Fibronectin induces capacitation-associated events through the endocannabinoid system in bull sperm

Osycka‐Salut, Claudia; Martínez-León, Eduardo; Gervasi, María Gracia; Castellano, Luciana; Davio, Carlos; Chiarante, Nicolás; Franchi, A.M.; Ribeiro, Maria L.C.; Díaz, Emilce S.; Pérez‐Martínez, Silvina

doi:10.1016/j.theriogenology.2020.04.031

Cited by 13 publications

(7 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed a larger number of strongly enriched GO terms for the catalog of genes mapping nearby the MN when compared to the SN fractions (Additional file 6 and 7). The most enriched terms for the MN fraction included sensory perception, which is important for sperm homeostasis (e.g., SOD2; [41]), capacitation (e.g., TRPV1; [42]) and chemotaxis guidance to the egg for fertilization (e.g., CA6; [43]) and cell differentiation, development and morphogenesis, all with obvious links to embryogenesis (Additional file 6).…”

Section: Resultsmentioning

confidence: 99%

Micrococcal nuclease sequencing of porcine sperm suggests a nucleosomal involvement on semen quality and early embryo development

Gòdia

Hammoud

Naval-Sánchez

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: The mammalian mature spermatozoon has a unique chromatin structure in which the vast majority of histones are replaced by protamines during spermatogenesis and a small fraction of nucleosomes are retained at specific locations of the genome. The chromatin structure of sperm remains unresolved in most livestock species, including the pig. However, its resolution could provide further light into the identification of the genomic regions related to sperm biology and embryo development and it could also help identifying molecular markers for sperm quality and fertility traits. Here, for the first time in swine, we performed Micrococcal Nuclease coupled with high throughput sequencing on pig sperm and characterized the mono-nucleosomal (MN) and sub-nucleosomal (SN) chromatin fractions. Results: We identified 25,293 and 4,239 peaks in the mono-nucleosomal and sub-nucleosomal fractions, covering 0.3% and 0.02% of the porcine genome, respectively. A cross-species comparison of nucleosome-associated DNAs in sperm revealed positional conservation of the nucleosome retention between human and pig. Gene ontology analysis of the genes mapping nearby the mono-nucleosomal peaks and identification of putative transcription factor binding motifs within the mono-nucleosomal peaks showed enrichment for sperm function and embryo development related processes. We found motif enrichment for the transcription factor Znf263, which in humans was suggested to be a key regulator of the genes with paternal preferential expression during early embryo development. Moreover, we found enriched co-occupancy between the RNAs present in pig sperm and the RNA related to sperm quality, and the mono-nucleosomal peaks. We also found preferential co-location between GWAS hits for semen quality in swine and the mono-nucleosomal sites identified in this study. Conclusions: These results suggest a clear relationship between nucleosome positioning in sperm and sperm and embryo development.

show abstract

Section: Resultsmentioning

confidence: 99%

Micrococcal nuclease sequencing of porcine sperm suggests a nucleosomal involvement on semen quality and early embryo development

Gòdia

Hammoud

Naval-Sánchez

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…On the other hand, the binding of fibronectin to α5β1 induces the release of a sperm population enriched in motile and capacitated spermatozoa in bovines [ 49 ]. This suggests that this molecular binding seems to be relevant for the communication of the sperm with the oviductal microenvironment and the induction of sperm capacitation.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of sperm integrin α5β1 as a potential marker of fertility in humans

et al. 2022

View full text Add to dashboard Cite

Sperm selection for assisted reproduction techniques is generally based on basic parameters, while key aspects of sperm competence and its journey from the deposition site to the fertilization site are overlooked. Consequently, identifying molecular markers in spermatozoa that can efficiently predict the fertility of a semen sample could be of great interest, particularly in cases of idiopathic male infertility. When spermatozoa reach the female reproductive tract, it provides to them the cellular and molecular microenvironment needed to acquire fertilizing ability. In this sense, considering the role that integrin α5β1 of spermatozoa plays in reproduction-related events, we investigated the correlation between the subcellular localization of sperm integrin α5β1 and early embryo development outcome after in vitro fertilization (IVF) procedures in human. Twenty-four semen samples from normozoospermic men and metaphase II (MII) oocytes from healthy women aged under 38 years, from couples who underwent IVF cycles, were used in this work. Sperm α5β1 localization was evaluated by immunofluorescence assay using an antibody against integrin α5 subunit. Integrin α5β1 was mainly localized in the sperm acrosomal region (45.33±7.89%) or the equatorial segment (30.12±7.43%). The early embryo development rate (data obtained from the Fertility Center) correlated positively with the localization of α5β1 in the acrosomal region (number of usable embryos / inseminated oocytes: ρ = 0.75; p<0.01 and number of usable embryos/total number of two pronuclear zygotes: ρ = 0.80; p<0.01). However, this correlation was not significant when the equatorial segment mark was evaluated. In addition, human sperm released from co-culture with bovine oviductal epithelial cells (BOEC) showed a significant enrichment in the acrosomal localization pattern of α5β1 compared to those sperm that were not co-cultured with BOEC (85.20±5.35% vs 35.00±17.09%, respectively, p<0.05). In conclusion, the evaluation of sperm integrin α5β1 immunolocalization could be a useful tool to select sperm with fertilizing ability from human semen samples before IVF procedures.

show abstract

“…Due to the interaction of sperm with the components of oviductal fluid, capacitation is initiated and the ability of sperm to bind to epithelial cells in the fallopian tube is gradually lost. It was discovered in cattle [35,42] that an increase of fibronectin levels in an oviductal fluid during the pre-ovulatory period may promote a sperm release from the oviductal reservoir through the interaction with the sperm surface fibronectin receptor α5β1 integrin.…”

Section: Integrins As a Part Of The Oviductal Reservoir Of Spermmentioning

confidence: 99%

Role of Integrins in Sperm Activation and Fertilization

Merc

Frolikova

Komrsková

2021

IJMS

View full text Add to dashboard Cite

In mammals, integrins are heterodimeric transmembrane glycoproteins that represent a large group of cell adhesion receptors involved in cell–cell, cell–extracellular matrix, and cell–pathogen interactions. Integrin receptors are an important part of signalization pathways and have an ability to transmit signals into and out of cells and participate in cell activation. In addition to somatic cells, integrins have also been detected on germ cells and are known to play a crucial role in complex gamete-specific physiological events, resulting in sperm-oocyte fusion. The main aim of this review is to summarize the current knowledge on integrins in reproduction and deliver novel perspectives and graphical interpretations presenting integrin subunits localization and their dynamic relocation during sperm maturation in comparison to the oocyte. A significant part of this review is devoted to discussing the existing view of the role of integrins during sperm migration through the female reproductive tract; oviductal reservoir formation; sperm maturation processes ensuing capacitation and the acrosome reaction, and their direct and indirect involvement in gamete membrane adhesion and fusion leading to fertilization.

show abstract

Fibronectin induces capacitation-associated events through the endocannabinoid system in bull sperm

Cited by 13 publications

References 51 publications

Micrococcal nuclease sequencing of porcine sperm suggests a nucleosomal involvement on semen quality and early embryo development

Micrococcal nuclease sequencing of porcine sperm suggests a nucleosomal involvement on semen quality and early embryo development

Evaluation of sperm integrin α5β1 as a potential marker of fertility in humans

Role of Integrins in Sperm Activation and Fertilization

Contact Info

Product

Resources

About