Fibronectin adsorption, conformation, and orientation on polystyrene substrates studied by radiolabeling, XPS, and ToF SIMS

Lhoest, Jacques; Detrait, Eric; Aguilar, P van den Bosch de; Bertrand, Patrick

doi:10.1002/(sici)1097-4636(199807)41:1<95::aid-jbm12>3.0.co;2-g

Cited by 168 publications

(57 citation statements)

References 25 publications

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“…On the other hand, super-hydrophilic surface of titanium dioxide films 33 or O 2 -treated HMDSO films that showed a high rate of cell attachment had a rigid surface. It is also necessary to investigate the protein conformation that influences cell adhesion by using a TOF-SIMS system 29,30,34 for better understanding of these phenomena.…”

Section: Discussionmentioning

confidence: 99%

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

et al. 2006

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Surface wettability is an important physicochemical property of biomaterials, and it would be more helpful for understanding this property if a wide range of wettability are employed. This study focused on the effect of surface wettability on fibroblast adhesion over a wide range of wettability using a single material without changing surface topography. Plasma polymerization with hexa methyldisiloxane followed by oxygen (O 2 ) plasma treatment was employed to modify the surfaces. The water contact angle of sample surfaces varied from 106 degrees (hydrophobicity) to almost 0 degrees (super-hydrophilicity). O 2 -functional groups were introduced on polymer surfaces during O 2 -plasma treatment. The cell attachment study confirmed that the more hydrophilic the surface, the more fibroblasts adhered in the initial stage that includes on super-hydrophilic surfaces. Cells spread much more widely on the hydrophilic surfaces than on the hydrophobic surfaces. There was no significant difference in fibroblast proliferation, but cell spreading was much greater on the hydrophilic surfaces. These findings suggest the importance of the surface wettability of biomaterials on initial cell attachment and spreading. The degree of wettability should be taken into account when a new biomaterial is to be employed. Further research of surface wettability on adhesive molecules is necessary for a better understanding of this property.

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Section: Discussionmentioning

confidence: 99%

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

et al. 2006

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“…This is one of the bottleneck problems in the studies of protein adsorption. At present, there are many methods used for the measurements of protein adsorption, including radio-labeling [12] , antibody techniques [13,14] , QCM [15] , AFM [16,17] , the second mass spectra [18] , photoelectron spectroscopy analysis [19] , etc. However, until now, no any singular technology can overall analyze the protein adsorption process, including the categories and quantity of the adsorbed proteins, the conformational change after adsorption, and particularly the quantitative analysis of secondary structural change after adsorption.…”

Section: Discussionmentioning

confidence: 99%

Protein adsorption on the poly(L-lactic acid) surface modified by chitosan and its derivatives

Jiao

Zhou

et al. 2009

Chin. Sci. Bull.

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Surface modification of biomaterials has been adopted over the years to improve their biocompatibility. In this study, aiming to promote hydrophilicity and to introduce natural recognition sites onto poly(L-lactic acid) (PLLA) films, chitosan and its derivatives, carboxymethyl chitosan(CMC) and N-methylene phosphonic chitosan (NPC), were used to modify the surface of PLLA films by an entrapment method. Radiolabeled ( 125 I) proteins were used to measure the amount of protein adsorbed to PLLA surfaces. Fibronectin (Fn) was used to study the protein adsorption on the modified PLLA surfaces, including isotherm adsorption and adsorption kinetics of single protein, competitive adsorption of binary proteins system and serum multi-proteins and the desorption behavior in serum solution. The results showed that in the isotherm adsorption, Fn had a larger adsorption capacity on the CS-modified surface at lower concentrations, but had a high adsorption capacity at CMC-modified surface at higher concentrations. In the study of absorption kinetics, Fn had a fastest adsorption equilibrium and a highest equilibrium adsorption capacity at the CS-modified surface, while it was opposite at the PCS-modified surface. When BSA and serum were added, it had the greatest effect on the adsorption of Fn on the PCS-modified surface. After 6 hours soaking in the desorption study, Fn had reached desorption equilibrium on all the modified surfaces, which had different effects on the desorption rate and the remaining percentage of Fn.Reasonable responses of cells to the implant surface play a key role in tissue regeneration and integration. When biomaterials are implanted, they adsorb kinds of proteins from the surrounding blood and serum, which translate the signal of structure and composition of the surface to a biological "language". And cells can respond to the "language", but not the real material surface contacted, leading to of the final result of the interaction between implant materials and organization [1] .The quantity, density and conformation of the absorbed proteins mainly rely on the surface chemistry and physics performance of the materials. Protein adsorption is a very complex process, in which the interaction force includes Van der Waals force, hydrophobic interaction, electrostatic adsorption and hydrogen bonding, etc. The adsorption properties of proteins at solid/liquid interface can be affected by many factors, including the treatment process and measurement methods, etc. However, the chemical properties of material surface are the basis of protein adsorption. Although there are many researches about the protein adsorption and desorption, and there are also some specific models have been proposed [2][3][4][5] , the real mechanism is still not very clear. The effects of the interaction among the different proteins, the changes of protein structure and the dynamic changes of the protein conformation have effect on the adsorption properties in varying extent.Fibronectin (Fn), laminin (Ln), vitronectin (Vn) are the key member...

