Field desorption mass spectrometry: Applications

Wood, Gordon

doi:10.1002/mas.1280010106

Cited by 42 publications

(8 citation statements)

References 87 publications

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“…'2 Bacterial phospholipids have been profiled utilizing negative ion FAB mass spectrometry and constant neutral loss scanning for FAB mass spectrometry by Heller et aI.'3.' 4 We have reported the use of FAB mass spectrometry in the analysis of mixtures of intact, naturally occurring bacterial phospho1ipids.l We now present further details of these results and provide highresolution FAB and tandem mass spectral data for further confirmation of structural assignments. Our results indicate that FAB mass spectrometry is a useful method for the analysis of bacterial phospholipids in mixtures and provides information useful in bacterial identification and taxonomy.…”

Section: Introductionmentioning

confidence: 86%

Bacterial phospholipid analysis by fast atom bombardment mass spectrometry

Pramanik¹,

Zechman²,

Das³

et al. 1990

Biol. Mass Spectrom.

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The structural analysis of naturally occurring bacterial phospholipids in mixtures by fast atom bombardment (FAB) mass spectrometry are reported. The bacterial strains examined included several genera of actinomycetes, two strains of Escherichia coli, and one strain each of Proteus mirabilis and Pseudomonas aeruginosa. FAB mass spectrometry proved to be a useful tool for the structural identification of phospholipids in mixtures and provided stable pseudo-molecular ions and characteristic fragment ions which permitted the identification of phosphatidylethanolamine and phosphatidyl choline. Information regarding the chain length of the fatty acids, their degree of unsaturation in the chains and the presence of hydroxyl groups was also obtained. The results obtained by FAB mass spectrometry were supported by high-resolution mass spectral data, tandem mass spectrometric studies and FAB mass spectrometry of components which had been separated and partially purified by thin-layer chromatography. Each organism displayed a highly characteristic phospholipid profile suggesting the possible use of FAB mass spectrometry as a method for rapid bacterial detection and identification.

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Section: Introductionmentioning

confidence: 86%

Bacterial phospholipid analysis by fast atom bombardment mass spectrometry

Pramanik¹,

Zechman²,

Das³

et al. 1990

Biol. Mass Spectrom.

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“…Instead it may as well be applied to ionic analytes, i.e., organic salts. 20,[27][28][29][30][31][32][33][34][35][36][37][38][39] The flourishing period of FD-MS began in the mid-1970s, which led to Beckey's second monograph now covering FI-MS and FD-MS. 31 From the mid-1980s, the strong competition from the easier-to-operate fast-atom bombardment (FAB) [40][41][42] caused a decrease in applications of FD-MS. In the mid and late 1980s even more competition arrived by the advent of electrospray ionization (ESI) 43,44 and matrix-assisted laser desorption/ionization (MALDI).…”

Section: Historical Sketchmentioning

confidence: 99%

From the discovery of field ionization to field desorption and liquid injection field desorption/ionization-mass spectrometry—A journey from principles and applications to a glimpse into the future

Gross

2020

Eur J Mass Spectrom (Chichester)

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The discovery of the ionizing effect of strong electric fields in the order of volts per Ångstrom in the early 1950s eventually led to the development of field ionization-mass spectrometry (FI-MS). Due to the very low ion currents, and thus, limited by the instrumentation of the 1960s, it took some time for the, by then, new technique to become adopted for analytical applications. In FI-MS, volatile or at least vaporizable samples mainly deliver molecular ions, and consequently, mass spectra showing no or at least minor numbers of fragment ion signals. The next major breakthrough was achieved by overcoming the need to evaporate the analyte prior to ionization. This was accomplished in the early 1970s by simply depositing the samples onto the field emitter and led to field desorption-mass spectrometry (FD-MS). With FD-MS, a desorption ionization method had become available that paved the road to the mass spectral analysis of larger molecules of low to high polarity and even of organic salts. In FD-MS, all of these analytes deliver spectra with no or at least few fragment ion peaks. The last milestone was the development of liquid injection field desorption/ionization (LIFDI) in the early 2000s that allows for sample deposition under the exclusion of atmospheric oxygen and water. In addition to sampling under inert conditions, LIFDI also enables more robust and quicker operation than classical FI-MS and FD-MS procedures. The development and applications of FI, FD, and LIFDI had mutual interference with the mass analyzers that were used in combination with these methods. Vice versa, the demand for using these techniques on other than magnetic sector instruments has effectuated their adaptation to different types of modern mass analyzers. The journey started with magnetic sector instruments, almost skipped quadrupole analyzers, encompassed Fourier transform ion cyclotron resonance (FT-ICR) and orthogonal acceleration time-of-flight (oaTOF) analyzers, and finally arrived at Orbitraps. Even interfaces for continuous-flow LIFDI have been realized. Even though being niche techniques to some degree, one may be confident that FI, FD, and LIFDI have a promising future ahead of them. This Account takes you on the journey from principles and applications of the title methods to a glimpse into the future.

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“…Mass spectrometry. Field desorption (16,17) mass spectra were obtained on a Kratos MS-5076 mass spectrometer at an ion accelerating voltage of 8 kV (Kratos Analytical, Ramsey, NJ). Olestra samples were loaded onto high-temperature activated, benzonitrile-coated, 10-~rn tungsten wire emitters (18) from 10-30 mg/mL chloroform solutions by the dipping technique {19).…”

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Desorption mass spectrometry of olestra

Sanders

Gardner

Lacey

et al. 1992

J Americ Oil Chem Soc

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Field desorption (FD), fast atom bombardment (FAB) and plasma desorption (PD) mass spectrometry have been used for the characterization of olestra, a mixture of octa‐, hepta‐ and hexaesters of sucrose formed by reaction of sucrose with long‐chain fatty acids (C12–C18). Most previous applications of desorption ionization mass spectrometry have involved polar compounds; however, the relatively low‐polarity olestra is also amenable to these techniques with proper sample preparation. Field desorption provides molecular weight information, but the transience of the signals limits the usefulness for observing fragmentation and measuring ester distributions. In addition, FD may not be sensitive enough to allow characterization of fractions isolated from analytical high‐performance liquid chromatography (HPLC) columns. Fast atom bombardment produces longer‐lasting signals, which permit characterization of components over a wide mass range. However, signal‐to‐noise fluctuates substantially, depending on analyte solubility in the matrix, making the characterization of partial esters collected from HPLC uncertain and difficult. Plasma desorption mass spectrometry is the easiest and most sensitive technique for olestra characterization but provides the lowest mass resolution. Because it requires no more than a few µg of material, it is effective for the characterization of HPLC fractions. Furthermore, it is the only method, of the three investigated, that allows detection of intact dimeric species having molecular masses in the 3,000 to 5,000 dalton range.

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Field desorption mass spectrometry: Applications

Cited by 42 publications

References 87 publications

Bacterial phospholipid analysis by fast atom bombardment mass spectrometry

Bacterial phospholipid analysis by fast atom bombardment mass spectrometry

From the discovery of field ionization to field desorption and liquid injection field desorption/ionization-mass spectrometry—A journey from principles and applications to a glimpse into the future

Desorption mass spectrometry of olestra

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