Fictibacillus halophilus sp. nov., from a microbial mat of a hot spring atop the Himalayan Range

Sharma, Anukriti; Kohli, Puneet; Singh, Yogendra; Schümann, Peter; Lal, Rup

doi:10.1099/ijsem.0.001051

Cited by 24 publications

(4 citation statements)

References 40 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the strains isolated from algal bodies, Bacillota dominated at the phylum level, which was consistent with the results of studies on terrestrial plants (Webster et al, 2020). However, the results at the genus level and species were different from those in terrestrial plants, with most of the detected bacteria being saline-alkali tolerant or salt tolerant bacteria (Supplementary Table 1), such as A. algicola (Ivanova et al, 2004), A. berkeleyi (Patel and Gupta, 2020), A. caeni (Patel and Gupta, 2020), R. aquimaris (Wang et al, 2023), F. halophilus (Sharma et al, 2016), H. faecis (Zhang et al, 2019), and H. kuroshimensis (Shi et al, 2020), indicating the adaptation of algal endophytic bacteria to the marine environment.…”

Section: Composition Of the Endophytic Bacterial Community In S Thunb...supporting

confidence: 85%

The host sex contributes to the endophytic bacterial community in Sargassum thunbergii and their receptacles

Zhao,

Sun,

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Endophytic bacteria have a complex coevolutionary relationship with their host macroalgae. Dioecious macroalgae are important producers in marine ecosystems, but there is still a lack of research on how sex influences their endophytic bacteria. In this study, the endophytic bacterial communities in male and female S. thunbergii and their reproductive tissues (receptacles) were compared using culture methods and high-throughput sequencing. The endophytic bacterial communities detected by the two methods were different. Among the 78 isolated strains, the dominant phylum, genus, and species were Bacillota, Alkalihalobacillus, and Alkalihalobacillus algicola, respectively, in the algal bodies, while in the receptacles, they were Bacillota, Vibrio, and Vibrio alginolyticus. However, 24 phyla and 349 genera of endophytic bacteria were identified by high-throughput sequencing, and the dominant phylum and genus were Pseudomonadota and Sva0996_ Marine_ Group, respectively, in both the algal body and the receptacles. The two methods showed similar compositions of endophytic bacterial communities between the samples of different sexes, but the relative abundances of dominant and specific taxa were different. The high-throughput sequencing results showed more clearly that the sex of the host alga had an effect on its endophyte community assembly and a greater effect on the endophytic bacterial community in the receptacles. Moreover, most specific bacteria and predicted functional genes that differed between the samples from the males and females were related to metabolism, suggesting that metabolic differences are the main causes of sex differences in the endophytic bacterial community. Our research is the first to show that host sex contributes to the composition of endophytic bacterial communities in dioecious marine macroalgae. The results enrich the database of endophytic bacteria of dioecious marine macroalgae and pave the way for better understanding the assembly mechanism of the endophytic bacterial community of algae.

show abstract

Section: Composition Of the Endophytic Bacterial Community In S Thunb...supporting

confidence: 85%

The host sex contributes to the endophytic bacterial community in Sargassum thunbergii and their receptacles

Zhao,

Sun,

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The genus Altererythrobacter , belonging to the phylum Proteobacteria , has been isolated from various marine and terrestrial environments such as deep-sea water [46], tidal flats [47], marine sediments [48], forests, desert soil [49, 50], hot springs [51], and the air [52]. The genus Fictibacillus , belonging to the phylum Firmicutes , has been previously isolated from different environments, including hot springs [53], industrial wastes [54], metal ores [55], and marine sediments [56]. Our study increased the knowledge of the diversity of available ascidian-derived microorganisms, providing new resources useful for the screening of novel strains and bioactive compounds.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis and Screening of Antimicrobial and Antiproliferative Activities of Culturable Bacteria Associated with the AscidianStyela clavafrom the Yellow Sea, China

