Field-flow fractionation of alkali-liberated nuclear polyhedrosis virus from gypsy moth Lymantria dispar linnaeus

Caldwell, Karin D.; Nguyên, Thanh Trung; Giddings, J. Calvin; Mazzone, H.M.

doi:10.1016/0166-0934(80)90022-1

Cited by 48 publications

(9 citation statements)

References 13 publications

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“…One obvious application is the analysis of viruses and virus-like particles (VLPs) used as vaccines and prospective gene delivery agents, where the unique particle fractionation characteristics of FFF provide advantage. However, despite numerous studies into the use of FFF for bacteriophage and virus analysis over a period of 30 years (Caldwell et al, 1980(Caldwell et al, , 1981Giddings et al, 1977;Litzen and Wahlund, 1989;Thielking and Kulicke, 1998;Yonker et al, 1985), the technique has so-far failed to gain widespread acceptance among commercial, academic, and regulatory groups. This lack of acceptance is because of: (i) until recently, a lack of commercial precision instrumentation; (ii) no reliable published method for virus and VLP analysis; (iii) a general lack of sufficient ''uncommitted'' samples with which to develop such a method; and (iv) concerns that the technique inherently changes the sample aggregation state because of the so-called ''adsorption effect'' (Giddings et al, 1977), wherein the sample is concentrated near a membrane during analysis, increasing the potential for membrane-sample and sample-sample induced aggregation.…”

Section: Introductionmentioning

confidence: 96%

Quantitative analysis of virus‐like particle size and distribution by field‐flow fractionation

Chuan

Fan

Lua

et al. 2007

Biotech & Bioengineering

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Asymmetric flow field-flow fractionation (AFFFF) coupled with multiple-angle light scattering (MALS) is a powerful technique showing potential for the analysis of pharmaceutically-relevant virus-like particles (VLPs). A lack of published methods, and concerns that membrane adsorption during sample fractionation may cause sample aggregation, have limited widespread acceptance. Here we report a reliable optimized method for VLP analysis using AFFFF-MALS, and benchmark it against dynamic light scattering (DLS) and transmission electron microscopy (TEM). By comparing chemically identical VLPs having very different quaternary structure, sourced from both bacteria and insect cells, we show that optimized AFFFF analysis does not cause significant aggregation, and that accurate size and distribution information can be obtained for heterogeneous samples in a way not possible with TEM and DLS. Optimized AFFFF thus provides a quantitative way to monitor batch consistency for new vaccine products, and rapidly provides unique information on the whole population of particles within a sample.

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Section: Introductionmentioning

confidence: 96%

Quantitative analysis of virus‐like particle size and distribution by field‐flow fractionation

Chuan

Fan

Lua

et al. 2007

Biotech & Bioengineering

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“…Nuclear polyhedrosis virus (NPV) was alkali-liberated from the gypsy moth Lyrnantria dispar L., and the assortment of enveloped aggregated and monomeric forms were separated by sedimentation FFF (Caldwell et al, 1980). Effective molecular weights could be assigned to the various resolved particles, and with the aid of electron microscopy they were characterized as monomers, dimers, etc.…”

Section: Virusesmentioning

confidence: 99%

Field flow fractionation in biomedical analysis

Levin

1991

Biomedical Chromatography

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Samples of biomedical interest which have been analysed by field-flow fractionation techniques are surveyed. The list begins with whole cells and microorganisms, going through viruses, nucleic acids, cell fragments and organelles, down to proteins and their aggregates. The principles of separation in the normal and steric mode of retention are illustrated, and instrumentation and techniques are described. The review concentrates mainly on the two systems of choice for biomedical applications: sedimentation and flow field-flow fractionation.

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“…On-line coupled HPLC/GC has been reported in the literature for a variety of applications (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) but not, so far as we are aware, for the determination of PAC in exhaust particulate extracts. From these reports, it seems that on-line HPLC/GC has better sensitivity, reproducibility, and ease of operation than equivalent off-line systems.…”

mentioning

confidence: 99%

Nonaqueous sedimentation field flow fractionation

Yonker¹,

Jones²,

Robertson³

1987

Anal. Chem.

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spectrometer and data system to determine tracer enrichment with high precision over a period of several hours, these samples were analyzed every hour for 5 h. Results show that the 42Ca and ^Ca isotopes were enriched by 6.7 ± 0.2% and 3.3 ± 0.2%. CONCLUSIONSThese results demonstrate that isotopic abundances of calcium in urine can be determined by FARMS with high precision (CV < 0.2%). The primary factor limiting the precision is related to the surface structure of the sample, which causes changes in the isotope effect of the particle desorption process. Analysis of other factors, such as ion statistics, electron multiplier gain, probe tip configuration, instrument stability, and the data system, suggest that isotopic abundance measurements with CV less than 0.1% can likely be made once better methods for loading the sample are developed. Despite the small uncertainty which results from the changing isotope effect occurring during particle desorp-tion, the present level of performance is adequate for quantifying stable isotopic tracers of calcium used in clinical investigations. The fact that such measurements can be made with a conventional mass spectrometer which is routinely used for a variety of other measurements, such as high mass FAB analyses, exact mass measurements, and El and Cl, is most significant. It is also notable that only very minimal sample preparation is required.

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Field-flow fractionation of alkali-liberated nuclear polyhedrosis virus from gypsy moth Lymantria dispar linnaeus

Cited by 48 publications

References 13 publications

Quantitative analysis of virus‐like particle size and distribution by field‐flow fractionation

Quantitative analysis of virus‐like particle size and distribution by field‐flow fractionation

Field flow fractionation in biomedical analysis

Nonaqueous sedimentation field flow fractionation

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