Fine mapping of linear B cell epitopes on capsid protein of porcine circovirus 3

Jiang, Min; Guo, Junqing; Zhang, Gaiping; Jin, Qiao; Liu, Yankai; Rui, Jia; Wang, Aiping

doi:10.1007/s00253-020-10664-2

Cited by 23 publications

(18 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The capsid-associated protein (Cap) is the structural component of the viral capsid and is the main antigenic protein of canine circoviruses because it uses repeated subunits to compose the entire capsid structure of the virus; Cap is significantly divergent and is characterized by an N-terminal region rich in basic amino acids that may provide DNA binding activity [ 40 ]. Previously, three epitopes, NKPWH, QSLFFF, and KHSRYFT, were predicted from the capsid protein of Porcine circovirus type 3 (PCV-3) [ 41 ]. These epitopes were highly conserved B-cell epitopes and could be used to develop vaccine against PCV-3 [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previously, three epitopes, NKPWH, QSLFFF, and KHSRYFT, were predicted from the capsid protein of Porcine circovirus type 3 (PCV-3) [ 41 ]. These epitopes were highly conserved B-cell epitopes and could be used to develop vaccine against PCV-3 [ 41 ]. In another study the capsid protein has been targeted to develop vaccine against dengue-2 virus [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

Jain

Joshi

Akhtar

et al. 2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background Canine circovirus is a deadly pathogen of dogs and causes vasculitis and hemorrhagic enteritis. It causes lethal gastroenteritis in pigs, fox, and dogs. Canine circovirus genome contains two main (and opposite) transcription units which encode two open reading frames (ORFs), a replicase-associated protein (Rep) and the capsid (Cap) protein. The replicase protein and capsid protein consist of 303 amino acids and 270 amino acids respectively. Several immuno-informatics methods such as epitope screening, molecular docking, and molecular-dynamics simulations were used to craft peptide-based vaccine construct against canine circovirus. Results The vaccine construct was designed by joining the selected epitopes with adjuvants by suitable linker. The cloning and expression of the vaccine construct was also performed using in silico methods. Screening of epitopes was conducted by NetMHC server that uses ANN (Artificial neural networking) algorithm. These methods are fast and cost-effective for screening epitopes that can interact with dog leukocyte antigens (DLA) and initiate an immune response. Overall, 5 epitopes, YQHLPPFRF, YIRAKWINW, ALYRRLTLI, HLQGFVNLK, and GTMNFVARR, were selected and used to design a vaccine construct. The molecular docking and molecular dynamics simulation studies show that these epitopes can bind with DLA molecules with stability. The codon adaptation and in silico cloning studies show that the vaccine can be expressed by Escherichia coli K12 strain. Conclusion The results suggest that the vaccine construct can be useful in preventing the dogs from canine circovirus infections. However, the results need further validation by performing other in vitro and in vivo experiments.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

Jain

Joshi

Akhtar

et al. 2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Peptide-based ELISA were performed according to described previously ( 33 ). Briefly, the 96-well plates were coated with the peptides (250 ng/well) in 0.05 M carbonate–bicarbonate buffer (CBS, pH 9.6) and incubated overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Epitope Profiling Reveals the Critical Antigenic Determinants in SARS-CoV-2 RBD-Based Antigen

et al. 2021

Self Cite

View full text Add to dashboard Cite

The ongoing COVID-19 pandemic caused by SARS-CoV-2 is a huge public health crisis for the globe. The receptor-binding domain (RBD) of SARS-CoV-2 spike (S) protein plays a vital role in viral infection and serves as a major target for developing neutralizing antibodies. In this study, the antibody response to the RBD of SARS-CoV-2 S protein was analyzed by a panel of sera from animals immunized with RBD-based antigens and four linear B-cell epitope peptides (R345, R405, R450 and R465) were revealed. The immunogenicity of three immunodominant peptides (R345, R405, R465) was further accessed by peptide immunization in mice, and all of them could induced potent antibody response to SARS-CoV-2 S protein, indicating that the three determinants in the RBD were immunogenic. We further generated and characterized monoclonal antibodies (15G9, 12C10 and 10D2) binding to these epitope peptides, and finely mapped the three immunodominant epitopes using the corresponding antibodies. Neutralization assays showed that all three monoclonal antibodies had neutralization activity. Results from IFA and western blotting showed that 12C10 was a cross-reactive antibody against both of SARS-CoV-2 and SARS-CoV. Results from conservative and structural analysis showed that 350VYAWN354 was a highly conserved epitope and exposed on the surface of SARS-CoV-2 S trimer, whereas 473YQAGSTP479 located in the receptor binding motif (RBM) was variable among different SARS-CoV-2 strains. 407VRQIAP412 was a highly conserved, but cryptic epitope shared between SARS-CoV-2 and SARS-CoV. These findings provide important information for understanding the humoral antibody response to the RBD of SARS-CoV-2 S protein and may facilitate further efforts to design SARS-CoV-2 vaccines and the target of COVID-19 diagnostic.

