Finding a suitable separation condition for TLC/FTIR analysis by using multiple-narrow-band TLC technique

Jiang, Ye; Kang, Xiaoyan; Gao, Danqing; He, Aiyong; Guo, Ruyan; Fan, Xiaokun; Zhai, Yuanzheng; Xia, Jin-Ming; Xu, Yizhuang; Noda, Isao; Wu, Jinguang

doi:10.1039/c4ra13537g

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…To address this, Jiang et al . () proposed a multi‐narrow‐band TLC approach, a high‐throughput method of selecting the appropriate mobile phase and separation conditions.…”

Section: Resultsmentioning

confidence: 99%

Extracting bio‐zinc and taurine from Pinctada martensii meat

Liu

Huang

Bai

et al. 2020

Journal of Food Science

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Recently, intensive processing of marine resources has attracted considerable attention. In order to further utilize the byproducts of aquatic shellfish (Pinctada martensii meat) with high value, this study proposes a method of extracting zinc and taurine from P. martensii meat. Zinc was first extracted from P. martensii meat with an ultrasonic crusher, and then taurine was further extracted by ultrasonic-assisted water extraction from the remaining shellfish. After optimization, the biological zinc extraction rate reached 8.63%, and the taurine extraction rate reached 0.71%. Meanwhile, the parameters for cation exchange separation and taurine purification were optimized, in which the injection volume, pH value, and elution rate were set to 8.0 mL, 4.5, and 3.0 mL/min, respectively. Ultimately, the purity the extracted taurine reached 98.16%. This study provides a novel method for the extraction of biological zinc and taurine by deep processing of shellfish meat.

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“…To address this, Jiang et al . () proposed a multi‐narrow‐band TLC approach, a high‐throughput method of selecting the appropriate mobile phase and separation conditions.…”

Section: Resultsmentioning

confidence: 99%

Extracting bio‐zinc and taurine from Pinctada martensii meat

Liu

Huang

Bai

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

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“…Although a few previous publications have created TLC multi-narrow-bands as separation lanes, − their goals were either to reduce sample spreading to yield a smaller analyte spot size or to increase sample throughput and enable parallel analysis. , The bandwidth of these methods exceeded 200 μm and so was not properly sized with respect to the dimensions (10–20 μm) of a single mammalian cell. Additionally, the lane thickness (≥200 μm) was too large to support picoliter-sized sample assays.…”

Section: Introductionmentioning

confidence: 99%

Monolithic Silica Microbands Enable Thin-Layer Chromatography Analysis of Single Cells

et al. 2022

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A picoliter thin-layer chromatography (pTLC) platform was developed for analyzing extremely miniature specimens, such as assay of the contents of a single cell of 1 picoliter volume. The pTLC chip consisted of an array of microscale bands made from highly porous monolithic silica designed to accept picoliter-scale volume samples. pTLC bands were fabricated by combining sol–gel chemistry and microfabrication technology. The width (60–80 μm) and depth (13 μm) of each band is comparable to the size of single cells and acted to reduce the lateral diffusion and confine the movement of compounds along the microbands. Ultrasmall volumes (tens of pL) of model fluorescent compounds were spotted onto the microband by a piezoelectric microdispenser and successfully separated by pTLC. The separation resolution and analyte migration were dependent on the macropore size (ranging from 0.3 to 2.3 μm), which was adjustable by changing the porogen concentration during the sol–gel process. For a 0.3 μm macropore size, attomoles of analyte were detectable by fluorescence using standard microscopy methods. The separation resolution, theoretical plate number, and separation times ranged from 1.3 to 2.1, 4 to 357, and 2 to 8 min, respectively, for the chosen model biological lipids. To demonstrate the capability of pTLC for separating analytes from single mammalian cells, cells loaded with fluorescent lipophilic dyes or sphingosine kinase reporter were spotted on microbands, and the single-cell contents separated by pTLC were detected from their fluorescence. These results demonstrate the potential of pTLC for applications in many areas where miniature specimens and high-throughput parallel analyses are needed.

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“…Previously, our group has conducted several studies for investigating stationary phases suitable for thin-layer chromatography (TLC)-FTIR analysis. [7][8][9][26][27][28][29][30][31] A suitable stationary phase for use in TLC-FTIR should have the same prerequisites as the mentioned interface in powder form for HPLC-FTIR analysis. Although several insoluble inorganic salts, such as BaF2, LaF3, AgI, ZrO2, and zinc oxide (ZnO), are transparent to IR, a number of these salts tend to adsorb water.…”

Section: Introductionmentioning

confidence: 99%

Use of CuO Particles as an Interface in LC-FTIR Analysis

et al. 2017

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In this study, the feasibility of using copper oxide (CuO) as an interface for the coupling of liquid chromatography (LC) and Fourier-transform infrared (FTIR) spectroscopy was investigated. CuO exhibits low absorption in the 4000 to 1000 cm -1 FTIR spectral region. In addition, LC-FTIR using CuO as the interface was extended to the analysis of sample mixtures containing benzamide and dioctyl sebacate; both analytes were successfully separated. After complete removal of the mobile phase, benzamide and dioctyl sebacate were successfully identified from the FTIR spectrum. Surprisingly, the interference from adsorbed water or conventionally used LC solvents in the FTIR spectrum was completely eliminated by using CuO particles as the interface in the off-line hyphenation of LC-FTIR. Based on these results, CuO demonstrates potential as an ideal interface for LC-FTIR analysis.

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Finding a suitable separation condition for TLC/FTIR analysis by using multiple-narrow-band TLC technique

Abstract: A multiple-narrow-band TLC approach is proposed to find suitable solvent for TLC analysis in a high-throughput manner.

Cited by 7 publications

References 21 publications

Extracting bio‐zinc and taurine from Pinctada martensii meat

Extracting bio‐zinc and taurine from Pinctada martensii meat

Monolithic Silica Microbands Enable Thin-Layer Chromatography Analysis of Single Cells

Use of CuO Particles as an Interface in LC-FTIR Analysis

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