Fibroblasts take the centre stage in human skeletal muscle regeneration

Mendias, Christopher L.

doi:10.1113/jp274403

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…However, the proper differentiation of MuSCs and maturation of myotubes into aligned myofibers follow restricted 3D spatially defined patterns 35,36 . Since FBs have been shown to support and sustain skeletal muscle regeneration [48][49][50] , we designed an experimental strategy in which HCC-Gel solution laden with both FBs and MuSCs was employed for testing whether i3D bioprinting of parallelepipeds could promote de novo skeletal muscle formation. A defined ratio 51 of GFP + MuSCs to mCherry + in HCC-Gel solution were injected and then i3D bioprinted into multiple parallelepiped-shaped constructs (Fig.…”

Section: In Vivo Spatially Controlled Cell Engraftmentmentioning

confidence: 99%

Intravital three-dimensional bioprinting

et al. 2020

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Despite the tremendous technical advancements in 3D bioprinting, the concept of fabricating 3D structures and functional tissues directly in live animals remains a visionary challenge. We show that 3D cell-laden hydrogels can be efficiently bioprinted across tissues and within tissues of living animals.We developed photo-sensitive polymers that allow in vitro and in vivo fabrication of hydrogels into pre-existing structures, by bio-orthogonal two-photon cycloaddition and crosslinking at wavelengths longer than 850 nm, without byproducts. By this technique, that we name intravital 3D bioprinting, after injection of these polymers in vivo it is possible to fabricate complex 3D structures inside tissues of living mice, including the dermis across epidermis, the skeletal muscle across epimysium or the brain across meninges. The use of commonly available multi-photon microscopes allows accurate (XYZ) positioning and orientation of bioprinted structures into specific anatomical sites. Finally, we show that intravital 3D bioprinting of donor muscle-derived stem cells allows de novo formation of myofibers in host animals. We envision that this strategy will offer an alternative in vivo approach to conventional bioprinting technology, holding great promises to substantially change the paradigm of 3D bioprinting for pre-clinical and clinical use.

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Section: In Vivo Spatially Controlled Cell Engraftmentmentioning

confidence: 99%

Intravital three-dimensional bioprinting

et al. 2020

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“…For instance, the presence of fibroblasts enhances myogenic cell differentiation and activity, whereas adipocytes can affect the process of energy consumption in myocytes by orchestrating the metabolite release and availability in the microenvironment. [385][386][387][388] Other cell types can be co-cultured with muscle cells in microfluidic systems such as endothelial cells and neurons. Endothelial cells are used to generate microvessel structures within the construct volume, which can then be perfused by microfluidic flows.…”

Section: Generation and Maturation Of Muscle Tissuementioning

confidence: 99%

Microfluidic Tissue Engineering and Bio‐Actuation

et al. 2022

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Bio‐hybrid technologies aim to replicate the unique capabilities of biological systems that could surpass advanced artificial technologies. Soft bio‐hybrid robots consist of synthetic and living materials and have the potential to self‐assemble, regenerate, work autonomously, and interact safely with other species and the environment. Cells require a sufficient exchange of nutrients and gases, which is guaranteed by convection and diffusive transport through liquid media. The functional development and long‐term survival of biological tissues in vitro can be improved by dynamic flow culture, but only microfluidic flow control can develop tissue with fine structuring and regulation at the microscale. Full control of tissue growth at the microscale will eventually lead to functional macroscale constructs, which are needed as the biological component of soft bio‐hybrid technologies. This review summarizes recent progress in microfluidic techniques to engineer biological tissues, focusing on the use of muscle cells for robotic bio‐actuation. Moreover, the instances in which bio‐actuation technologies greatly benefit from fusion with microfluidics are highlighted, which include: the microfabrication of matrices, biomimicry of cell microenvironments, tissue maturation, perfusion, and vascularization.

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“…ECM is the non-cellular, endomysial connective tissue that surrounds muscle structures [107,108]. ECM is created and repaired by fibroblasts which secrete ECM components, including collagen [109]. Different ECM compositions have been observed in individuals with and without CP and may account for both loss of function and loss of regenerative potential observed in CP.…”

Section: Muscle Cellular Physiology and Growthmentioning

confidence: 99%

Muscle architecture, growth, and biological Remodelling in cerebral palsy: a narrative review

Handsfield

Williams

Khuu

et al. 2022

BMC Musculoskelet Disord

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Cerebral palsy (CP) is caused by a static lesion to the brain occurring in utero or up to the first 2 years of life; it often manifests as musculoskeletal impairments and movement disorders including spasticity and contractures. Variable manifestation of the pathology across individuals, coupled with differing mechanics and treatments, leads to a heterogeneous collection of clinical phenotypes that affect muscles and individuals differently. Growth of muscles in CP deviates from typical development, evident as early as 15 months of age. Muscles in CP may be reduced in volume by as much as 40%, may be shorter in length, present longer tendons, and may have fewer sarcomeres in series that are overstretched compared to typical. Macroscale and functional deficits are likely mediated by dysfunction at the cellular level, which manifests as impaired growth. Within muscle fibres, satellite cells are decreased by as much as 40–70% and the regenerative capacity of remaining satellite cells appears compromised. Impaired muscle regeneration in CP is coupled with extracellular matrix expansion and increased pro-inflammatory gene expression; resultant muscles are smaller, stiffer, and weaker than typical muscle. These differences may contribute to individuals with CP participating in less physical activity, thus decreasing opportunities for mechanical loading, commencing a vicious cycle of muscle disuse and secondary sarcopenia. This narrative review describes the effects of CP on skeletal muscles encompassing substantive changes from whole muscle function to cell-level effects and the effects of common treatments. We discuss growth and mechanics of skeletal muscles in CP and propose areas where future work is needed to understand these interactions, particularly the link between neural insult and cell-level manifestation of CP.

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Fibroblasts take the centre stage in human skeletal muscle regeneration

Cited by 10 publications

References 5 publications

Intravital three-dimensional bioprinting

Intravital three-dimensional bioprinting

Microfluidic Tissue Engineering and Bio‐Actuation

Muscle architecture, growth, and biological Remodelling in cerebral palsy: a narrative review

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