Final steps in juvenile hormone biosynthesis in the desert locust, Schistocerca gregaria

Marchal, Elisabeth; Zhang, Jinrui; Badisco, Liesbeth; Verlinden, Heleen; Hult, Ekaterina F.; Wielendaele, Pieter Van; Yagi, Kinji; Tobe, Stephen S.; Broeck, Jozef Vanden

doi:10.1016/j.ibmb.2010.12.007

Cited by 111 publications

(119 citation statements)

References 45 publications

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“…JHAMT has been mostly described in insects with the catalytic activity in the methylation of FA/JHA to MF/JH III (Li et al, 2013;Marchal et al, 2011;Mayoral et al, 2009;Shinoda and Itoyama, 2003;Van Ekert et al, 2014). However, in crustaceans, JHAMT has only been reported in the water flea D. pulex (Hui et al, 2010), D. magna and the shrimp N. denticulata (Sin et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The expression levels of BmJHAMT exhibited a strong correlation with the rates of JH biosynthesis, and transcriptional suppression of BmJHAMT was found to be critical for the initiation of metamorphosis (Kinjoh et al, 2007;Shinoda and Itoyama, 2003). Subsequently, several JHAMT gene were cloned and characterized from other insects in different orders, which showed the spatial and catalytic properties were similar to BmJHAMT (Minakuchi et al, 2008;Mayoral et al, 2009;Marchal et al, 2011;Niwa et al, 2008;Li et al, 2013). All these studies have displayed essential roles of JHAMT in insect metamorphosis and reproduction.…”

Section: Introductionmentioning

confidence: 96%

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The potential role of juvenile hormone acid methyltransferase in methyl farnesoate (MF) biosynthesis in the swimming crab, Portunus trituberculatus

Xie

Tian

Liu

et al. 2016

Animal Reproduction Science

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Juvenile hormone (JH) and methyl farnesoate (MF) play essential roles in the development and reproduction of insects and crustaceans respectively. Juvenile hormone acid methyltransferase (JHAMT) catalyzes the methyl esterification in insect JH biosynthesis, while the corresponding step in crustacean MF biosynthesis was long thought to be catalyzed by farnesoic acid O-methyltransferase (FAMeT). However, the new discovery of JHAMT orthologs in crustaceans indicates that JHAMT may also play essential role in the MF biosynthesis in crustaceans. Here we cloned and characterized the full-length cDNA encoding JHAMT in the swimming crab Portunus trituberculatus (PtJHAMT). Sequence and structure analysis of PtJHAMT revealed that it was composed of a 6-stranded β sheet with 9 α helices, and contained a signature Sadenosyl-L-methionine (SAM) binding motif, which is the hallmark in all SAM dependent methyltransferases (SAM-MTs). Several active sites that are critical for the interaction of SAM and JH/FA substrate were also conserved in PtJHAMT. The gene expression of PtJHAMT was highly specific to the mandibular organ, which is the sole site of MF synthesis. PtJHAMT expression significantly increased in the late-vitellogenic stage and mature stage, which suggests a possible role of PtJHAMT in modulating ovarian development. The role of PtJHAMT and PtFAMeT in MF biosynthesis was further investigated by RNA interfering (RNAi). Injection of PtJHAMT and PtFAMeT dsRNA both led to a decrease in hemolymph MF titers. Injection of PtHMGR dsRNA caused the decrease in PtJHAMT expression, but had no effect on mRNA level of PtFAMeT. Together these results suggested that JHAMT and FAMeT are both involved in the MF biosynthesis in crustaceans, while the JHAMT is highly specific to FA substrate, and FAMeT may have more catalytic functions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

The potential role of juvenile hormone acid methyltransferase in methyl farnesoate (MF) biosynthesis in the swimming crab, Portunus trituberculatus

Xie

Tian

Liu

et al. 2016

Animal Reproduction Science

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“…FJ792774), D. punctata (Helvig et al 2004; no. AY509244) and S. gregaria (Marchal et al 2011; no. HQ634703)] and found another similar gene sequence in Z. nevadensis (gene ID: Znev_06057).…”

Section: Alignment and Phylogenetic Analysis Of Cyp15a1mentioning

confidence: 99%

“…A decrease in the JH titer in A. mellifera was also achieved by JHAMT RNAi (Bomtorin et al 2014). CYP15A1, encoding JH epoxidase (Helvig et al 2004), converts methyl farnesoate into JH III, and RNAi resulted in an increase in the level of methyl farnesoate in the corpora allata and a decrease in the JH titer in S. gregaria (Marchal et al 2011). Moreover, knockdown of a JH epoxidase homolog, CYP15F1, by RNAi resulted in a decrease in presoldier differentiation from workers in the rhinotermitid termite Reticulitermes flavipes Kollar (Tarver et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Expressions of juvenile hormone biosynthetic genes during presoldier differentiation in the incipient colony of Zootermopsis nevadensis (Isoptera: Archotermopsidae)

et al. 2015

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Cyp15F1: A NOVEL CYTOCHROME P450 GENE LINKED TO JUVENILE HORMONE‐DEPENDENT CASTE DIFFERENTION IN THE TERMITE Reticulitermes flavipes

Tarver

Coy²,

Scharf

2012

Arch Insect Biochem Physiol

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Termites are eusocial insects that jointly utilize juvenile hormone (JH), pheromones, and other semiochemicals to regulate caste differentiation and achieve caste homeostasis. Prior EST sequencing from the symbiont-free gut transcriptome of Reticulitermes flavipes unexpectedly revealed a number of unique cytochrome P450 (Cyp) transcripts, including fragments of a Cyp15 family gene (Cyp15F1) with homology to other insect Cyp15s that participate in JH biosynthesis. The present study investigated the role of Cyp15F1 in termite caste polyphenism and specifically tested the hypothesis that it plays a role in JH-dependent caste differentiation. After assembling the full-length Cyp15F1 cDNA sequence, we (i) determined its mRNA tissue expression profile, (ii) investigated mRNA expression changes in response to JH and the caste-regulatory primer pheromones γ-cadinene (CAD) and γ-cadinenal (ALD), and (iii) used RNA interference (RNAi) in combination with caste differentiation bioassays to investigate gene function at the phenotype level. Cyp15F1 has ubiquitous whole-body expression (including gut tissue); is rapidly and sustainably induced from 3 h to 48 h by JH, CAD, and ALD; and functions at least in part by facilitating JH-dependent soldier caste differentiation. These findings provide the second example of a termite caste regulatory gene identified through the use of RNAi, and significantly build upon our understanding of termite caste homeostatic mechanisms. These results also reinforce the concept of environmental caste determination in termites by revealing how primer pheromones, as socioenvironmental factors, can directly influence Cyp15 expression and caste differentiation.

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Final steps in juvenile hormone biosynthesis in the desert locust, Schistocerca gregaria

Cited by 111 publications

References 45 publications

The potential role of juvenile hormone acid methyltransferase in methyl farnesoate (MF) biosynthesis in the swimming crab, Portunus trituberculatus

The potential role of juvenile hormone acid methyltransferase in methyl farnesoate (MF) biosynthesis in the swimming crab, Portunus trituberculatus

Expressions of juvenile hormone biosynthetic genes during presoldier differentiation in the incipient colony of Zootermopsis nevadensis (Isoptera: Archotermopsidae)

Cyp15F1: A NOVEL CYTOCHROME P450 GENE LINKED TO JUVENILE HORMONE‐DEPENDENT CASTE DIFFERENTION IN THE TERMITE Reticulitermes flavipes

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