Fibronectin fixation on poly(ethyl acrylate)–based copolymers

Briz, Nerea; Antolinos-Turpín, Carmen M.; Alió, Jorge L.; Garagorri, Nerea; Ribelles, J.L. Gómez; Gómez-Tejedor, José A.

doi:10.1002/jbm.b.32907

Cited by 9 publications

(8 citation statements)

References 22 publications

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“…It has to be mentioned that a certain discrepancy was detected between the contact angle values here presented and the values found elsewhere [36]. These differences are to be attributed to the preparation of the substrates, being crosslinked films in the previously published results, and ultrathin spin coated films of non-crosslinked polymers in the present work.…”

Section: On the Acrylates Interaction With Rad16-icontrasting

confidence: 63%

Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

et al. 2016

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Self-assembling peptides (SAP) are widely used as scaffolds themselves, and recently as fillers of microporous scaffolds, where the former provides a cell-friendly nanoenvironment and the latter improves its mechanical properties. The characterization of the interaction between these short peptides and the scaffold material is crucial to assess the potential of such a combined system. In this work, the interaction between poly(ethyl acrylate) (PEA) and 90/10 ethyl acrylate-acrylic acid copolymer P(EAcoAAc) with the SAP RAD16-I has been followed using a bidimensional simplified model. By means of the techniques of choice (congo red staining, atomic force microscopy (AFM), and contact angle measurements) the interaction and self-assembly of the peptide has proven to be very sensitive to the wettability and electronegativity of the polymeric substrate.

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Section: On the Acrylates Interaction With Rad16-icontrasting

confidence: 63%

Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

et al. 2016

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“…For the production and purification of FNIII 7–10 , the pET‐11 plasmid containing the FNIII 7–10 sequence was transferred into Escherichia coli Bl21 (DE3; Invitrogen, Spain) and expressed as described elsewhere . The expressed proteins were entirely in the supernatant.…”

Section: Methodsmentioning

confidence: 99%

“…FN was covalently cross-linked to disks of different copolymers of EA [7][8][9][10] -coated surfaces. For the production and purification of FNIII 7-10 , the pET-11 plasmid containing the FNIII 7-10 sequence was transferred into E. coli Bl21 (DE3) (Invitrogen, Spain) and expressed as described elsewhere [14]. The expressed proteins were entirely in the supernatant.…”

Section: Preparation Of Fibronectin (Fn)-coated Surfacesmentioning

confidence: 99%

Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model

Barrio

Chiesa

Ferrer

et al. 2014

J Biomedical Materials Res

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“…A hidden RGD domain could lead to fewer binding events between the implant and capsule. 51 There is a debate about the specific role of fibronectin in PCO formation. According to Linnola et al, there is a strong link between the higher rate of fibronectin on implants and the lower incidence of PCO.…”

Section: F I G U R Ementioning

confidence: 99%

Comparative stability of intraocular lenses during 2–20 years of artificial aging, potential effects in terms of biocompatibility

Tortolano

Mrad

Manerlax

et al. 2022

J of Applied Polymer Sci

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Intraocular lenses (IOLs) are worldwide used for cataract surgery. Mostly made of acrylate polymers, they can lead to an unexpected biocompatibility reaction such as posterior capsular opacification (PCO). Acrylate polymers suffer from both oxidative and heat/thermal aging that affect their properties. We examined the stability of four currently available acrylic IOLs, three hydrophobic and one amphiphilic, under artificial aging that simulate intraocular conditions from 2 to 20 years. Released degradation products were detected by reversed‐phase high performance liquid chromatography. Thermal properties were investigated using thermogravimetric analysis, and differential scanning calorimetry measurements. Surface topography was analyzed by atomic force microscopy. The effect on biocompatibility was estimated with protein sorption of the aged lenses. Both surface and thermal properties changed under aging. Surface roughness of implants increased under aging in a kinetic in two steps. The aging also changed the polymer structure. These changes lead to a variable release of degradation products according to the chemical design of the copolymer. Glass temperature transition (Tg) and thermal degradation stability also suffered from aging. The aging has led to significant changes in protein sorption affinity to IOL surfaces. For pure hydrophobic acrylic copolymer, stability was higher for copolymers containing at least one fluoro methacrylate. The amphiphilic polymer appeared to have a time‐dependent aging that can affect the implant flexibility and could impact late biocompatibility reaction and tolerance.

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Fibronectin fixation on poly(ethyl acrylate)–based copolymers

Cited by 9 publications

References 22 publications

Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model

Comparative stability of intraocular lenses during 2–20 years of artificial aging, potential effects in terms of biocompatibility

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