Fighting wheat powdery mildew: from genes to fields

Wang, Bo; Meng, Ting; Xiao, Bei; Yu, Tianying; Yue, Tingyan; Jin, Yuli; Ma, Pengtao

doi:10.1007/s00122-023-04445-4

Cited by 26 publications

(5 citation statements)

References 254 publications

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“…The powdery mildew-resistant gene in SJ106 was located at the end of chromosome 6DS (4.90–8.97 Mb) using SSR and BSR-seq. In previous reports, only one, Pm45 , was identified on wheat chromosome 6D (105.5–160.4 Mb), which was far away from the gene locus we localized [ 28 ]. No Pm gene was reported in the localized interval, except for a Sr42 locus [ 33 ].…”

Section: Discussionmentioning

confidence: 93%

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Genetic Basis Identification of a NLR Gene, TaRGA5-like, That Confers Partial Powdery Mildew Resistance in Wheat SJ106

Liu,

Yang,

et al. 2024

IJMS

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Wheat powdery mildew is an important fungal disease that seriously jeopardizes wheat production, which poses a serious threat to food safety. SJ106 is a high-quality, disease-resistant spring wheat variety; this disease resistance is derived from Wheat-wheatgrass 33. In this study, the powdery mildew resistance genes in SJ106 were located at the end of chromosome 6DS, a new disease resistance locus tentatively named PmSJ106 locus. This interval was composed of a nucleotide-binding leucine-rich repeat (NLR) gene cluster containing 19 NLR genes. Five NLRs were tandem duplicated genes, and one of them (a coiled coil domain–nucleotide binding site–leucine-rich repeat (CC-NBS-LRR; CNL) type gene, TaRGA5-like) expressed 69–836-fold in SJ106 compared with the susceptible control. The genome DNA and cDNA sequences of TaRGA5-like were amplified from SJ106, which contain several nucleotide polymorphisms in LRR regions compared with susceptible individuals and Chinese Spring. Overexpression of TaRGA5-like significantly increased resistance to powdery mildew in susceptible receptor wheat Jinqiang5. However, Virus induced gene silence (VIGS) of TaRGA5-like resulted in only a small decrease of SJ106 in disease resistance, presumably compensated by other NLR duplicated genes. The results suggested that TaRGA5-like confers partial powdery mildew resistance in SJ106. As a member of the PmSJ106 locus, TaRGA5-like functioned together with other NLR duplicated genes to improve wheat resistance to powdery mildew. Wheat variety SJ106 would become a novel and potentially valuable germplasm for powdery mildew resistance.

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Section: Discussionmentioning

confidence: 93%

“…Cultivating resistant varieties is an economical, effective, and environmentally friendly measure. But due to the rapid evolution of pathogens, resistance in varieties is often overcome, resulting in disease re-emergence [ 22 , 28 ]. This requires new disease-resistant varieties with durable, broad-spectrum resistance characteristics.…”

Section: Discussionmentioning

confidence: 99%

Genetic Basis Identification of a NLR Gene, TaRGA5-like, That Confers Partial Powdery Mildew Resistance in Wheat SJ106

Liu,

Yang,

et al. 2024

IJMS

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“…Except for Pm genes, wheat relatives contain abundant yield, stress resistance, and resistant genes. Therefore, these germplasm resources can be better applied in wheat resistance breeding ( Wang et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives

Wang,

Xu,

Qie

et al. 2024

Front. Genet.

