Field and Laboratory Methods

Degma, Peter

doi:10.1007/978-3-319-95702-9_14

Cited by 14 publications

(7 citation statements)

References 148 publications

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“…Tardigrades and rotifers were mounted on microscope slides in a small drop of Hoyer’s medium 49 or in drop of water then examined under the following light microscopes BX51 and Olympus BX53 with phase-contrast (PCM) and another with differential interference contrast (DIC). Pictures were taken using a DP21 digital camera, cellSens Entry 1.12 software (Olympus, Tokyo, Japan) or Quick PHOTO CAMERA 3.0 software (Promicra, Prague, Czech Republic).…”

Section: Methodsmentioning

confidence: 99%

Snow algae blooms are beneficial for microinvertebrates assemblages (Tardigrada and Rotifera) on seasonal snow patches in Japan

Ono

Takeuchi

Zawierucha

2021

Sci Rep

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Although studies on snow algae and macroinvertebrates have been frequently conducted on snow patches, only few surveys have been focused on microinvertebrates which reach high biomass and play various trophic roles in other cold habitats. The aims of this study were (1) to search for microinvertebrates in seasonal surface snow patches located on the slope of Mt. Gassan, in northern Japan, and (2) to identify factors determining their distribution associated with snow algal blooms of various colorations (orange, green, and golden-brown) collected from the same sampling site over two seasons (2018, 2019). Microscopic observation revealed presence of two major groups of microinvertebrates: Tardigrada and Rotifera. They were concentrated in green snow colored by blooms of Chloromonas sp. in comparison to orange or golden-brown snow and only a few were found in white snow. Mean body length of tardigrades increased throughout the melt season, their intestine content was green and they laid eggs on colored snow. These results suggest that tardigrades preferentially grew and reproduced on green snow patches. Population densities of tardigrades, rotifers and concentration of chlorophyll a were significantly correlated. Our study indicates that green snow patches in temperate mountainous forests constitute important and unique low-temperature ecosystems for microinvertebrates. Snow covered by algae is an unrecognized novel habitats for tardigrades and rotifers.

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Section: Methodsmentioning

confidence: 99%

Snow algae blooms are beneficial for microinvertebrates assemblages (Tardigrada and Rotifera) on seasonal snow patches in Japan

Ono

Takeuchi

Zawierucha

2021

Sci Rep

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“…It is also recommended to test various alignment strategies and tree-building methods to ensure the robustness of phylogenetic trees. Hologenophores (i.e., specimens that first served for morphological and then for molecular analyses) constitute an essential link between sequence data and their taxonomic origin, providing a means to verify the taxonomic identity of the specimens sequenced (Pleijel et al 2008;Degma 2018). Finally, the taxon sampling should be comprehensive and balanced, which applies also to morphological analyses.…”

Section: Morphology and Molecules In Systematics And Taxonomymentioning

confidence: 99%

A huge undescribed diversity of the subgenus Hystricochaetonotus (Gastrotricha, Chaetonotidae, Chaetonotus) in Central Europe

Križanová

Vďačný

2022

EJT

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The subgenus Hystricochaetonotus Schwank, 1990 is one of the most species-rich subgenera of Chaetonotus Ehrenberg, 1830. It has a worldwide distribution and encompasses 37 species predominantly living in the benthos and periphyton of limnetic habitats. We have discovered further nine new species in running and stagnant waters in Slovakia (Central Europe): Ch. (H.) arcanus sp. nov., Ch. (H.) avarus sp. nov., Ch. (H.) gulosus sp. nov., Ch. (H.) iratus sp. nov., Ch. (H.) luxus sp. nov., Ch. (H.) mirabilis sp. nov., Ch. (H.) optabilis sp. nov., Ch. (H.) slavicus sp. nov., and Ch. (H.) superbus sp. nov. Their morphology was studied using differential interference contrast microscopy and subsequent morphometric analyses were carried out. In addition, the primary and secondary structures of their 18S, ITS2, and 28S rRNA molecules as well as their barcoding mitochondrial gene encoding for cytochrome c oxidase (COI) were analyzed. Species boundaries were tested also using the compensatory base change analysis. The new species could be well separated both morphologically and molecularly. The present barcoding analyses revealed that the nuclear ITS2 sequences represent a powerful DNA barcode in addition to the mitochondrial COI gene. According to the multi-gene phylogenetic analyses, the lineage leading to the last common ancestor of the ‘Hystricochaetonotus’ clade is the longest internal branch within the family Chaetonotidae Gosse, 1864. Since members of the subgenus Hystricochaetonotus are morphologically highly heterogeneous, parallel evolution of Chaetonotus-like and/or Hystricochaetonotus-like characters of scales and spines occurred during its radiation.

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“…Recent estimates augment the increasing evidence for the existence of numerous species complexes (Faurby et al 2012;Jørgensen et al 2018;Guidetti et al 2019;Morek and Michalczyk 2020), however, rather mediocre species richness of this phylum emerges when compared to other animal groups characterised by greater species abundance by an order of magnitude (Bartels et al 2016). Many regions of the world have never been sampled in a search for tardigrades, although the collection of these animals is very easy and not costly (Degma 2018).…”

Section: Introductionmentioning

confidence: 96%

Echiniscidae in the Mascarenes: the wonders of Mauritius

Kiosya¹,

Vončina²,

Gąsiorek³

2021

EvolSyst

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Many regions of the world remain unexplored in terms of the tardigrade diversity, and the islands of the Indian Ocean are no exception. In this work, we report four species of the family Echiniscidae representing three genera from Mauritius, the second largest island in the Mascarene Archipelago. Two species belong in the genus Echiniscus: Echiniscus perarmatus Murray, 1907, a pantropical species, and one new species: Echiniscus insularissp. nov., one of the smallest members of the spinulosus group and the entire genus, being particularly interesting due to the presence of males and supernumerary teeth-like spicules along the margins of the dorsal plates. The new species most closely resembles Echiniscus tropicalis Binda & Pilato, 1995, for which we present extensive multipopulation data and greatly extend its distribution eastwards towards islands of Southeast Asia. Pseudechiniscus (Meridioniscus) mascarenensissp. nov. is a typical member of the subgenus with elongated (dactyloid) cephalic papillae and the pseudosegmental plate IV’ with reduced posterior projections in males. Finally, a Bryodelphax specimen is also recorded. The assemblage of both presumably endemic and widely distributed tardigrade species in Mauritius fits the recent emerging biogeographic patterns for this group of micrometazoans.

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Field and Laboratory Methods

Cited by 14 publications

References 148 publications

Snow algae blooms are beneficial for microinvertebrates assemblages (Tardigrada and Rotifera) on seasonal snow patches in Japan

Snow algae blooms are beneficial for microinvertebrates assemblages (Tardigrada and Rotifera) on seasonal snow patches in Japan

A huge undescribed diversity of the subgenus Hystricochaetonotus (Gastrotricha, Chaetonotidae, Chaetonotus) in Central Europe

Echiniscidae in the Mascarenes: the wonders of Mauritius

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