FIELD APPLICATION FOR EXPERIMENTAL INACTIVATED MULTI-VALENT P. multocida AND AVIAN INFLUENZA (H9N2) VACCINE IN POULTRY

Abstract

Fowl cholera (FC) and Avian Influenza (AI) are two of the major economically important respiratory and septicaemic disease of poultry in Egypt and allover the world. A field trails was conducted to evaluate the immunogenicity of an experimentally multivalent inactivated Fowl cholera (P. multocida) and Avian Influenza (H9N2) vaccine. The In this study a combined inactivated Montanide ISA fowl cholera and AI vaccine was prepared then the immunization potency and protective efficacy were evaluated through experimental application on different poultry breeds including broiler breeder, broiler and turkey flocks.The peak of humeral immune response against P. multocida as measured by ELISA was at the 5th week post vaccination and at 3rd week post boostering all over the used poultry flocks. At the same time, AI immune response as measured by HI reached the peak at the 6 th week post vaccination and at 2 nd week post boostering. Regarding the challenge test, the protection levels were 76.6, 70 and 80% and 90, 86.6 and 93.3% against P. multocida while protection levels were 83.3, 80 and 86.6% and 93.3, 90 and 96.6% against AI virus after challenge with the used virulent strains post single and booster dose respectively. In conclusion the combined prepared fowl cholera and AI vaccine succeeded in eliciting protective antibody titres and full protection against both fowl cholera and AI diseases.

Trials for preparation and evaluation of a combined inactivated reassorted H5N1 and Escherichia coli O157 vaccine in poultry

“…The two vaccines in Groups 1-3 had protection rates of 80-100%. These results agree with those of Salama et al [19] who found protection levels of 80-93.3% against Pasteurella multocida and 96.6% against AI virus after challenging with the used virulent strains after the single and booster doses, respectively. Moreover, Ghanem et al [20] found a high degree of protection for layers and chicks with a vaccine prepared from trivalent E. coli (serotypes O1, O2, and O78) and an inactivated Newcastle disease.…”

“…In an investigation of HI and ELISA tests of the sera of chickens vaccinated with combined AI (H9N2) and fowl cholera (FC), the study by Salama et al . [ 19 ] indicated similar results until week 6 post-vaccination for FC using Montanide ISA206. In addition, Ghanem et al .…”

“…In our experimental trials, the chickens vaccinated with combined inactivated E. coli and AI gave a higher immune response at week 7 than those vaccinated with the inactivated E. coli. In an investigation of HI and ELISA tests of the sera of chickens vaccinated with combined AI (H9N2) and fowl cholera (FC), the study by Salama et al [19] indicated similar results until week 6 post-vaccination for FC using Montanide ISA206. In addition, Ghanem et al [20] have stated that using Montanide ISA70 as an adjuvant in preparation of bivalent combined vaccine (AI + FC), monovalent AI vaccine and monovalent FC vaccine gave better post-vaccine reaction and higher immune response than using white oil adjuvant.…”

“…These results agree with those of Salama et al . [ 19 ] who found protection levels of 80-93.3% against Pasteurella multocida and 96.6% against AI virus after challenging with the used virulent strains after the single and booster doses, respectively. Moreover, Ghanem et al .…”

Imunitas Ayam Petelur Pascavaksinasi dengan Vaksin Flu Burung Subtipe H9N2

“…Berdasarkan hasil analisis titer antibodi pascavaksinasi menunjukkan terjadi peningkatan titer antibodi setiap minggu pada kelompok P1 dan P2 dengan rerata titer antibodi kelompok P2 lebih tinggi dibandingkan titer antibodi kelompok kontrol dan P1. Temuan ini sesuai dengan laporan Salama et al (2019) yang menyatakan bahwa inokulasi dosis booster vaksin H9N2 menyebabkan peningkatan titer antibodi yang cepat, dengan puncak titer antibodi AI ditemukan pada minggu ke-3 pasca boostering. Pada vaksinasi ulangan, titer antibodi yang dihasilkan relatif lebih tinggi dan pembentukannya juga lebih cepat dibandingkan dengan vaksinasi pertama.…”