulated expression of pro-a1 (I) 113 and (III) collagens 114,115 after acute exposure to hypoxic conditions in vitro, followed by decreased expression in response to prolonged hypoxia. 114,115 Under hypoxic conditions dermal fibroblastproduced collagen cannot be synthesized or crosslinked effectively, since the enzymes required for collagen polymerization and crosslinking, prolyl hydroxylase, lysyl hydroxylase, and lysyl oxidase, all require molecular oxygen as a cofactor to perform their biological function in the collagen synthesis and maturation pathway.…”

“…112 Similarly, other studies using dermal fibroblasts have reported up-regulated expression of pro-a1 (I) 113 and (III) collagens 114,115 after acute exposure to hypoxic conditions in vitro, followed by decreased expression in response to prolonged hypoxia. 114,115 Under hypoxic conditions dermal fibroblastproduced collagen cannot be synthesized or crosslinked effectively, since the enzymes required for collagen polymerization and crosslinking, prolyl hydroxylase, lysyl hydroxylase, and lysyl oxidase, all require molecular oxygen as a cofactor to perform their biological function in the collagen synthesis and maturation pathway. [116][117][118] More specifically, prolyl hydroxylase is required to convert proline residues to hydroxyproline, which allows the procollagen peptide chains to assume their triple helix configuration.…”

Biomechanical factors in Finite Element Analysis of abdominal aortic aneurysms

“…Aforementioned transformation includes: the inflammation and neovascularization [25], proteolytic activity [26] -especially increase in elastase production [27], decrease in collagen synthesis [28][29][30] and production of abnormal collagen [29]. From the clinical point of view, rapid growth and volume of ILT is correlated with the high risk of rupture [31].…”