show abstract

“…In contrast, based on the proteomic analysis of the lung tissue of 4-week-old SPF piglets infected with PCV3 derived from an infectious clone (Jiang et al, 2019), PCV3 infection upregulated interferon stimulated genes (ISG) such as OAS1, Mx1, Mx2, IFIT3, and ISG15, immunoglobulins, complement, pro-inflammatory and acute phase proteins, and proteins involved in the phagosome pathway (H. Jiang et al, 2020). While the contribution of these proteins to PCV3 pathogenesis remains to be explored, these findings are similar to in vitro studies on PCV2, where infection of PK-15 cells results in RIG-1 (Dvorak et al, 2018) and cGAS signalling, interferon-β production, and down-stream ISG expression (B. Huang et al, 2018).…”

Section: Innate Immune Responsesmentioning

confidence: 99%

“…In this study, IL-12 levels peaked at day the complement cascade were also upregulated. As the samples were examined at 28 days post infection, it appears that PCV3 infection does not down-regulate CD8 + T cell and CD4 + T cell markers (H. Jiang et al, 2020). However, a more detailed characterization of cell-mediated immunity against PCV3 is required to more fully understand viral pathogenesis (Table 2) (Figure 1).…”

Section: Cell-mediated Immune Responsesmentioning

confidence: 99%

Comparative immunopathogenesis and biology of recently discovered porcine circoviruses

Rakibuzzaman

Ramamoorthy

2021

Transbound Emerg Dis

View full text Add to dashboard Cite

Porcine circoviruses are important pathogens of production swine. Porcine circovirus type 1 (PCV1) is non-pathogenic, and discovered as a contaminant of a porcine kidney cell line, PK-15. The discovery of pathogenic variant, PCV2, occurred in the late 90s in association with post-weaning multi-systemic wasting disease syndrome (PMWS), which is characterized by wasting, respiratory signs and lymphadenopathy in weanling pigs. A new PCV type, designated as PCV3, was discovered in 2016, in pigs manifesting porcine dermatitis and nephropathy syndrome (PDNS), respiratory distress and reproductive failure. Pathological manifestations of PCV3 Infections include systemic inflammation, vasculitis and myocarditis. A fourth PCV type, PCV4, was identified in 2020 in pigs with PDNS, respiratory and enteric signs. All the pathogenic PCV types are detected in both healthy and morbid pigs. They cause chronic, systemic infections with various clinical manifestations. Dysregulation of the immune system homeostasis is a pivotal trigger for pathogenesis in porcine circoviral infections. While the study of PCV3 immunobiology is still in its infancy lessons learned from PCV2 and other circular replication-associated protein (Rep)-encoding single stranded (ss) (CRESS) DNA viruses can inform the field of exploration for PCV3. Viral interactions with the innate immune system, interference with dendritic cell function coupled with the direct loss of lymphocytes compromises both innate and adaptive immunity in PCV2 infections. Dysregulated immune responses leading to the establishment of a pro-inflammatory state, immune complex associated hypersensitivity, and the necrosis of lymphocytes and immune cells are key features of PCV3 immunopathogenesis. A critical overview of the comparative immunopathology of PCV2 and PCV3/4, and directions for future research in the field are presented in this review.

show abstract

Fine mapping of linear B cell epitopes on capsid protein of porcine circovirus 3

Cited by 23 publications

References 48 publications

An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

Epitope Profiling Reveals the Critical Antigenic Determinants in SARS-CoV-2 RBD-Based Antigen

Comparative immunopathogenesis and biology of recently discovered porcine circoviruses

Contact Info

Product

Resources

About