Fibroblasts Induce the Assembly of the Macromolecules of the Basement Membrane

Delvoye, P; Pierard, D.; Noël, Agnès; Nusgens, Betty; Foidart, Jean Michel; Lapière, Charles M.

doi:10.1111/1523-1747.ep12456042

Cited by 31 publications

(10 citation statements)

References 43 publications

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“…Alternatively, fibroblasts could reorganize the superficial dermis so as to stabilize previously assembled basement membrane. In this regard, it has been previously shown that fibroblasts were able to stabilize complexes of HSPG, laminin, and type IV collagen secreted by epithelial cells (Delvoye et al, 1988).…”

Section: Discussionmentioning

confidence: 96%

Cellular origin of the dermal‐epidermal basement membrane

Marinkovich

Keene

Rimberg

et al. 1993

Developmental Dynamics

231

158

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The basement membrane underlying epithelium of skin is generally believed to be of epithelial origin, but a mesenchymal contribution to the basement membrane has not been directly examined. The purpose of this study was to directly evaluate both epithelial and mesenchyma1 contributions to the basement membrane. Fetal bovine keratinocytes cultured on the surface of collagen gels in the absence of fibroblasts did not produce an ultrastructurally recognizable basement membrane; however, when these cells were cultured in the presence of dermal fibroblasts a basement membrane at the keratinocyte-fibroblast interface was produced after 1 week which was very similar in biochemical composition and ultrastructural appearance to dermal-epidermal basement membrane in human skin. When dual species cultures of bovine keratinocytes and human fibroblasts were analyzed by indirect immunofluorescent microscopy (IF), with human specific antibodies against basement membrane components, dermal fibroblasts were shown to synthesize and deposit type IV collagen, type VII collagen, and laminin in a linear manner into the basement membrane zone. Fetal bovine keratinocytes cultured in the presence or absence of fibroblasts synthesized and deposited type IV collagen, type VII collagen, laminin, K-laminin, kalinin, and basement membrane associated heparan sulfate proteoglycan (HSPG) into the underlying basement membrane zone. In organ culture, a subpopulation of fibroblasts initially migrating from human foreskin explants was found to stain strongly for types VII and IV collagen and laminin by IF whereas after subculture all cells showed a uniform low staining. Based on these observations we propose that differentiated fibroblasts exist adjacent to epithelial tissues in vivo which produce basement membrane components and assist in basement membrane assembly. 0 1993 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 96%

Cellular origin of the dermal‐epidermal basement membrane

Marinkovich

Keene

Rimberg

et al. 1993

Developmental Dynamics

231

158

View full text Add to dashboard Cite

show abstract

“…We have also recently shown that fibroblasts may induce the assembly of the macromolecules of the basement membrane (Delvoye et al, 1987). The phenotype of the cells may thus be expressed differently in the presence of occupied cell surface laminin or fibronectin receptors.…”

Section: Discussionmentioning

confidence: 99%

“…For example, normal hepatocytes prefer fibronectin, but when isolated from regenerating liver, they bind preferentially to laminin (Carlsson et al, 1981). We have also recently shown that fibroblasts may induce the assembly of the macromolecules of the basement membrane (Delvoye et al, 1987). The phenotype of the cells may thus be expressed differently in the presence of occupied cell surface laminin or fibronectin receptors.…”

Section: Discussionmentioning

confidence: 99%

Reconstituted basement‐membrane matrix modulates fibroblast activities in vitro

Emonard

Grimaud

Nusgens

et al. 1987

Journal Cellular Physiology

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Cellular growth and collagen biosynthesis were compared in dermal calf fibroblasts cultured on plastic or on a reconstituted basement membrane gel, termed matrigel. This matrix, extracted from Engelbreth-Holm-Swarm tumors, consists mainly of laminin, entactin, type IV collagen, and heparan sulfate proteoglycan. The multiplication rate of fibroblasts grown on matrigel was stimulated compared to that of monolayered cells cultured on plastic, and these cells formed multilayers after 4 days. Protein and collagen biosynthesis was reduced in fibroblasts cultured on matrigel. A higher proportion of the newly synthesized collagen (40%) was incorporated to the extracellular matrix in cultures grown on matrigel than in those grown on plastic (14%). Type III collagen was the preferential collagen type deposited on matrigel, and the ratio of type III:type I collagens secreted in the medium was also slightly higher in cultures grown on matrigel. Partially processed collagen was more abundant in fibroblasts grown on matrigel than in cells cultured on plastic. Finally, cells grown on matrigel exhibited a higher catabolic activity than cells grown on plastic. In this experimental model, the reconstituted basement-membrane matrix seems to influence the activities of fibroblasts significantly.

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“…The necessity of heterotypic cell contacts in vitro for the deposition of BM molecules in a polar fashion [see also [26][27][28][29], together with the finding of a dual origin of these mol ecules, emphasize the importance of a cellu lar cooperation for the elaboration of a pre cise extracellular microenvironment. In turn, these processes appear to be of prime importance in directing morphological changes of the cells and subsequent expres sion of differentiation markers.…”

Section: Elaboration Of a Basement Membrane In An In Vitro Epithelialmentioning

confidence: 99%

Synthesis of Basement Membrane Proteins in the Small Intestine

et al. 1990

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Are the basement membrane (BM) molecules involved in epithelial-mesenchymal cell interactions known to be instrumental in intestinal development and differentiation? Several findings argue in favor of this assumption. First, quantitative and/or qualitative changes in type IV collagen, laminin-nidogen and heparan sulfate proteoglycan (HSPG) are obvious at the phasis of intensive morphogenesis. Second, BM molecules deposited at the epithelial-mesenchymal interface are of dual origin: HSPG being produced by the epithelial cell population, while others like type IV collagen and laminin are mainly produced by the mesenchymal compartment. Third, the formation of the BM requires an actual contact between the epithelial and mesenchymal cells and always precedes the expression of differentiation markers in the epithelial cells. These data suggest that BM molecules display an instructive role in intestinal cell interactions.

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Fibroblasts Induce the Assembly of the Macromolecules of the Basement Membrane

Cited by 31 publications

References 43 publications

Cellular origin of the dermal‐epidermal basement membrane

Cellular origin of the dermal‐epidermal basement membrane

Reconstituted basement‐membrane matrix modulates fibroblast activities in vitro

Synthesis of Basement Membrane Proteins in the Small Intestine

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