Fine Mapping a Broad-Spectrum Powdery Mildew Resistance Gene in Chinese Landrace Datoumai,PmDTM, and Its Relationship withPm24

Abstract

Powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a globally important wheat disease causing severe yield losses, and deployment of resistant varieties is the preferred choice for managing this disease. Chinese wheat landrace Datoumai was resistant to 22 of 23 Bgt isolates at the seedling stage. Genetic analysis based on the inoculation of Bgt isolate E09 on the F1, F2, and F2:3 populations derived from the cross Datoumai × Huixianhong revealed that the powdery mildew resistance of Datoumai is controlled by a single dominant gene, temporarily designated as PmDTM. Bulked segregant analysis and simple sequence repeat mapping with 200 F2 plants showed that PmDTM was located in the same genetic region as Pm24 on chromosome 1DS. To fine map PmDTM, 12 critical recombinants were identified from 1,192 F2 plants and delimited PmDTM to a 0.5-cM Xhnu58800 to Xhnu59000 interval covering 180.5 Kb (38,728,125 to 38,908,656 bp) on chromosome 1DS, and only one highly confident gene, TraesCS1D02G058900, was annotated within this region. TraesCS1D02G058900 encodes a receptor-like serine/threonine-protein kinase (STK), and a 6-bp deletion in exon 5 may confer the resistance to powdery mildew. Allele frequency analysis indicated that the STK allele with 6-bp deletion was only present in three landraces (Datoumai, Chiyacao [Pm24], and Hulutou) and was absent in all of the 353 Chinese modern cultivars and 147 foreign cultivars. These results demonstrate that PmDTM is mapped to the same locus as Pm24 and can be widely used to enhance powdery mildew resistance in wheat growing regions worldwide.

Identification and Transfer of a New Powdery Mildew Resistance Gene PmCAHM from Landrace Changanhongmai into Common Wheat

“…China has abundant wheat landraces, and the desirable traits can be readily transferred from landraces to some elite common wheat cultivars. So far, five genes that confer powdery mildew resistance have been identified in Chinese wheat landraces, including Pm63 (PI628024) [31], Pm61 (Xuxusanyuehuang) [15], Pm59 (PI181356) [27], Pm47 (Hongyanglazi) [26], Pm24 (Chiyacao/Baihulu) [32,33], Pm11 (Chinese spring) [34], Pm5e (Fuzhuang 30) [35,36], and Pm2c (Laomai) [37], which were mapped on chromosomal arms of 2BL, 4AL, 7Al, 7BS, 1DS, 6BS, 7BL, and 5DS, respectively. It has been reported that chromosome 1B contains three powdery mildew resistance genes, namely Pm28, Pm32, and Pm39, and their specific locations are chromosomal arms of 1B, T1BL.1SS, and 1BL, respectively [38][39][40].…”

Disease Resistance and Genes in 146 Wheat Cultivars (Lines) from the Huang-Huai-Hai Region of China

“…Therefore, Pm21 is not suggested for use alone in wheat breeding programs although it has low frequency occurrence in some regions. It would be great if it could be pyramided with other effective Pm resistance genes such as Pm12, Pm24 [58], and Pm68 [17].…”

The Mechanism of Sesame Resistance Against Macrophomina Phaseolina Was Revealed via Comparison of Transcriptomes of Resistant and Susceptible Sesame Genotypes

“…It is found that the PmDTM gene encoding receptor-like serine/threonine-protein kinase in wheat can improve the resistance of wheat to Blumeria graminis f. sp. tritici [30]. Similarly, CsWAKL08 gene encoding a wall-associated receptor-like kinase was found to regulate resistance against Xanthomonas citri subsp.…”