Fibronectin involvement in granulation tissue and wound healing in rabbits.

Repesh, L A; Fitzgerald, Thomas J.; Furcht, Leo T.

doi:10.1177/30.4.6174568

Cited by 120 publications

(39 citation statements)

References 27 publications

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“…Repesh and co-workers [31] described an intense fibronectin staining in 8-day-old granulation tissue in rabbits which could also be detected by us in granulation tissue of skin wounds with an age of less than 17 days, especially less than 3 weeks.…”

Section: Discussionsupporting

confidence: 72%

“…Clark and co-workers [7] investigated the role of fibronectin during neoangiogenesis in granulation tissue and reported a reduction of this glycoprotein in the capillary wall with decreasing capillary ingrowth. Repesh et al [31] found fibronectin in the fibrin clot and described an intense reaction in 8-day-old granulation tissue from rabbits. Viljanto et al [37] detected fibronectin at the margin of cellulose compresses in 3-hour-old surgical wounds.…”

Section: Discussionmentioning

confidence: 98%

“…The intimate interaction between fibronectin and fibrin renders it feasible that this glycoprotein could be detected in early phases of wound healing [13,31]. On the other hand it is reasonable to assume that the fibronectin staining is reduced in the lesion area when cell migration into the wound area decreases [8].…”

Section: Discussionmentioning

confidence: 99%

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Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds

et al. 1992

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Summary:We analyzed the distribution of fibronectin in routinely embedded tissue specimens from 53 skin wounds and 6 postmortem wounds. In postmortem wounds a faint but focal positive staining was exclusively found at the margin of the specimens which dit not extend into the adjacent stroma. Vital wounds were classified into 3 groups. The first comprising lesions with wound ages ranging from a few seconds to 30min, the second comprising those with wound ages upt to 3 weeks, and the third group with lesions more than 3 weeks old. Ten out of 17 lesions with a wound age up to 30 rain showed a clear positive reaction within the wound area. Three specimens in this group were completely negative, while in 4 additional cases the result was not significantly different from postmortem lesions. These 7 Cases were characterized by acute death with extremely short survival times (only seconds). In wounds up to 3 weeks old fibronectin formed a distinct network containing an increasing number of inflammatory cells corresponding to the wound age. In 2 cases with a survival time of 17 days and in all wounds older than 3 weeks fibronectin was restricted to the surface of fibroblasts and to parallel arranged fibers in the granulation tissue without any network structures. We present evidence that fibronectin is a useful marker for vital wounds with a survival time of more than a few minutes. Fibronectin appears before neutrophilic granulocytes migrate into the wound area. Since a faint positive fibronectin staining is seen in postmortem lesions and bleedings, we propose that only those wounds which show strong positive fibronectin staining also extending into the adjacent stroma should be regarded as vital.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 98%

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Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds

et al. 1992

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“…Fibronectin has been proposed as a mediator of granulation tissue formation and wound healing (Repesh et al, 1982). The staining of vascular basement membrane in the tumours is also consistent with that in wound healing (Clark et al, 1982) and the possibility that the stroma of tumours is the same type of tissue as that of a healing wound should be considered, a concept which has been suggested in the case of breast cancer (Dvorak et al, 1981).…”

Section: Discussionmentioning

confidence: 97%

Laminin and fibronectin in rectal adenocarcinoma: Relationship to tumour grade, stage and metastasis

Förster¹,

Talbot²,

Critchley³

1984

Br J Cancer

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Summary Using an immunoperoxidase procedure, we have examined the distribution of laminin and fibronectin in normal human large intestinal mucosa and in 50 cases of rectal adenocarcinoma for which extensive clinical follow up was available. In normal tissue, laminin staining was largely restricted to basement membranes, including that underlying the epithelial cells, whereas fibronectin was found in both basement membranes and surrounding connective tissue.In rectal carcinomas, basement membrane-like staining for laminin associated with tumour cells was found in only 27 out of the 50 cases studied. Statistical analysis showed that the presence of laminin-containing basement membranes was correlated with low histological grade (well-differentiated tumours), but not with stage (progression through the bowel wall and the development of lymph node metastases) and, in a highly significant way, with a reduced incidence of distant metastases and increased patient survival.Although fibronectin was found in tumour cell basement membranes where these were present, it was also found in the stroma of all 50 tumours. There was no apparent correlation between the presence of stromal fibronectin and grade, stage or development of metastases.

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“…Increased levels of plasma fibronectin under acute phase conditions might facilitate the removal of microaggregates, bacteria, and soluble debris from the circulation and, thus improve the function of the reticulo-endothelial System (56,57). Furthermore, acute phase fibronectin might be deposited in the tissue at sites of acute inflammation and injury and thereby support wound healing and tissue repair (58,59). Stimulated synthesis of fibronectin in hepatocytes would therefore compensate for its increased consumption under these conditions.…”

Section: Discussionmentioning

confidence: 99%

Fibronectin is Synthesized as an Acute Phase Reactant in Rat Hepatocytes

Pick-Kober¹,

Münker²,

Gressner³

1986

Clinical Chemistry and Laboratory Medicine

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Summary: Elevated concentrations of fibronectin were found in plasma of rats under different acute phase conditions. Untreated animals showed a plasma fibronectin concentration of 150 + 50 mg/1, which increased to 412 ± 59 mg/1 24 h after subcutaneous injection of turpentine. The time course of the changes in plasma fibronectin concentration showed a peak at 24 h and a decline to norinal concentrations 72 h after turpentine treatment. Additional Stimulation by dexamethasone resulted in plasma fibronectin concentrations of 661 ± 49 mg/1. No or only slight elevations of fibronectin concentrations were observed after treatment with adrenaline, thyroxine and triiodothyronine äs compared with saline-injected animals. The common identity of plasma fibronectin in Controls, turpentine and turpentine-dexamethasone-treated animals was shown by slab gel electrophoresis under nonreducing conditions, followed by western blot and immunofluorescence staining. One dimensional immunoelectrophoresis performed with polyclonal antibodies to human fibronectin cross-reacting with rat fibronectin (shown by Ouchterlony gel diffusion) revealed indentical precipitation lines for the plasma of control and acute phase animals. Hepatocytes of turpentine-pretreated rats show a threefold increase of [ 14 C]valine incoiporation into total protein and a fourfold increase of immunoreactive radioactively labeled fibronectin in the culture medium, compared with control hepatocyte cultures. These results point to the role of hepatocytes in the synthesis of plasma fibronectin, which behaves in rats äs an acute phase reactant.

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Fibronectin involvement in granulation tissue and wound healing in rabbits.

Cited by 120 publications

References 27 publications

Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds

Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds

Laminin and fibronectin in rectal adenocarcinoma: Relationship to tumour grade, stage and metastasis

Fibronectin is Synthesized as an Acute Phase Reactant in Rat Hepatocytes

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