Chen

Wang

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

Over 1,000 compounds, including ecteinascidin-743 and didemnin B, have been isolated from ascidians, with most having bioactive properties such as antimicrobial, antitumor, and enzyme-inhibiting activities. In recent years, direct and indirect evidence has shown that some bioactive compounds isolated from ascidians are not produced by ascidians themselves but by their symbiotic microorganisms. Isolated culturable bacteria associated with ascidians and investigating their potential bioactivity are an important approach for discovering novel compounds. In this study, a total of 269 bacteria were isolated from the ascidian Styela clava collected from the coast of Weihai in the north of the Yellow Sea, China. Phylogenetic relationships among 183 isolates were determined using their 16S rRNA gene sequences. Isolates were tested for antimicrobial activity against seven indicator strains, and an antiproliferative activity assay was performed to test for inhibition of human hepatocellular carcinoma Bel 7402 and human cervical carcinoma HeLa cell proliferation. Our results showed that the isolates belonged to 26 genera from 18 families in four phyla (Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes). Bacillus and Streptomyces were the most dominant genera; 146 strains had potent antimicrobial activities and inhibited at least one of the indicator strains. Crude extracts from 29 strains showed antiproliferative activity against Bel 7402 cells with IC50 values below 500 μg·mL−1, and 53 strains showed antiproliferative activity against HeLa cells, with IC50 values less than 500 μg·mL−1. Our results suggest that culturable bacteria associated with the ascidian Styela clava may be a promising source of novel bioactive compounds.

show abstract

“…In the past, the sequence-based viral characterization as well as electron and ion microscopy have been used for elucidating viral diversity within geothermally heated ecosystems (Häring et al, 2005). A series of Himalayan hot springs (surface temperature > 95°C) located at an altitude of 1,760 m at Manikaran (32°02′N, 72°21′E) have previously been investigated using sequencing approaches to characterize the bacterial communities (Dwivedi et al, 2012; Mahato et al, 2014; Sharma et al, 2014, 2016b; Tripathi et al, 2016). Culture-independent analysis of the sediment and microbial mat samples demonstrated unexpectedly high microbial diversity in these hot springs (Sangwan et al, 2015; Sharma et al, 2016a).…”

Section: Introductionmentioning

confidence: 99%

Bacterial and Archaeal Viruses of Himalayan Hot Springs at Manikaran Modulate Host Genomes

et al. 2018

Self Cite

View full text Add to dashboard Cite

Hot spring-associated viruses, particularly the archaeal viruses, remain under-examined compared to bacteriophages. Previous metagenomic studies of the Manikaran hot springs in India suggested an abundance of viral DNA, which prompted us to examine the virus–host (bacterial and archaeal) interactions in sediment and microbial mat samples collected from the thermal discharges. Here, we characterize the viruses (both bacterial and archaeal) from this Himalayan hot spring using both metagenomics assembly and electron microscopy. We utilized four shotgun samples from sediment (78–98°C) and two from microbial mats (50°C) to reconstruct 65 bacteriophage genomes (24–200 kb). We also identified 59 archaeal viruses that were notably abundant across the sediment samples. Whole-genome analyses of the reconstructed bacteriophage genomes revealed greater genomic conservation in sediments (65%) compared to microbial mats (49%). However, a minimal phage genome was still maintained across both sediment and microbial mats suggesting a common origin. To complement the metagenomic data, scanning-electron and helium-ion microscopy were used to reveal diverse morphotypes of Caudovirales and archaeal viruses. The genome level annotations provide further evidence for gene-level exchange between virus and host in these hot springs, and augments our knowledgebase for bacteriophages, archaeal viruses and Clustered Regularly Interspaced Short Palindromic Repeat cassettes, which provide a critical resource for studying viromes in extreme natural environments.

show abstract

Fictibacillus halophilus sp. nov., from a microbial mat of a hot spring atop the Himalayan Range

Cited by 24 publications

References 40 publications

The host sex contributes to the endophytic bacterial community in Sargassum thunbergii and their receptacles

The host sex contributes to the endophytic bacterial community in Sargassum thunbergii and their receptacles

Phylogenetic Analysis and Screening of Antimicrobial and Antiproliferative Activities of Culturable Bacteria Associated with the AscidianStyela clavafrom the Yellow Sea, China

Bacterial and Archaeal Viruses of Himalayan Hot Springs at Manikaran Modulate Host Genomes

Contact Info

Product

Resources

About