To master the transformation method and spatio-temporal variation characteristics of cultivated land quality at multiple scales, this paper constructed three spatial scales (Laixi city, Qingdao city, and Shandong province) and two temporal scales (the second survey (2007) and the third survey (2020)), and used a linear model to transform the evaluation system. Descriptive statistics, area statistics, spatial distribution, and aggregation analysis were used to explore the spatial scale variability, and the dynamic variation characteristics were analyzed. The results showed that (1) the R2 of scale transformation models are more than 0.826, which has a simple structure and strong universality; (2) with the administrative scale increases, the evaluation units’ number decreases, the spatial distribution is generally similar but progressively approximate, the high and low land levels gradually change to medium-level land, and the spatial aggregation degree is county-scale > provincial-scale > city-scale, with significant scale effect; and (3) in the past ten years, the average grade has increased from 6.26 to 6.13 in Laixi city, but still has much room for development. This study puts forward a method of spatio-temporal scale transformation and scale effect analysis for cultivated land quality, which has positive significance for improving the evaluation system, promoting land protection, and regional sustainable development.