2016
DOI: 10.1590/0103-6440201600748
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Fibroblast Viability after Storage at 20 °C in Milk, Hank's Balanced Salt Solution and Coconut Water

Abstract: The objective of this study was to evaluate the effectiveness of various storage media at 20 °C in maintaining the viability of human periodontal ligament fibroblasts (HPLF) over time. HPLF were maintained at 20 °C in skim milk (SM), whole milk (WM), freshly prepared Hank's balanced salt solution (HBSS), Save-A-Tooth(r), natural coconut water (NCW), coconut water industrialized (ICW) and tap water (negative control) for 3, 6, 24, 48, 72, 96 and 120 h. Cells maintained in Minimal Essential Medium (MEM-37) at 37… Show more

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Cited by 8 publications
(12 citation statements)
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“…Overall, 22 studies exclusively recommended natural products as storage media, while two studies recommended them in addition to HBSS and Ringer's lactate . A further three studies also recommend milk and HBSS along with different natural products .…”
Section: Resultsmentioning
confidence: 99%
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“…Overall, 22 studies exclusively recommended natural products as storage media, while two studies recommended them in addition to HBSS and Ringer's lactate . A further three studies also recommend milk and HBSS along with different natural products .…”
Section: Resultsmentioning
confidence: 99%
“…The most common reason for recommendation was based on the ability of the medium to maintain PDL cell viability, while a small number of recommendations were based on ease of availability, low cost, and long shelf life of the storage medium. Only a few media were recommended on the basis of actually enhancing the viability of PDL cells . The methods of assessing PDL cell viability were also evaluated, and Trypan blue staining was found to be the most frequently utilized laboratory technique to determine viable PDL cells (Figure ).…”
Section: Resultsmentioning
confidence: 99%
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