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“…The statistical method for the analysis of TOF-SIMS data was informed by the papers Lhoest et al (24,25) and Wagner et al (38,39). A statistical analysis was conducted to identify the molecules adsorbing from BALF to PM2.5.…”

Section: Pm2mentioning

confidence: 99%

Fine airborne urban particles (PM_2.5) sequester lung surfactant and amino acids from human lung lavage

Kendall¹

2007

American Journal of Physiology-Lung Cellular and Molecular Phys

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Kendall M. Fine airborne urban particles (PM2.5) sequester lung surfactant and amino acids from human lung lavage. Am J Physiol Lung Cell Mol Physiol 293: L1053-L1058, 2007. First published July 6, 2007; doi:10.1152/ajplung.00131.2007.-Components of surfactant act as opsonins and enhance phagocytosis of bacteria; whether this process occurs with atmospheric fine particles has not been shown. We have studied the interactions of fine particles (urban PM2.5) and surfactant removed from normal human lungs by lavage, using a surface analysis technique. The aim was to identify which of the chemical components of brochoalveolar lavage (BAL) deposit on the surfaces of urban PM 2.5. Deposition of surfactant components on urban PM2.5 surfaces was reported in previous studies, but molecular identification and relative quantification was not possible using simple data analysis. In this study, we were able to identify adsorbed components by applying an appropriate statistical technique, factor analysis. In this study, the most strongly associated mass fragment on PM2.5 surfaces exposed to BAL (and undetected on both untreated samples and saline controls) was di-palmitoyl-phosphatidylcholine, a component of lung surfactant. Amino acids were also strongly associated with BAL-exposed PM2.5 surfaces and not other sample types. Thirteen mass fragments were identified, diagnostic of the amino acids alanine, arginine, asparagine, aspartic acid, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, serine, and valine. This study provides evidence that lung surfactant and amino acids related to opsonin proteins adsorb to nonbiological particle surfaces exposed to human lung lining fluid. Disruption of normal surfactant function, both physical and immunological, is possible but unproven. Further work on this PM-opsonin interaction is recommended. bronchoalveolar lavage EPIDEMIOLOGICAL STUDIES have shown that exposure to particulate air pollutants damages health (32). The exact mechanisms further underlying these air pollution effects remain unknown, although recent work (29, 39) has shown that cardiovascular system (acutely) and the developing respiratory system (chronic effects; Ref. 11) are most significantly affected. Effects on the cardiovascular system were seen on the day of exposure, indicating immediate short-term effects that were detectable within hours of exposures. It is likely that sensitivity to particles varies across the population with the young, the elderly, and those with preexisting cardio-respiratory disease being most at risk (31). Effects occur at ambient concentrations in industrial countries: These concentrations are low compared with concentrations recorded in earlier periods and with those common, now, in developing countries (8). Epidemiological and toxicological studies have demonstrated associations between specific PM characteristics and increased mortality and morbidity. Implicated characteristics include surface area, metals, acidic components, oxidative stress potent...

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Fibronectin adsorption, conformation, and orientation on polystyrene substrates studied by radiolabeling, XPS, and ToF SIMS

Cited by 168 publications

References 25 publications

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

Protein adsorption on the poly(L-lactic acid) surface modified by chitosan and its derivatives

Fine airborne urban particles (PM_2.5) sequester lung surfactant and amino acids from human lung lavage

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