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Powdery mildew is one of the most severe diseases affecting wheat yield and quality and is caused by Blumeria graminis f. sp. tritici (Bgt). Host resistance is the preferred strategy to prevent this disease. However, the narrow genetic basis of common wheat has increased the demand for diversified germplasm resources against powdery mildew. Wheat relatives, especially the secondary gene pool of common wheat, are important gene donors in the genetic improvement of common wheat because of its abundant genetic variation and close kinship with wheat. In this study, a series of 137 wheat relatives, including 53 Triticum monococcum L. (2n = 2x = 14, AA), 6 T. urartu Thumanjan ex Gandilyan (2n = 2x = 14, AA), 9 T. timopheevii Zhuk. (2n = 4x = 28, AAGG), 66 T. aestivum subsp. spelta (2n = 6x = 42, AABBDD), and 3 Aegilops speltoides (2n = 2x = 14, SS) were systematically evaluated for their powdery mildew resistance and composition of Pm genes. Out of 137 (60.58%) accessions, 83 were resistant to Bgt isolate E09 at the seedling stage, and 116 of 137 (84.67%) wheat relatives were resistant to the mixture of Bgt isolates at the adult stage. This indicates that these accessions show a high level of resistance to powdery mildew. Some 31 markers for 23 known Pm genes were used to test these 137 accessions, and, in the results, only Pm2, Pm4, Pm6, Pm58, and Pm68 were detected. Among them, three Pm4 alleles (Pm4a, Pm4b, and Pm4f) were identified in 4 T. subsp. spelta accessions. q-RT PCR further confirmed that Pm4 alleles played a role in disease resistance in these four accessions. The phylogenetic tree showed that the kinship of Pm4 was close to Pm24 and Sr62. This study not only provides reference information and valuable germplasm resources for breeding new wheat varieties with disease resistance but also lays a foundation for enriching the genetic basis of wheat resistance to powdery mildew.

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“…The nature of all identified Pm genes is not known yet, and the distinction between race-specific and race non-specific genes is not always crystal clear. However, expression stage of resistance is one of the main attributes used to categorize resistance as adult plant resistance (race non-specific) or all-stage resistance (race-specific) (Wang et al 2023 ). Currently, the molecular structure is only known for two race non-specific resistance genes against wheat powdery mildew, namely Pm38 and Pm46 , and these genes confer pleiotropic resistance against multiple fungal diseases.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide association analysis identifies a consistent QTL for powdery mildew resistance on chromosome 3A in Nordic and Baltic spring wheat

Lin,

Islamov,

Aleliūnas

et al. 2024

Theor Appl Genet

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Key message QPm.NOBAL-3A is an important QTL providing robust adult plant powdery mildew resistance in Nordic and Baltic spring wheat, aiding sustainable crop protection and breeding. Abstract Powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici, poses a significant threat to bread wheat (Triticum aestivum L.), one of the world’s most crucial cereal crops. Enhancing cultivar resistance against this devastating disease requires a comprehensive understanding of the genetic basis of powdery mildew resistance. In this study, we performed a genome-wide association study (GWAS) using extensive field trial data from multiple environments across Estonia, Latvia, Lithuania, and Norway. The study involved a diverse panel of recent wheat cultivars and breeding lines sourced from the Baltic region and Norway. We identified a major quantitative trait locus (QTL) on chromosome 3A, designated as QPm.NOBAL-3A, which consistently conferred high resistance to powdery mildew across various environments and countries. Furthermore, the consistency of the QTL haplotype effect was validated using an independent Norwegian spring wheat panel. Subsequent greenhouse seedling inoculations with 15 representative powdery mildew isolates on a subset of the GWAS panel indicated that this QTL provides adult plant resistance and is likely of race non-specific nature. Moreover, we developed and validated KASP markers for QPm.NOBAL-3A tailored for use in breeding. These findings provide a critical foundation for marker-assisted selection in breeding programs aimed at pyramiding resistance QTL/genes to achieve durable and broad-spectrum resistance against powdery mildew.

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Fighting wheat powdery mildew: from genes to fields

Cited by 26 publications

References 254 publications

Genetic Basis Identification of a NLR Gene, TaRGA5-like, That Confers Partial Powdery Mildew Resistance in Wheat SJ106

Genetic Basis Identification of a NLR Gene, TaRGA5-like, That Confers Partial Powdery Mildew Resistance in Wheat SJ106

Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives

Genome-wide association analysis identifies a consistent QTL for powdery mildew resistance on chromosome 3A in Nordic and Baltic spring wheat

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