Fibronectin Hyperpolarizes the Plasma Membrane Potential of Murine Erythroleukemia Cells

Arcangeli, Annarosa; Bene, Maria Riccarda Del; Ricupero, Letizia; Ballerini, Lara; Olivotto, Massimo

doi:10.1111/j.1749-6632.1988.tb22341.x

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…The Warburg effect and the connected metabolic crossways have been object of intensive investigations in our laboratory throughout the last forty years, initially employing the ascites hepatoma AH130 [ 4–7 ]. This highly anaplastic tumor was generated by treating Wistar rats with the carcinogen o- aminoazotoluene and has been widely used as in vivo model of experimental cancer [ 8,9 ]. After serial transplantations in rat peritoneal cavity, this tumor became composed of isolated spheroidal cells, fed by the ascites fluid extruded from the peritoneal vessels.…”

Section: The Aerobic Glycolysis and The Warburg's Effect: Dfinition Amentioning

confidence: 99%

The unveiling of the Warburg effect and the inscribed innovative approach to a radical non toxic anticancer therapy

et al. 2018

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The purpose of this research has been deciphering the Warburg paradox, the biochemical enigma unsolved since 1923. We solved it by demonstrating that its specific character, i.e. the forced aerobic lactate exportation, represents a crucial metabolic device to counteract the cytotoxic effect produced by an excess of pyruvate at the connection of glycolysis with the Krebs cycle. This solution was verified by exposing cancer cells of different histogenesis to pyruvate concentrations higher than the physiological ones, after showing that these concentrations are totally innocuous when injected into mice. The mechanism of the pyruvate cytotoxicity relies on the saturation of the respiratory chain, leading to a negative shift of the cytosolic NADP/NADPH ratio and the consequent restriction of the purine synthesis and the related cell apoptosis. The reducing equivalents generated by glycolysis and by cytosolic metabolism compete each other for their disposal trough the respiratory chain; this makes it that the cytotoxicity of pyruvate is inversely related to the mitochondrial number and efficiency of various cell types. Thus, the cytotoxicity is high in anaplastic cancer stem cells, whose mitochondria are extremely few and immature (cristae-poor); on the contrary, no inhibition is brought about in adult differentiated cells, physiologically rich of mature mitochondria. All this generates the pyruvate anticancer selectivity, together with the lack of a general toxicity, making pyruvate represent an ideal candidate for a radical non toxical anticancer treatment.

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Section: The Aerobic Glycolysis and The Warburg's Effect: Dfinition Amentioning

confidence: 99%

The unveiling of the Warburg effect and the inscribed innovative approach to a radical non toxic anticancer therapy

et al. 2018

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show abstract

Changes in Friend murine erythroleukaemia cell membranes during induced differentiation determined by electrorotation

Wang

Huang

Gascoyne

et al. 1994

Biochimica et Biophysica Acta (BBA) - Biomembranes

182

192

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Fibronectin-integrin binding promotes hyperpolarization of murine erythroleukemia cells

Arcangeli

Becchetti

Bene

et al. 1991

Biochemical and Biophysical Research Communications

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Fibronectin Hyperpolarizes the Plasma Membrane Potential of Murine Erythroleukemia Cells

Cited by 4 publications

References 6 publications

The unveiling of the Warburg effect and the inscribed innovative approach to a radical non toxic anticancer therapy

The unveiling of the Warburg effect and the inscribed innovative approach to a radical non toxic anticancer therapy

Changes in Friend murine erythroleukaemia cell membranes during induced differentiation determined by electrorotation

Fibronectin-integrin binding promotes hyperpolarization of murine erythroleukemia cells

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